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Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection

We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation of chromosom...

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Detalles Bibliográficos
Autores principales:	Baba, Tomoya, Ara, Takeshi, Hasegawa, Miki, Takai, Yuki, Okumura, Yoshiko, Baba, Miki, Datsenko, Kirill A, Tomita, Masaru, Wanner, Barry L, Mori, Hirotada
Formato:	Texto
Lenguaje:	English
Publicado:	2006
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1681482/ https://www.ncbi.nlm.nih.gov/pubmed/16738554 http://dx.doi.org/10.1038/msb4100050

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