A role for Phospholipase D in Drosophila embryonic cellularization

BACKGROUND: Cellularization of the Drosophila embryo is an unusually synchronous form of cytokinesis in which polarized membrane extension proceeds in part through incorporation of new membrane via fusion of apically-translocated Golgi-derived vesicles. RESULTS: We describe here involvement of the s...

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Detalles Bibliográficos
Autores principales: LaLonde, Mary, Janssens, Hilde, Yun, Suyong, Crosby, Juan, Redina, Olga, Olive, Virginie, Altshuller, Yelena M, Choi, Seok-Yong, Du, Guangwei, Gergen, J Peter, Frohman, Michael A
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1698916/
https://www.ncbi.nlm.nih.gov/pubmed/17156430
http://dx.doi.org/10.1186/1471-213X-6-60
Descripción
Sumario:BACKGROUND: Cellularization of the Drosophila embryo is an unusually synchronous form of cytokinesis in which polarized membrane extension proceeds in part through incorporation of new membrane via fusion of apically-translocated Golgi-derived vesicles. RESULTS: We describe here involvement of the signaling enzyme Phospholipase D (Pld) in regulation of this developmental step. Functional analysis using gene targeting revealed that cellularization is hindered by the loss of Pld, resulting frequently in early embryonic developmental arrest. Mechanistically, chronic Pld deficiency causes abnormal Golgi structure and secretory vesicle trafficking. CONCLUSION: Our results suggest that Pld functions to promote trafficking of Golgi-derived fusion-competent vesicles during cellularization.

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