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Inflammatory cell distribution in guinea pig airways and its relationship to airway reactivity.

Although airway inflammation and airway hyperreactivity are observed after allergen inhalation both in allergic humans and animals, little is known about the mechanisms by which inflammatory cells can contribute to allergen-induced airway hyperreactivity. To understand how inflammatory cell infiltra...

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Autores principales: Westerhof, F, Timens, W, van Oosten, A, Zuidhof, A B, Nauta, N, Schuiling, M, Vos, J T, Zaagsma, J, Meurs, H, Coers, W
Formato: Texto
Lenguaje:English
Publicado: 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1781701/
https://www.ncbi.nlm.nih.gov/pubmed/11545251
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author Westerhof, F
Timens, W
van Oosten, A
Zuidhof, A B
Nauta, N
Schuiling, M
Vos, J T
Zaagsma, J
Meurs, H
Coers, W
author_facet Westerhof, F
Timens, W
van Oosten, A
Zuidhof, A B
Nauta, N
Schuiling, M
Vos, J T
Zaagsma, J
Meurs, H
Coers, W
author_sort Westerhof, F
collection PubMed
description Although airway inflammation and airway hyperreactivity are observed after allergen inhalation both in allergic humans and animals, little is known about the mechanisms by which inflammatory cells can contribute to allergen-induced airway hyperreactivity. To understand how inflammatory cell infiltration can contribute to airway hyperreactivity, the location of these cells within the airways may be crucial Using a guinea pig model of acute allergic asthma, we investigated the inflammatory cell infiltration in different airway compartments at 6 and 24 h (i.e. after the early and the late asthmatic reaction, respectively) after allergen or saline challenge in relation to changes in airway reactivity (AR) to histamine. At 6 h after allergen challenge, a threefold (p < 0.01) increase in the AR to histamine was observed. At 24 h after challenge, the AR to histamine was lower, but still significantly enhanced (1.6-fold, p < 0.05). Adventitial eosinophil and neutrophil numbers in both bronchi and bronchioli were significantly increased at 6 h post-allergen provocation as compared with saline (p < 0.01 for all), while there was a strong tendency to enhanced eosinophils in the bronchial submucosa at this time point (p = 0.08). At 24h after allergen challenge, the eosinophilic and neutrophilic cell infiltration was reduced. CD3+ T lymphocytes were increased in the adventitial compartment of the large airways (p < 0.05) and in the parenchyma (p < 0.05) at 24h post-allergen, while numbers of CD8+ cells did not differ from saline treatment at any time point post-provocation. The results indicate that, after allergen provocation, inflammatory cell numbers in the airways are mainly elevated in the adventitial compartment. The adventitial inflammation could be important for the development of allergen-induced airway hyperreactivity.
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spelling pubmed-17817012007-01-25 Inflammatory cell distribution in guinea pig airways and its relationship to airway reactivity. Westerhof, F Timens, W van Oosten, A Zuidhof, A B Nauta, N Schuiling, M Vos, J T Zaagsma, J Meurs, H Coers, W Mediators Inflamm Research Article Although airway inflammation and airway hyperreactivity are observed after allergen inhalation both in allergic humans and animals, little is known about the mechanisms by which inflammatory cells can contribute to allergen-induced airway hyperreactivity. To understand how inflammatory cell infiltration can contribute to airway hyperreactivity, the location of these cells within the airways may be crucial Using a guinea pig model of acute allergic asthma, we investigated the inflammatory cell infiltration in different airway compartments at 6 and 24 h (i.e. after the early and the late asthmatic reaction, respectively) after allergen or saline challenge in relation to changes in airway reactivity (AR) to histamine. At 6 h after allergen challenge, a threefold (p < 0.01) increase in the AR to histamine was observed. At 24 h after challenge, the AR to histamine was lower, but still significantly enhanced (1.6-fold, p < 0.05). Adventitial eosinophil and neutrophil numbers in both bronchi and bronchioli were significantly increased at 6 h post-allergen provocation as compared with saline (p < 0.01 for all), while there was a strong tendency to enhanced eosinophils in the bronchial submucosa at this time point (p = 0.08). At 24h after allergen challenge, the eosinophilic and neutrophilic cell infiltration was reduced. CD3+ T lymphocytes were increased in the adventitial compartment of the large airways (p < 0.05) and in the parenchyma (p < 0.05) at 24h post-allergen, while numbers of CD8+ cells did not differ from saline treatment at any time point post-provocation. The results indicate that, after allergen provocation, inflammatory cell numbers in the airways are mainly elevated in the adventitial compartment. The adventitial inflammation could be important for the development of allergen-induced airway hyperreactivity. 2001-06 /pmc/articles/PMC1781701/ /pubmed/11545251 Text en
spellingShingle Research Article
Westerhof, F
Timens, W
van Oosten, A
Zuidhof, A B
Nauta, N
Schuiling, M
Vos, J T
Zaagsma, J
Meurs, H
Coers, W
Inflammatory cell distribution in guinea pig airways and its relationship to airway reactivity.
title Inflammatory cell distribution in guinea pig airways and its relationship to airway reactivity.
title_full Inflammatory cell distribution in guinea pig airways and its relationship to airway reactivity.
title_fullStr Inflammatory cell distribution in guinea pig airways and its relationship to airway reactivity.
title_full_unstemmed Inflammatory cell distribution in guinea pig airways and its relationship to airway reactivity.
title_short Inflammatory cell distribution in guinea pig airways and its relationship to airway reactivity.
title_sort inflammatory cell distribution in guinea pig airways and its relationship to airway reactivity.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1781701/
https://www.ncbi.nlm.nih.gov/pubmed/11545251
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