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Regulation of methanol utilisation pathway genes in yeasts
Methylotrophic yeasts such as Candida boidinii, Hansenula polymorpha, Pichia methanolica and Pichia pastoris are an emerging group of eukaryotic hosts for recombinant protein production with an ever increasing number of applications during the last 30 years. Their applications are linked to the use...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2006
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1781073/ https://www.ncbi.nlm.nih.gov/pubmed/17169150 http://dx.doi.org/10.1186/1475-2859-5-39 |
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author | Hartner, Franz S Glieder, Anton |
author_facet | Hartner, Franz S Glieder, Anton |
author_sort | Hartner, Franz S |
collection | PubMed |
description | Methylotrophic yeasts such as Candida boidinii, Hansenula polymorpha, Pichia methanolica and Pichia pastoris are an emerging group of eukaryotic hosts for recombinant protein production with an ever increasing number of applications during the last 30 years. Their applications are linked to the use of strong methanol-inducible promoters derived from genes of the methanol utilisation pathway. These promoters are tightly regulated, highly repressed in presence of non-limiting concentrations of glucose in the medium and strongly induced if methanol is used as carbon source. Several factors involved in this tight control and their regulatory effects have been described so far. This review summarises available data about the regulation of promoters from methanol utilisation pathway genes. Furthermore, the role of cis and trans acting factors (e.g. transcription factors, glucose processing enzymes) in the expression of methanol utilisation pathway genes is reviewed both in the context of the native cell environment as well as in heterologous hosts. |
format | Text |
id | pubmed-1781073 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-17810732007-01-25 Regulation of methanol utilisation pathway genes in yeasts Hartner, Franz S Glieder, Anton Microb Cell Fact Review Methylotrophic yeasts such as Candida boidinii, Hansenula polymorpha, Pichia methanolica and Pichia pastoris are an emerging group of eukaryotic hosts for recombinant protein production with an ever increasing number of applications during the last 30 years. Their applications are linked to the use of strong methanol-inducible promoters derived from genes of the methanol utilisation pathway. These promoters are tightly regulated, highly repressed in presence of non-limiting concentrations of glucose in the medium and strongly induced if methanol is used as carbon source. Several factors involved in this tight control and their regulatory effects have been described so far. This review summarises available data about the regulation of promoters from methanol utilisation pathway genes. Furthermore, the role of cis and trans acting factors (e.g. transcription factors, glucose processing enzymes) in the expression of methanol utilisation pathway genes is reviewed both in the context of the native cell environment as well as in heterologous hosts. BioMed Central 2006-12-14 /pmc/articles/PMC1781073/ /pubmed/17169150 http://dx.doi.org/10.1186/1475-2859-5-39 Text en Copyright © 2006 Hartner and Glieder; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Hartner, Franz S Glieder, Anton Regulation of methanol utilisation pathway genes in yeasts |
title | Regulation of methanol utilisation pathway genes in yeasts |
title_full | Regulation of methanol utilisation pathway genes in yeasts |
title_fullStr | Regulation of methanol utilisation pathway genes in yeasts |
title_full_unstemmed | Regulation of methanol utilisation pathway genes in yeasts |
title_short | Regulation of methanol utilisation pathway genes in yeasts |
title_sort | regulation of methanol utilisation pathway genes in yeasts |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1781073/ https://www.ncbi.nlm.nih.gov/pubmed/17169150 http://dx.doi.org/10.1186/1475-2859-5-39 |
work_keys_str_mv | AT hartnerfranzs regulationofmethanolutilisationpathwaygenesinyeasts AT gliederanton regulationofmethanolutilisationpathwaygenesinyeasts |