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Intracerebral administration of Interleukin-12 (IL-12) and IL-18 modifies the course of mouse scrapie

BACKGROUND: Prion diseases are characterised by a neurodegenerative pattern in which the function of immune system remains still elusive. In the present study, we evaluate if an exogenous treatment with Interleukin-12 (IL-12) and IL-18, able to activate microglia, is able to affect scrapie pathogene...

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Autores principales: Pasquali, Paolo, Nonno, Romolo, Mandara, Maria Teresa, Di Bari, Michele Angelo, Ricci, Giovanni, Petrucci, Paola, Capuccini, Silvia, Cartoni, Claudia, Macrì, Agostino, Agrimi, Umberto
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1769363/
https://www.ncbi.nlm.nih.gov/pubmed/17192191
http://dx.doi.org/10.1186/1746-6148-2-37
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author Pasquali, Paolo
Nonno, Romolo
Mandara, Maria Teresa
Di Bari, Michele Angelo
Ricci, Giovanni
Petrucci, Paola
Capuccini, Silvia
Cartoni, Claudia
Macrì, Agostino
Agrimi, Umberto
author_facet Pasquali, Paolo
Nonno, Romolo
Mandara, Maria Teresa
Di Bari, Michele Angelo
Ricci, Giovanni
Petrucci, Paola
Capuccini, Silvia
Cartoni, Claudia
Macrì, Agostino
Agrimi, Umberto
author_sort Pasquali, Paolo
collection PubMed
description BACKGROUND: Prion diseases are characterised by a neurodegenerative pattern in which the function of immune system remains still elusive. In the present study, we evaluate if an exogenous treatment with Interleukin-12 (IL-12) and IL-18, able to activate microglia, is able to affect scrapie pathogenesis. RESULTS: Cytokines injected intracranially, induced a strong inflammatory response characterised by TNF-α production and microglia activation. Two groups of mice were injected intracerebrally with high dose of ME7 strain of scrapie containing IL-12 and IL-18 or sterile saline. Cytokines-treated mice showed a more pronounced accumulation of PrP(Sc )in brain tissues at 90 days post-inoculation and a shorter mean survival times than untreated mice. CONCLUSION: We can conclude that intracerebral administration of IL-12 and IL-18 can modulate scrapie pathogenesis possibly through a microglia-mediated pattern.
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spelling pubmed-17693632007-01-13 Intracerebral administration of Interleukin-12 (IL-12) and IL-18 modifies the course of mouse scrapie Pasquali, Paolo Nonno, Romolo Mandara, Maria Teresa Di Bari, Michele Angelo Ricci, Giovanni Petrucci, Paola Capuccini, Silvia Cartoni, Claudia Macrì, Agostino Agrimi, Umberto BMC Vet Res Research Article BACKGROUND: Prion diseases are characterised by a neurodegenerative pattern in which the function of immune system remains still elusive. In the present study, we evaluate if an exogenous treatment with Interleukin-12 (IL-12) and IL-18, able to activate microglia, is able to affect scrapie pathogenesis. RESULTS: Cytokines injected intracranially, induced a strong inflammatory response characterised by TNF-α production and microglia activation. Two groups of mice were injected intracerebrally with high dose of ME7 strain of scrapie containing IL-12 and IL-18 or sterile saline. Cytokines-treated mice showed a more pronounced accumulation of PrP(Sc )in brain tissues at 90 days post-inoculation and a shorter mean survival times than untreated mice. CONCLUSION: We can conclude that intracerebral administration of IL-12 and IL-18 can modulate scrapie pathogenesis possibly through a microglia-mediated pattern. BioMed Central 2006-12-27 /pmc/articles/PMC1769363/ /pubmed/17192191 http://dx.doi.org/10.1186/1746-6148-2-37 Text en Copyright © 2006 Pasquali et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Pasquali, Paolo
Nonno, Romolo
Mandara, Maria Teresa
Di Bari, Michele Angelo
Ricci, Giovanni
Petrucci, Paola
Capuccini, Silvia
Cartoni, Claudia
Macrì, Agostino
Agrimi, Umberto
Intracerebral administration of Interleukin-12 (IL-12) and IL-18 modifies the course of mouse scrapie
title Intracerebral administration of Interleukin-12 (IL-12) and IL-18 modifies the course of mouse scrapie
title_full Intracerebral administration of Interleukin-12 (IL-12) and IL-18 modifies the course of mouse scrapie
title_fullStr Intracerebral administration of Interleukin-12 (IL-12) and IL-18 modifies the course of mouse scrapie
title_full_unstemmed Intracerebral administration of Interleukin-12 (IL-12) and IL-18 modifies the course of mouse scrapie
title_short Intracerebral administration of Interleukin-12 (IL-12) and IL-18 modifies the course of mouse scrapie
title_sort intracerebral administration of interleukin-12 (il-12) and il-18 modifies the course of mouse scrapie
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1769363/
https://www.ncbi.nlm.nih.gov/pubmed/17192191
http://dx.doi.org/10.1186/1746-6148-2-37
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