Electrochemically Generated Acid and Its Containment to 100 Micron Reaction Areas for the Production of DNA Microarrays

An addressable electrode array was used for the production of acid at sufficient concentration to allow deprotection of the dimethoxytrityl (DMT) protecting group from an overlaying substrate bound to a porous reaction layer. Containment of the generated acid to an active electrode of 100 micron dia...

Descripción completa

Detalles Bibliográficos
Autores principales: Maurer, Karl, Cooper, John, Caraballo, Marcelo, Crye, James, Suciu, Dominic, Ghindilis, Andrey, Leonetti, Joseph A., Wang, Wei, Rossi, Francis M., Stöver, Axel G., Larson, Christopher, Gao, Hetian, Dill, Kilian, McShea, Andy
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2006
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1762329/
https://www.ncbi.nlm.nih.gov/pubmed/17183662
http://dx.doi.org/10.1371/journal.pone.0000034
Descripción
Sumario:An addressable electrode array was used for the production of acid at sufficient concentration to allow deprotection of the dimethoxytrityl (DMT) protecting group from an overlaying substrate bound to a porous reaction layer. Containment of the generated acid to an active electrode of 100 micron diameter was achieved by the presence of an organic base. This procedure was then used for the production of a DNA array, in which synthesis was directed by the electrochemical removal of the DMT group during synthesis. The product array was found to have a detection sensitivity to as low as 0.5 pM DNA in a complex background sample.

Ejemplares similares