Analysis of cell-based RNAi screens

RNA interference (RNAi) screening is a powerful technology for functional characterization of biological pathways. Interpretation of RNAi screens requires computational and statistical analysis techniques. We describe a method that integrates all steps to generate a scored phenotype list from raw da...

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Detalles Bibliográficos
Autores principales: Boutros, Michael, Brás, Lígia P, Huber, Wolfgang
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Software
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1779553/
https://www.ncbi.nlm.nih.gov/pubmed/16869968
http://dx.doi.org/10.1186/gb-2006-7-7-r66
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author Boutros, Michael
Brás, Lígia P
Huber, Wolfgang
author_facet Boutros, Michael
Brás, Lígia P
Huber, Wolfgang
author_sort Boutros, Michael
collection PubMed
description RNA interference (RNAi) screening is a powerful technology for functional characterization of biological pathways. Interpretation of RNAi screens requires computational and statistical analysis techniques. We describe a method that integrates all steps to generate a scored phenotype list from raw data. It is implemented in an open-source Bioconductor/R package, cellHTS (). The method is useful for the analysis and documentation of individual RNAi screens. Moreover, it is a prerequisite for the integration of multiple experiments.
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spelling pubmed-17795532007-01-19 Analysis of cell-based RNAi screens Boutros, Michael Brás, Lígia P Huber, Wolfgang Genome Biol Software RNA interference (RNAi) screening is a powerful technology for functional characterization of biological pathways. Interpretation of RNAi screens requires computational and statistical analysis techniques. We describe a method that integrates all steps to generate a scored phenotype list from raw data. It is implemented in an open-source Bioconductor/R package, cellHTS (). The method is useful for the analysis and documentation of individual RNAi screens. Moreover, it is a prerequisite for the integration of multiple experiments. BioMed Central 2006 2006-07-25 /pmc/articles/PMC1779553/ /pubmed/16869968 http://dx.doi.org/10.1186/gb-2006-7-7-r66 Text en Copyright © 2006 Boutros et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Software
Boutros, Michael
Brás, Lígia P
Huber, Wolfgang
Analysis of cell-based RNAi screens
title Analysis of cell-based RNAi screens
title_full Analysis of cell-based RNAi screens
title_fullStr Analysis of cell-based RNAi screens
title_full_unstemmed Analysis of cell-based RNAi screens
title_short Analysis of cell-based RNAi screens
title_sort analysis of cell-based rnai screens
topic Software
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1779553/
https://www.ncbi.nlm.nih.gov/pubmed/16869968
http://dx.doi.org/10.1186/gb-2006-7-7-r66
work_keys_str_mv AT boutrosmichael analysisofcellbasedrnaiscreens
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