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Yeast homotypic vacuole fusion requires the Ccz1–Mon1 complex during the tethering/docking stage
The function of the yeast lysosome/vacuole is critically linked with the morphology of the organelle. Accordingly, highly regulated processes control vacuolar fission and fusion events. Analysis of homotypic vacuole fusion demonstrated that vacuoles from strains defective in the CCZ1 and MON1 genes...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2003
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1705953/ https://www.ncbi.nlm.nih.gov/pubmed/14662743 http://dx.doi.org/10.1083/jcb.200308071 |
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author | Wang, Chao-Wen Stromhaug, Per E. Kauffman, Emily J. Weisman, Lois S. Klionsky, Daniel J. |
author_facet | Wang, Chao-Wen Stromhaug, Per E. Kauffman, Emily J. Weisman, Lois S. Klionsky, Daniel J. |
author_sort | Wang, Chao-Wen |
collection | PubMed |
description | The function of the yeast lysosome/vacuole is critically linked with the morphology of the organelle. Accordingly, highly regulated processes control vacuolar fission and fusion events. Analysis of homotypic vacuole fusion demonstrated that vacuoles from strains defective in the CCZ1 and MON1 genes could not fuse. Morphological evidence suggested that these mutant vacuoles could not proceed to the tethering/docking stage. Ccz1 and Mon1 form a stable protein complex that binds the vacuole membrane. In the absence of the Ccz1–Mon1 complex, the integrity of vacuole SNARE pairing and the unpaired SNARE class C Vps/HOPS complex interaction were both impaired. The Ccz1–Mon1 complex colocalized with other fusion components on the vacuole as part of the cis-SNARE complex, and the association of the Ccz1–Mon1 complex with the vacuole appeared to be regulated by the class C Vps/HOPS complex proteins. Accordingly, we propose that the Ccz1–Mon1 complex is critical for the Ypt7-dependent tethering/docking stage leading to the formation of a trans-SNARE complex and subsequent vacuole fusion. |
format | Text |
id | pubmed-1705953 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2003 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-17059532008-05-01 Yeast homotypic vacuole fusion requires the Ccz1–Mon1 complex during the tethering/docking stage Wang, Chao-Wen Stromhaug, Per E. Kauffman, Emily J. Weisman, Lois S. Klionsky, Daniel J. J Cell Biol Article The function of the yeast lysosome/vacuole is critically linked with the morphology of the organelle. Accordingly, highly regulated processes control vacuolar fission and fusion events. Analysis of homotypic vacuole fusion demonstrated that vacuoles from strains defective in the CCZ1 and MON1 genes could not fuse. Morphological evidence suggested that these mutant vacuoles could not proceed to the tethering/docking stage. Ccz1 and Mon1 form a stable protein complex that binds the vacuole membrane. In the absence of the Ccz1–Mon1 complex, the integrity of vacuole SNARE pairing and the unpaired SNARE class C Vps/HOPS complex interaction were both impaired. The Ccz1–Mon1 complex colocalized with other fusion components on the vacuole as part of the cis-SNARE complex, and the association of the Ccz1–Mon1 complex with the vacuole appeared to be regulated by the class C Vps/HOPS complex proteins. Accordingly, we propose that the Ccz1–Mon1 complex is critical for the Ypt7-dependent tethering/docking stage leading to the formation of a trans-SNARE complex and subsequent vacuole fusion. The Rockefeller University Press 2003-12-08 /pmc/articles/PMC1705953/ /pubmed/14662743 http://dx.doi.org/10.1083/jcb.200308071 Text en Copyright © 2003, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Wang, Chao-Wen Stromhaug, Per E. Kauffman, Emily J. Weisman, Lois S. Klionsky, Daniel J. Yeast homotypic vacuole fusion requires the Ccz1–Mon1 complex during the tethering/docking stage |
title | Yeast homotypic vacuole fusion requires the Ccz1–Mon1 complex during the tethering/docking stage |
title_full | Yeast homotypic vacuole fusion requires the Ccz1–Mon1 complex during the tethering/docking stage |
title_fullStr | Yeast homotypic vacuole fusion requires the Ccz1–Mon1 complex during the tethering/docking stage |
title_full_unstemmed | Yeast homotypic vacuole fusion requires the Ccz1–Mon1 complex during the tethering/docking stage |
title_short | Yeast homotypic vacuole fusion requires the Ccz1–Mon1 complex during the tethering/docking stage |
title_sort | yeast homotypic vacuole fusion requires the ccz1–mon1 complex during the tethering/docking stage |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1705953/ https://www.ncbi.nlm.nih.gov/pubmed/14662743 http://dx.doi.org/10.1083/jcb.200308071 |
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