Cargando…
Effects of the cationic protein poly-L-arginine on airway epithelial cells in vitro.
BACKGROUND: Allergic asthma is associated with an increased number of eosinophils in the airway wall. Eosinophils secrete cationic proteins, particularly major basic protein (MBP). AIM: To investigate the effect of synthetic cationic polypeptides such as poly-L-arginine, which can mimic the effect o...
Autores principales: | , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
2002
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1781659/ https://www.ncbi.nlm.nih.gov/pubmed/12137242 http://dx.doi.org/10.1080/09622935020138172 |
_version_ | 1782131933147299840 |
---|---|
author | Shahana, Shahida Kampf, Caroline Roomans, Godfried M |
author_facet | Shahana, Shahida Kampf, Caroline Roomans, Godfried M |
author_sort | Shahana, Shahida |
collection | PubMed |
description | BACKGROUND: Allergic asthma is associated with an increased number of eosinophils in the airway wall. Eosinophils secrete cationic proteins, particularly major basic protein (MBP). AIM: To investigate the effect of synthetic cationic polypeptides such as poly-L-arginine, which can mimic the effect of MBP, on airway epithelial cells. METHODS: Cultured airway epithelial cells were exposed to poly-L-arginine, and effects were determined by light and electron microscopy. RESULTS: Poly-L-arginine induced apoptosis and necrosis. Transmission electron microscopy showed mitochondrial damage and changes in the nucleus. The tight junctions were damaged, as evidenced by penetration of lanthanum. Scanning electron microscopy showed a damaged cell membrane with many pores. Microanalysis showed a significant decrease in the cellular content of magnesium, phosphorus, sodium, potassium and chlorine, and an increase in calcium. Plakoglobin immunoreactivity in the cell membrane was decreased, indicating a decrease in the number of desmosomes CONCLUSIONS: The results point to poly-L-arginine induced membrane damage, resulting in increased permeability, loss of cell-cell contacts and generalized cell damage. |
format | Text |
id | pubmed-1781659 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2002 |
record_format | MEDLINE/PubMed |
spelling | pubmed-17816592007-01-25 Effects of the cationic protein poly-L-arginine on airway epithelial cells in vitro. Shahana, Shahida Kampf, Caroline Roomans, Godfried M Mediators Inflamm Research Article BACKGROUND: Allergic asthma is associated with an increased number of eosinophils in the airway wall. Eosinophils secrete cationic proteins, particularly major basic protein (MBP). AIM: To investigate the effect of synthetic cationic polypeptides such as poly-L-arginine, which can mimic the effect of MBP, on airway epithelial cells. METHODS: Cultured airway epithelial cells were exposed to poly-L-arginine, and effects were determined by light and electron microscopy. RESULTS: Poly-L-arginine induced apoptosis and necrosis. Transmission electron microscopy showed mitochondrial damage and changes in the nucleus. The tight junctions were damaged, as evidenced by penetration of lanthanum. Scanning electron microscopy showed a damaged cell membrane with many pores. Microanalysis showed a significant decrease in the cellular content of magnesium, phosphorus, sodium, potassium and chlorine, and an increase in calcium. Plakoglobin immunoreactivity in the cell membrane was decreased, indicating a decrease in the number of desmosomes CONCLUSIONS: The results point to poly-L-arginine induced membrane damage, resulting in increased permeability, loss of cell-cell contacts and generalized cell damage. 2002-06 /pmc/articles/PMC1781659/ /pubmed/12137242 http://dx.doi.org/10.1080/09622935020138172 Text en |
spellingShingle | Research Article Shahana, Shahida Kampf, Caroline Roomans, Godfried M Effects of the cationic protein poly-L-arginine on airway epithelial cells in vitro. |
title | Effects of the cationic protein poly-L-arginine on airway epithelial cells in vitro. |
title_full | Effects of the cationic protein poly-L-arginine on airway epithelial cells in vitro. |
title_fullStr | Effects of the cationic protein poly-L-arginine on airway epithelial cells in vitro. |
title_full_unstemmed | Effects of the cationic protein poly-L-arginine on airway epithelial cells in vitro. |
title_short | Effects of the cationic protein poly-L-arginine on airway epithelial cells in vitro. |
title_sort | effects of the cationic protein poly-l-arginine on airway epithelial cells in vitro. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1781659/ https://www.ncbi.nlm.nih.gov/pubmed/12137242 http://dx.doi.org/10.1080/09622935020138172 |
work_keys_str_mv | AT shahanashahida effectsofthecationicproteinpolylarginineonairwayepithelialcellsinvitro AT kampfcaroline effectsofthecationicproteinpolylarginineonairwayepithelialcellsinvitro AT roomansgodfriedm effectsofthecationicproteinpolylarginineonairwayepithelialcellsinvitro |