Detecting transcriptionally active regions using genomic tiling arrays

We have developed a method for interpreting genomic tiling array data, implemented as the program TranscriptionDetector. Probed loci expressed above background are identified by combining replicates in a way that makes minimal assumptions about the data. We performed medium-resolution Anopheles gamb...

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Detalles Bibliográficos
Autores principales: Halasz, Gabor, van Batenburg, Marinus F, Perusse, Joelle, Hua, Sujun, Lu, Xiang-Jun, White, Kevin P, Bussemaker, Harmen J
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1779562/
https://www.ncbi.nlm.nih.gov/pubmed/16859498
http://dx.doi.org/10.1186/gb-2006-7-7-r59
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author Halasz, Gabor
van Batenburg, Marinus F
Perusse, Joelle
Hua, Sujun
Lu, Xiang-Jun
White, Kevin P
Bussemaker, Harmen J
author_facet Halasz, Gabor
van Batenburg, Marinus F
Perusse, Joelle
Hua, Sujun
Lu, Xiang-Jun
White, Kevin P
Bussemaker, Harmen J
author_sort Halasz, Gabor
collection PubMed
description We have developed a method for interpreting genomic tiling array data, implemented as the program TranscriptionDetector. Probed loci expressed above background are identified by combining replicates in a way that makes minimal assumptions about the data. We performed medium-resolution Anopheles gambiae tiling array experiments and found extensive transcription of both coding and non-coding regions. Our method also showed improved detection of transcriptional units when applied to high-density tiling array data for ten human chromosomes.
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spelling pubmed-17795622007-01-19 Detecting transcriptionally active regions using genomic tiling arrays Halasz, Gabor van Batenburg, Marinus F Perusse, Joelle Hua, Sujun Lu, Xiang-Jun White, Kevin P Bussemaker, Harmen J Genome Biol Method We have developed a method for interpreting genomic tiling array data, implemented as the program TranscriptionDetector. Probed loci expressed above background are identified by combining replicates in a way that makes minimal assumptions about the data. We performed medium-resolution Anopheles gambiae tiling array experiments and found extensive transcription of both coding and non-coding regions. Our method also showed improved detection of transcriptional units when applied to high-density tiling array data for ten human chromosomes. BioMed Central 2006 2006-07-19 /pmc/articles/PMC1779562/ /pubmed/16859498 http://dx.doi.org/10.1186/gb-2006-7-7-r59 Text en Copyright © 2006 Halasz et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Method
Halasz, Gabor
van Batenburg, Marinus F
Perusse, Joelle
Hua, Sujun
Lu, Xiang-Jun
White, Kevin P
Bussemaker, Harmen J
Detecting transcriptionally active regions using genomic tiling arrays
title Detecting transcriptionally active regions using genomic tiling arrays
title_full Detecting transcriptionally active regions using genomic tiling arrays
title_fullStr Detecting transcriptionally active regions using genomic tiling arrays
title_full_unstemmed Detecting transcriptionally active regions using genomic tiling arrays
title_short Detecting transcriptionally active regions using genomic tiling arrays
title_sort detecting transcriptionally active regions using genomic tiling arrays
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1779562/
https://www.ncbi.nlm.nih.gov/pubmed/16859498
http://dx.doi.org/10.1186/gb-2006-7-7-r59
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