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Eliminating helper phage from phage display

Phage display technology involves the display of proteins or peptides, as coat protein fusions, on the surface of a phage or phagemid particles. Using standard technology, helper phage are essential for the replication and assembly of phagemid particles, during library production and biopanning. We...

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Detalles Bibliográficos
Autores principales: Chasteen, L., Ayriss, J., Pavlik, P., Bradbury, A. R. M.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1693883/
https://www.ncbi.nlm.nih.gov/pubmed/17088290
http://dx.doi.org/10.1093/nar/gkl772
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author Chasteen, L.
Ayriss, J.
Pavlik, P.
Bradbury, A. R. M.
author_facet Chasteen, L.
Ayriss, J.
Pavlik, P.
Bradbury, A. R. M.
author_sort Chasteen, L.
collection PubMed
description Phage display technology involves the display of proteins or peptides, as coat protein fusions, on the surface of a phage or phagemid particles. Using standard technology, helper phage are essential for the replication and assembly of phagemid particles, during library production and biopanning. We have eliminated the need to add helper phage by using 'bacterial packaging cell lines' that provide the same functions. These cell lines contain M13-based helper plasmids that express phage packaging proteins which assemble phagemid particles as efficiently as helper phage, but without helper phage contamination. This results in genetically pure phagemid particle preparations. Furthermore, by using constructs differing in the form of gene 3 that they contain, we have shown that the display, from a single library, can be modulated between monovalent (phagemid-like) and multivalent display (phage-like) without any further engineering. These packaging cells eliminate the use of helper phage from phagemid-based selection protocols; reducing the amount of technical preparation, facilitating automation, optimizing selections by matching display levels to diversity, and effectively using the packaged phagemid particles as means to transfer genetic information at an efficiency approaching 100%.
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spelling pubmed-16938832006-12-28 Eliminating helper phage from phage display Chasteen, L. Ayriss, J. Pavlik, P. Bradbury, A. R. M. Nucleic Acids Res Methods Online Phage display technology involves the display of proteins or peptides, as coat protein fusions, on the surface of a phage or phagemid particles. Using standard technology, helper phage are essential for the replication and assembly of phagemid particles, during library production and biopanning. We have eliminated the need to add helper phage by using 'bacterial packaging cell lines' that provide the same functions. These cell lines contain M13-based helper plasmids that express phage packaging proteins which assemble phagemid particles as efficiently as helper phage, but without helper phage contamination. This results in genetically pure phagemid particle preparations. Furthermore, by using constructs differing in the form of gene 3 that they contain, we have shown that the display, from a single library, can be modulated between monovalent (phagemid-like) and multivalent display (phage-like) without any further engineering. These packaging cells eliminate the use of helper phage from phagemid-based selection protocols; reducing the amount of technical preparation, facilitating automation, optimizing selections by matching display levels to diversity, and effectively using the packaged phagemid particles as means to transfer genetic information at an efficiency approaching 100%. Oxford University Press 2006-12 2006-11-06 /pmc/articles/PMC1693883/ /pubmed/17088290 http://dx.doi.org/10.1093/nar/gkl772 Text en © 2006 The Author(s)
spellingShingle Methods Online
Chasteen, L.
Ayriss, J.
Pavlik, P.
Bradbury, A. R. M.
Eliminating helper phage from phage display
title Eliminating helper phage from phage display
title_full Eliminating helper phage from phage display
title_fullStr Eliminating helper phage from phage display
title_full_unstemmed Eliminating helper phage from phage display
title_short Eliminating helper phage from phage display
title_sort eliminating helper phage from phage display
topic Methods Online
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1693883/
https://www.ncbi.nlm.nih.gov/pubmed/17088290
http://dx.doi.org/10.1093/nar/gkl772
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