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Role of metal ions in catalysis by HIV integrase analyzed using a quantitative PCR disintegration assay

Paired metal ions have been proposed to be central to the catalytic mechanisms of RNase H nucleases, bacterial transposases, Holliday junction resolvases, retroviral integrases and many other enzymes. Here we present a sensitive assay for DNA transesterification in which catalysis by human immunodef...

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Detalles Bibliográficos
Autores principales:	Diamond, Tracy L., Bushman, Frederic D.
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2006
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1693899/ https://www.ncbi.nlm.nih.gov/pubmed/17085478 http://dx.doi.org/10.1093/nar/gkl862

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