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The Dark Side of EGFP: Defective Polyubiquitination

Enhanced Green Fluorescent Protein (EGFP) is the most commonly used live cell reporter despite a number of conflicting reports that it can affect cell physiology. Thus far, the precise mechanism of GFP-associated defects remained unclear. Here we demonstrate that EGFP and EGFP fusion proteins inhibi...

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Detalles Bibliográficos
Autores principales: Baens, Mathijs, Noels, Heidi, Broeckx, Vicky, Hagens, Sofie, Fevery, Sabine, Billiau, An D., Vankelecom, Hugo, Marynen, Peter
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1762387/
https://www.ncbi.nlm.nih.gov/pubmed/17183684
http://dx.doi.org/10.1371/journal.pone.0000054