Modification of the mycobacteriophage Ms6 attP core allows the integration of multiple vectors into different tRNA(ala )T-loops in slow- and fast-growing mycobacteria

BACKGROUND: Mycobacteriophage Ms6 integrates into Mycobacterium smegmatis and M. bovis BCG chromosome at the 3' end of tRNA(ala )genes. Homologous recombination occurs between the phage attP core and the attB site located in the T-loop. Integration-proficient vectors derived from Ms6 are useful...

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Detalles Bibliográficos
Autores principales: Dos Vultos, Tiago, Méderlé, Isabelle, Abadie, Valérie, Pimentel, Madalena, Moniz-Pereira, José, Gicquel, Brigitte, Reyrat, Jean-Marc, Winter, Nathalie
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1762012/
https://www.ncbi.nlm.nih.gov/pubmed/17173678
http://dx.doi.org/10.1186/1471-2199-7-47

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