High-Efficiency FLP and ΦC31 Site-Specific Recombination in Mammalian Cells

DNA site-specific recombinases (SSRs) such as Cre, FLPe, and φC31, are powerful tools for analyzing gene function in vertebrates. While the availability of multiple high-efficiency SSRs would facilitate a wide array of genomic engineering possibilities, efficient recombination in mammalian cells has...

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Detalles Bibliográficos
Autores principales: Raymond, Christopher S., Soriano, Philippe
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2007
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1764711/
https://www.ncbi.nlm.nih.gov/pubmed/17225864
http://dx.doi.org/10.1371/journal.pone.0000162
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author Raymond, Christopher S.
Soriano, Philippe
author_facet Raymond, Christopher S.
Soriano, Philippe
author_sort Raymond, Christopher S.
collection PubMed
description DNA site-specific recombinases (SSRs) such as Cre, FLPe, and φC31, are powerful tools for analyzing gene function in vertebrates. While the availability of multiple high-efficiency SSRs would facilitate a wide array of genomic engineering possibilities, efficient recombination in mammalian cells has only been observed with Cre recombinase. Here we report the de novo synthesis of mouse codon-optimized FLP (FLPo) and ΦC31 (ΦC31o) SSRs, which result in recombination efficiencies similar to Cre.
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spelling pubmed-17647112007-01-17 High-Efficiency FLP and ΦC31 Site-Specific Recombination in Mammalian Cells Raymond, Christopher S. Soriano, Philippe PLoS One Research Article DNA site-specific recombinases (SSRs) such as Cre, FLPe, and φC31, are powerful tools for analyzing gene function in vertebrates. While the availability of multiple high-efficiency SSRs would facilitate a wide array of genomic engineering possibilities, efficient recombination in mammalian cells has only been observed with Cre recombinase. Here we report the de novo synthesis of mouse codon-optimized FLP (FLPo) and ΦC31 (ΦC31o) SSRs, which result in recombination efficiencies similar to Cre. Public Library of Science 2007-01-17 /pmc/articles/PMC1764711/ /pubmed/17225864 http://dx.doi.org/10.1371/journal.pone.0000162 Text en Raymond, Soriano. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Raymond, Christopher S.
Soriano, Philippe
High-Efficiency FLP and ΦC31 Site-Specific Recombination in Mammalian Cells
title High-Efficiency FLP and ΦC31 Site-Specific Recombination in Mammalian Cells
title_full High-Efficiency FLP and ΦC31 Site-Specific Recombination in Mammalian Cells
title_fullStr High-Efficiency FLP and ΦC31 Site-Specific Recombination in Mammalian Cells
title_full_unstemmed High-Efficiency FLP and ΦC31 Site-Specific Recombination in Mammalian Cells
title_short High-Efficiency FLP and ΦC31 Site-Specific Recombination in Mammalian Cells
title_sort high-efficiency flp and φc31 site-specific recombination in mammalian cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1764711/
https://www.ncbi.nlm.nih.gov/pubmed/17225864
http://dx.doi.org/10.1371/journal.pone.0000162
work_keys_str_mv AT raymondchristophers highefficiencyflpandphc31sitespecificrecombinationinmammaliancells
AT sorianophilippe highefficiencyflpandphc31sitespecificrecombinationinmammaliancells

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