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IL-1 stimulates ceramide accumulation without inducing apoptosis in intestinal epithelial cells.

BACKGROUND: In inflammatory bowel disease (IBD), cytokine levels (such as interleukin-1 (IL-1)) are elevated. We have shown previously that IL-1 activates phospholipid signaling pathways in intestinal epithelial cells (EEC), leading to increased ceramide levels. AIM: To determine whether ceramide in...

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Autores principales: Homaidan, Fadia R, El-Sabban, Marwan E, Chakroun, Iman, El-Sibai, Mirvat, Dbaibo, Ghassan S
Formato: Texto
Lenguaje:English
Publicado: 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1781644/
https://www.ncbi.nlm.nih.gov/pubmed/11926594
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author Homaidan, Fadia R
El-Sabban, Marwan E
Chakroun, Iman
El-Sibai, Mirvat
Dbaibo, Ghassan S
author_facet Homaidan, Fadia R
El-Sabban, Marwan E
Chakroun, Iman
El-Sibai, Mirvat
Dbaibo, Ghassan S
author_sort Homaidan, Fadia R
collection PubMed
description BACKGROUND: In inflammatory bowel disease (IBD), cytokine levels (such as interleukin-1 (IL-1)) are elevated. We have shown previously that IL-1 activates phospholipid signaling pathways in intestinal epithelial cells (EEC), leading to increased ceramide levels. AIM: To determine whether ceramide induces apoptosis in IEC. METHODS: Apoptosis was evaluated by annexin-V binding or Hoechst nuclear staining. Levels of bcl-2, bcl-x, bax, p53 and p21 were determined by Western blotting, and celi cycle analysis was determined by flow cytometry. RESULTS: IL-1 increased ceramide accumulation in a time-dependent and concentration-dependent manner with a peak response at 4 h, with [IL-1] = 30 ng/ml. Neither IL-1 nor ceramide induced apoptosis in EEC, but they increased bcl-2 levels and decreased bax and p21 levels without affecting bcl-x and p53 levels. They also caused a slight but significant increase in the G2/M phase. These data suggest a role for ceramide in IBD and suggest a possible mechanism for the enhanced tumorigenic activity in IBD patients.
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spelling pubmed-17816442007-01-25 IL-1 stimulates ceramide accumulation without inducing apoptosis in intestinal epithelial cells. Homaidan, Fadia R El-Sabban, Marwan E Chakroun, Iman El-Sibai, Mirvat Dbaibo, Ghassan S Mediators Inflamm Research Article BACKGROUND: In inflammatory bowel disease (IBD), cytokine levels (such as interleukin-1 (IL-1)) are elevated. We have shown previously that IL-1 activates phospholipid signaling pathways in intestinal epithelial cells (EEC), leading to increased ceramide levels. AIM: To determine whether ceramide induces apoptosis in IEC. METHODS: Apoptosis was evaluated by annexin-V binding or Hoechst nuclear staining. Levels of bcl-2, bcl-x, bax, p53 and p21 were determined by Western blotting, and celi cycle analysis was determined by flow cytometry. RESULTS: IL-1 increased ceramide accumulation in a time-dependent and concentration-dependent manner with a peak response at 4 h, with [IL-1] = 30 ng/ml. Neither IL-1 nor ceramide induced apoptosis in EEC, but they increased bcl-2 levels and decreased bax and p21 levels without affecting bcl-x and p53 levels. They also caused a slight but significant increase in the G2/M phase. These data suggest a role for ceramide in IBD and suggest a possible mechanism for the enhanced tumorigenic activity in IBD patients. 2002-02 /pmc/articles/PMC1781644/ /pubmed/11926594 Text en
spellingShingle Research Article
Homaidan, Fadia R
El-Sabban, Marwan E
Chakroun, Iman
El-Sibai, Mirvat
Dbaibo, Ghassan S
IL-1 stimulates ceramide accumulation without inducing apoptosis in intestinal epithelial cells.
title IL-1 stimulates ceramide accumulation without inducing apoptosis in intestinal epithelial cells.
title_full IL-1 stimulates ceramide accumulation without inducing apoptosis in intestinal epithelial cells.
title_fullStr IL-1 stimulates ceramide accumulation without inducing apoptosis in intestinal epithelial cells.
title_full_unstemmed IL-1 stimulates ceramide accumulation without inducing apoptosis in intestinal epithelial cells.
title_short IL-1 stimulates ceramide accumulation without inducing apoptosis in intestinal epithelial cells.
title_sort il-1 stimulates ceramide accumulation without inducing apoptosis in intestinal epithelial cells.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1781644/
https://www.ncbi.nlm.nih.gov/pubmed/11926594
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