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Simultaneous measurement of multiple Th1 and Th2 serum cytokines in psoriasis and correlation with disease severity.

BACKGROUND: Psoriatic plaques have been shown to contain increased levels of proinflammatory cytokines. Serum levels of interleukin (IL)-6, IL-7, IL-8, and interferon (IFN)-gamma have been reported elevated in psoriatic patients. AIM: To evaluate serum cytokine profiles in psoriasis patients by impr...

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Autores principales: Jacob, Sharon E, Nassiri, Mehdi, Kerdel, Francisco A, Vincek, Vladimir
Formato: Texto
Lenguaje:English
Publicado: 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1781622/
https://www.ncbi.nlm.nih.gov/pubmed/14760939
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author Jacob, Sharon E
Nassiri, Mehdi
Kerdel, Francisco A
Vincek, Vladimir
author_facet Jacob, Sharon E
Nassiri, Mehdi
Kerdel, Francisco A
Vincek, Vladimir
author_sort Jacob, Sharon E
collection PubMed
description BACKGROUND: Psoriatic plaques have been shown to contain increased levels of proinflammatory cytokines. Serum levels of interleukin (IL)-6, IL-7, IL-8, and interferon (IFN)-gamma have been reported elevated in psoriatic patients. AIM: To evaluate serum cytokine profiles in psoriasis patients by improved enzyme-linked immunosorbent assay (ELISA) technique and to correlate these levels with disease severity. METHODS: We analyzed single serum samples from 10 patients with active untreated psoriasis, two patients with active treated psoriasis, and five healthy volunteers for major T helper type 1 and T helper type 2 cytokines using the LINCOplex ELISA multi-analyte detection system that permits simultaneous detection of multiple cytokines from a single sample. The disease severity, including erythema, induration, scale, and surface area, was assessed. RESULTS: IFN-gamma was markedly elevated in all sera from psoriasis patients, 33.8 +/- 1.3 pg/ml (mean +/- standard error) versus 8 +/- 1.5 pg/ml for normal controls (p < 0.01), and positively correlated with all indices of disease severity (Spearman r > 0.6). IL-8 was also increased in psoriasis patients (24.4 +/- 1.8 pg/ml) versus normal controls (3.6 +/- 1.2 pg/ml) (p < 0.05) and positively correlated with the degree of erythema (Spearman r > 0.6). Mean IL-12 levels were decreased in sera from psoriasis patients (8.5 +/- 1.2 pg/ml) compared with normal controls (42.2 +/- 5.3 pg/ml) (p < 0.01). Also, serum IL-10 levels were below detection levels in psoriatics compared with controls (6.4 +/- 1.3 pg/ml). CONCLUSIONS: This new ELISA system allowed rapid and reliable detection of numerous cytokines in single serum samples from patients with psoriasis. We observed that IFN-gamma and IL-8 cytokines were elevated in psoriatics and correlated with parameters of disease severity while IL-10 and IL-12 were decreased.
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spelling pubmed-17816222007-01-25 Simultaneous measurement of multiple Th1 and Th2 serum cytokines in psoriasis and correlation with disease severity. Jacob, Sharon E Nassiri, Mehdi Kerdel, Francisco A Vincek, Vladimir Mediators Inflamm Research Article BACKGROUND: Psoriatic plaques have been shown to contain increased levels of proinflammatory cytokines. Serum levels of interleukin (IL)-6, IL-7, IL-8, and interferon (IFN)-gamma have been reported elevated in psoriatic patients. AIM: To evaluate serum cytokine profiles in psoriasis patients by improved enzyme-linked immunosorbent assay (ELISA) technique and to correlate these levels with disease severity. METHODS: We analyzed single serum samples from 10 patients with active untreated psoriasis, two patients with active treated psoriasis, and five healthy volunteers for major T helper type 1 and T helper type 2 cytokines using the LINCOplex ELISA multi-analyte detection system that permits simultaneous detection of multiple cytokines from a single sample. The disease severity, including erythema, induration, scale, and surface area, was assessed. RESULTS: IFN-gamma was markedly elevated in all sera from psoriasis patients, 33.8 +/- 1.3 pg/ml (mean +/- standard error) versus 8 +/- 1.5 pg/ml for normal controls (p < 0.01), and positively correlated with all indices of disease severity (Spearman r > 0.6). IL-8 was also increased in psoriasis patients (24.4 +/- 1.8 pg/ml) versus normal controls (3.6 +/- 1.2 pg/ml) (p < 0.05) and positively correlated with the degree of erythema (Spearman r > 0.6). Mean IL-12 levels were decreased in sera from psoriasis patients (8.5 +/- 1.2 pg/ml) compared with normal controls (42.2 +/- 5.3 pg/ml) (p < 0.01). Also, serum IL-10 levels were below detection levels in psoriatics compared with controls (6.4 +/- 1.3 pg/ml). CONCLUSIONS: This new ELISA system allowed rapid and reliable detection of numerous cytokines in single serum samples from patients with psoriasis. We observed that IFN-gamma and IL-8 cytokines were elevated in psoriatics and correlated with parameters of disease severity while IL-10 and IL-12 were decreased. 2003-10 /pmc/articles/PMC1781622/ /pubmed/14760939 Text en
spellingShingle Research Article
Jacob, Sharon E
Nassiri, Mehdi
Kerdel, Francisco A
Vincek, Vladimir
Simultaneous measurement of multiple Th1 and Th2 serum cytokines in psoriasis and correlation with disease severity.
title Simultaneous measurement of multiple Th1 and Th2 serum cytokines in psoriasis and correlation with disease severity.
title_full Simultaneous measurement of multiple Th1 and Th2 serum cytokines in psoriasis and correlation with disease severity.
title_fullStr Simultaneous measurement of multiple Th1 and Th2 serum cytokines in psoriasis and correlation with disease severity.
title_full_unstemmed Simultaneous measurement of multiple Th1 and Th2 serum cytokines in psoriasis and correlation with disease severity.
title_short Simultaneous measurement of multiple Th1 and Th2 serum cytokines in psoriasis and correlation with disease severity.
title_sort simultaneous measurement of multiple th1 and th2 serum cytokines in psoriasis and correlation with disease severity.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1781622/
https://www.ncbi.nlm.nih.gov/pubmed/14760939
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