Electrochemically Generated Acid and Its Containment to 100 Micron Reaction Areas for the Production of DNA Microarrays

An addressable electrode array was used for the production of acid at sufficient concentration to allow deprotection of the dimethoxytrityl (DMT) protecting group from an overlaying substrate bound to a porous reaction layer. Containment of the generated acid to an active electrode of 100 micron dia...

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Autores principales: Maurer, Karl, Cooper, John, Caraballo, Marcelo, Crye, James, Suciu, Dominic, Ghindilis, Andrey, Leonetti, Joseph A., Wang, Wei, Rossi, Francis M., Stöver, Axel G., Larson, Christopher, Gao, Hetian, Dill, Kilian, McShea, Andy
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2006
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1762329/
https://www.ncbi.nlm.nih.gov/pubmed/17183662
http://dx.doi.org/10.1371/journal.pone.0000034
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author Maurer, Karl
Cooper, John
Caraballo, Marcelo
Crye, James
Suciu, Dominic
Ghindilis, Andrey
Leonetti, Joseph A.
Wang, Wei
Rossi, Francis M.
Stöver, Axel G.
Larson, Christopher
Gao, Hetian
Dill, Kilian
McShea, Andy
author_facet Maurer, Karl
Cooper, John
Caraballo, Marcelo
Crye, James
Suciu, Dominic
Ghindilis, Andrey
Leonetti, Joseph A.
Wang, Wei
Rossi, Francis M.
Stöver, Axel G.
Larson, Christopher
Gao, Hetian
Dill, Kilian
McShea, Andy
author_sort Maurer, Karl
collection PubMed
description An addressable electrode array was used for the production of acid at sufficient concentration to allow deprotection of the dimethoxytrityl (DMT) protecting group from an overlaying substrate bound to a porous reaction layer. Containment of the generated acid to an active electrode of 100 micron diameter was achieved by the presence of an organic base. This procedure was then used for the production of a DNA array, in which synthesis was directed by the electrochemical removal of the DMT group during synthesis. The product array was found to have a detection sensitivity to as low as 0.5 pM DNA in a complex background sample.
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spelling pubmed-17623292007-01-04 Electrochemically Generated Acid and Its Containment to 100 Micron Reaction Areas for the Production of DNA Microarrays Maurer, Karl Cooper, John Caraballo, Marcelo Crye, James Suciu, Dominic Ghindilis, Andrey Leonetti, Joseph A. Wang, Wei Rossi, Francis M. Stöver, Axel G. Larson, Christopher Gao, Hetian Dill, Kilian McShea, Andy PLoS One Research Article An addressable electrode array was used for the production of acid at sufficient concentration to allow deprotection of the dimethoxytrityl (DMT) protecting group from an overlaying substrate bound to a porous reaction layer. Containment of the generated acid to an active electrode of 100 micron diameter was achieved by the presence of an organic base. This procedure was then used for the production of a DNA array, in which synthesis was directed by the electrochemical removal of the DMT group during synthesis. The product array was found to have a detection sensitivity to as low as 0.5 pM DNA in a complex background sample. Public Library of Science 2006-12-20 /pmc/articles/PMC1762329/ /pubmed/17183662 http://dx.doi.org/10.1371/journal.pone.0000034 Text en Maurer et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Maurer, Karl
Cooper, John
Caraballo, Marcelo
Crye, James
Suciu, Dominic
Ghindilis, Andrey
Leonetti, Joseph A.
Wang, Wei
Rossi, Francis M.
Stöver, Axel G.
Larson, Christopher
Gao, Hetian
Dill, Kilian
McShea, Andy
Electrochemically Generated Acid and Its Containment to 100 Micron Reaction Areas for the Production of DNA Microarrays
title Electrochemically Generated Acid and Its Containment to 100 Micron Reaction Areas for the Production of DNA Microarrays
title_full Electrochemically Generated Acid and Its Containment to 100 Micron Reaction Areas for the Production of DNA Microarrays
title_fullStr Electrochemically Generated Acid and Its Containment to 100 Micron Reaction Areas for the Production of DNA Microarrays
title_full_unstemmed Electrochemically Generated Acid and Its Containment to 100 Micron Reaction Areas for the Production of DNA Microarrays
title_short Electrochemically Generated Acid and Its Containment to 100 Micron Reaction Areas for the Production of DNA Microarrays
title_sort electrochemically generated acid and its containment to 100 micron reaction areas for the production of dna microarrays
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1762329/
https://www.ncbi.nlm.nih.gov/pubmed/17183662
http://dx.doi.org/10.1371/journal.pone.0000034
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