Counting colonies of clonogenic assays by using densitometric software

Clonogenic assays are a useful tool to test whether a given cancer therapy can reduce the clonogenic survival of tumour cells. A colony is defined as a cluster of at least 50 cells which can often only be determined microscopically. The process of counting colonies is very extensive work and so we d...

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Detalles Bibliográficos
Autores principales: Niyazi, Maximilian, Niyazi, Ismat, Belka, Claus
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1770926/
https://www.ncbi.nlm.nih.gov/pubmed/17212832
http://dx.doi.org/10.1186/1748-717X-2-4
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author Niyazi, Maximilian
Niyazi, Ismat
Belka, Claus
author_facet Niyazi, Maximilian
Niyazi, Ismat
Belka, Claus
author_sort Niyazi, Maximilian
collection PubMed
description Clonogenic assays are a useful tool to test whether a given cancer therapy can reduce the clonogenic survival of tumour cells. A colony is defined as a cluster of at least 50 cells which can often only be determined microscopically. The process of counting colonies is very extensive work and so we developed software that is able to count the colonies automatically from scanned flasks. This software is made freely available by us with a detailed description how to use and install the necessary features.
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spelling pubmed-17709262007-01-17 Counting colonies of clonogenic assays by using densitometric software Niyazi, Maximilian Niyazi, Ismat Belka, Claus Radiat Oncol Methodology Clonogenic assays are a useful tool to test whether a given cancer therapy can reduce the clonogenic survival of tumour cells. A colony is defined as a cluster of at least 50 cells which can often only be determined microscopically. The process of counting colonies is very extensive work and so we developed software that is able to count the colonies automatically from scanned flasks. This software is made freely available by us with a detailed description how to use and install the necessary features. BioMed Central 2007-01-09 /pmc/articles/PMC1770926/ /pubmed/17212832 http://dx.doi.org/10.1186/1748-717X-2-4 Text en Copyright © 2007 Niyazi et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology
Niyazi, Maximilian
Niyazi, Ismat
Belka, Claus
Counting colonies of clonogenic assays by using densitometric software
title Counting colonies of clonogenic assays by using densitometric software
title_full Counting colonies of clonogenic assays by using densitometric software
title_fullStr Counting colonies of clonogenic assays by using densitometric software
title_full_unstemmed Counting colonies of clonogenic assays by using densitometric software
title_short Counting colonies of clonogenic assays by using densitometric software
title_sort counting colonies of clonogenic assays by using densitometric software
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1770926/
https://www.ncbi.nlm.nih.gov/pubmed/17212832
http://dx.doi.org/10.1186/1748-717X-2-4
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