Expression of a splice variant of the platelet-activating factor receptor transcript 2 in various human cancer cell lines.

Platelet-activating factor receptor (PAF-R) transcripts were analysed by reverse transcriptase-polymerase chain reaction in five human cancer cell lines derived from the breast (BT20, SKBR3 and T47D cells), the pancreas (Miapaca cells) and the bladder (5,637 cells) in order to confirm the existence...

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Detalles Bibliográficos
Autores principales: Youlyouz, Ibtissam, Magnoux, Emmanuelle, Guglielmi, Laurence, Denizot, Yves
Formato: Texto
Lenguaje:English
Publicado: 2002
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1781672/
https://www.ncbi.nlm.nih.gov/pubmed/12467527
http://dx.doi.org/10.1080/09629350210000015755
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author Youlyouz, Ibtissam
Magnoux, Emmanuelle
Guglielmi, Laurence
Denizot, Yves
author_facet Youlyouz, Ibtissam
Magnoux, Emmanuelle
Guglielmi, Laurence
Denizot, Yves
author_sort Youlyouz, Ibtissam
collection PubMed
description Platelet-activating factor receptor (PAF-R) transcripts were analysed by reverse transcriptase-polymerase chain reaction in five human cancer cell lines derived from the breast (BT20, SKBR3 and T47D cells), the pancreas (Miapaca cells) and the bladder (5,637 cells) in order to confirm the existence of a splice variant of the PAF-R transcript 2. After cloning and sequencing, we confirmed its existence in all cell lines. It consisted of the PAF-R transcript 2 lengthening with 82 nucleotides from the 3' end of exon 1 of the PAF-R gene. The role of this elongated form of the tissue-type PAF-R transcript in cell physiology remains to be elucidated.
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spelling pubmed-17816722007-01-25 Expression of a splice variant of the platelet-activating factor receptor transcript 2 in various human cancer cell lines. Youlyouz, Ibtissam Magnoux, Emmanuelle Guglielmi, Laurence Denizot, Yves Mediators Inflamm Research Article Platelet-activating factor receptor (PAF-R) transcripts were analysed by reverse transcriptase-polymerase chain reaction in five human cancer cell lines derived from the breast (BT20, SKBR3 and T47D cells), the pancreas (Miapaca cells) and the bladder (5,637 cells) in order to confirm the existence of a splice variant of the PAF-R transcript 2. After cloning and sequencing, we confirmed its existence in all cell lines. It consisted of the PAF-R transcript 2 lengthening with 82 nucleotides from the 3' end of exon 1 of the PAF-R gene. The role of this elongated form of the tissue-type PAF-R transcript in cell physiology remains to be elucidated. 2002-10 /pmc/articles/PMC1781672/ /pubmed/12467527 http://dx.doi.org/10.1080/09629350210000015755 Text en
spellingShingle Research Article
Youlyouz, Ibtissam
Magnoux, Emmanuelle
Guglielmi, Laurence
Denizot, Yves
Expression of a splice variant of the platelet-activating factor receptor transcript 2 in various human cancer cell lines.
title Expression of a splice variant of the platelet-activating factor receptor transcript 2 in various human cancer cell lines.
title_full Expression of a splice variant of the platelet-activating factor receptor transcript 2 in various human cancer cell lines.
title_fullStr Expression of a splice variant of the platelet-activating factor receptor transcript 2 in various human cancer cell lines.
title_full_unstemmed Expression of a splice variant of the platelet-activating factor receptor transcript 2 in various human cancer cell lines.
title_short Expression of a splice variant of the platelet-activating factor receptor transcript 2 in various human cancer cell lines.
title_sort expression of a splice variant of the platelet-activating factor receptor transcript 2 in various human cancer cell lines.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1781672/
https://www.ncbi.nlm.nih.gov/pubmed/12467527
http://dx.doi.org/10.1080/09629350210000015755
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AT guglielmilaurence expressionofasplicevariantoftheplateletactivatingfactorreceptortranscript2invarioushumancancercelllines
AT denizotyves expressionofasplicevariantoftheplateletactivatingfactorreceptortranscript2invarioushumancancercelllines

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