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Zebrafish promoter microarrays identify actively transcribed embryonic genes
We have designed a zebrafish genomic microarray to identify DNA-protein interactions in the proximal promoter regions of over 11,000 zebrafish genes. Using these microarrays, together with chromatin immunoprecipitation with an antibody directed against tri-methylated lysine 4 of Histone H3, we demon...
| Autores principales: | , , , , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2006
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1779600/ https://www.ncbi.nlm.nih.gov/pubmed/16889661 http://dx.doi.org/10.1186/gb-2006-7-8-r71 |
| _version_ | 1782131814950764544 |
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| author | Wardle, Fiona C Odom, Duncan T Bell, George W Yuan, Bingbing Danford, Timothy W Wiellette, Elizabeth L Herbolsheimer, Elizabeth Sive, Hazel L Young, Richard A Smith, James C |
| author_facet | Wardle, Fiona C Odom, Duncan T Bell, George W Yuan, Bingbing Danford, Timothy W Wiellette, Elizabeth L Herbolsheimer, Elizabeth Sive, Hazel L Young, Richard A Smith, James C |
| author_sort | Wardle, Fiona C |
| collection | PubMed |
| description | We have designed a zebrafish genomic microarray to identify DNA-protein interactions in the proximal promoter regions of over 11,000 zebrafish genes. Using these microarrays, together with chromatin immunoprecipitation with an antibody directed against tri-methylated lysine 4 of Histone H3, we demonstrate the feasibility of this method in zebrafish. This approach will allow investigators to determine the genomic binding locations of DNA interacting proteins during development and expedite the assembly of the genetic networks that regulate embryogenesis. |
| format | Text |
| id | pubmed-1779600 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2006 |
| publisher | BioMed Central |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-17796002007-01-19 Zebrafish promoter microarrays identify actively transcribed embryonic genes Wardle, Fiona C Odom, Duncan T Bell, George W Yuan, Bingbing Danford, Timothy W Wiellette, Elizabeth L Herbolsheimer, Elizabeth Sive, Hazel L Young, Richard A Smith, James C Genome Biol Method We have designed a zebrafish genomic microarray to identify DNA-protein interactions in the proximal promoter regions of over 11,000 zebrafish genes. Using these microarrays, together with chromatin immunoprecipitation with an antibody directed against tri-methylated lysine 4 of Histone H3, we demonstrate the feasibility of this method in zebrafish. This approach will allow investigators to determine the genomic binding locations of DNA interacting proteins during development and expedite the assembly of the genetic networks that regulate embryogenesis. BioMed Central 2006 2006-08-04 /pmc/articles/PMC1779600/ /pubmed/16889661 http://dx.doi.org/10.1186/gb-2006-7-8-r71 Text en Copyright © 2006 Wardle et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| spellingShingle | Method Wardle, Fiona C Odom, Duncan T Bell, George W Yuan, Bingbing Danford, Timothy W Wiellette, Elizabeth L Herbolsheimer, Elizabeth Sive, Hazel L Young, Richard A Smith, James C Zebrafish promoter microarrays identify actively transcribed embryonic genes |
| title | Zebrafish promoter microarrays identify actively transcribed embryonic genes |
| title_full | Zebrafish promoter microarrays identify actively transcribed embryonic genes |
| title_fullStr | Zebrafish promoter microarrays identify actively transcribed embryonic genes |
| title_full_unstemmed | Zebrafish promoter microarrays identify actively transcribed embryonic genes |
| title_short | Zebrafish promoter microarrays identify actively transcribed embryonic genes |
| title_sort | zebrafish promoter microarrays identify actively transcribed embryonic genes |
| topic | Method |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1779600/ https://www.ncbi.nlm.nih.gov/pubmed/16889661 http://dx.doi.org/10.1186/gb-2006-7-8-r71 |
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