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Permeability characteristics of human endothelial monolayers seeded on different extracellular matrix proteins.

OBJECTIVE: To investigate whether endothelial monolayer permeability changes induced by inflammatory mediators are affected by the extracellular matrix protein used for cell seeding. METHODS: Human umbilical venular endothelial cells (HUVEC) were grown to confluent monolayers on membranes coated wit...

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Detalles Bibliográficos
Autores principales: Nooteboom, A, Hendriks, T, Ottehöller, I, van der Linden, C J
Formato: Texto
Lenguaje:English
Publicado: 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1781769/
https://www.ncbi.nlm.nih.gov/pubmed/11200364
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author Nooteboom, A
Hendriks, T
Ottehöller, I
van der Linden, C J
author_facet Nooteboom, A
Hendriks, T
Ottehöller, I
van der Linden, C J
author_sort Nooteboom, A
collection PubMed
description OBJECTIVE: To investigate whether endothelial monolayer permeability changes induced by inflammatory mediators are affected by the extracellular matrix protein used for cell seeding. METHODS: Human umbilical venular endothelial cells (HUVEC) were grown to confluent monolayers on membranes coated with either collagen, fibronectin or gelatin. The permeability to albumin and dextran was then assessed, both under normal conditions and after treatment with tumor necrosis factor-alpha (TNF-alpha) and bacterial lipopolysaccharide (LPS). RESULTS: With any of the three protein coatings, tight junctions were formed all over the monolayers. The permeability of the coated membranes to albumin and dextran was reduced strongly by confluent monolayers; the relative reduction was similar for the three matrix proteins used. Pre-incubation of the monolayers with either TNF-alpha or LPS increased permeability dose dependently. However, the relative increase due to either treatment was independent of the protein used for membrane coating. CONCLUSION: The extracellular matrix protein used for initial seeding of endothelial cultures plays a minor role in determining the permeability changes induced in HUVEC monolayers by inflammatory mediators.
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spelling pubmed-17817692007-01-25 Permeability characteristics of human endothelial monolayers seeded on different extracellular matrix proteins. Nooteboom, A Hendriks, T Ottehöller, I van der Linden, C J Mediators Inflamm Research Article OBJECTIVE: To investigate whether endothelial monolayer permeability changes induced by inflammatory mediators are affected by the extracellular matrix protein used for cell seeding. METHODS: Human umbilical venular endothelial cells (HUVEC) were grown to confluent monolayers on membranes coated with either collagen, fibronectin or gelatin. The permeability to albumin and dextran was then assessed, both under normal conditions and after treatment with tumor necrosis factor-alpha (TNF-alpha) and bacterial lipopolysaccharide (LPS). RESULTS: With any of the three protein coatings, tight junctions were formed all over the monolayers. The permeability of the coated membranes to albumin and dextran was reduced strongly by confluent monolayers; the relative reduction was similar for the three matrix proteins used. Pre-incubation of the monolayers with either TNF-alpha or LPS increased permeability dose dependently. However, the relative increase due to either treatment was independent of the protein used for membrane coating. CONCLUSION: The extracellular matrix protein used for initial seeding of endothelial cultures plays a minor role in determining the permeability changes induced in HUVEC monolayers by inflammatory mediators. 2000 /pmc/articles/PMC1781769/ /pubmed/11200364 Text en
spellingShingle Research Article
Nooteboom, A
Hendriks, T
Ottehöller, I
van der Linden, C J
Permeability characteristics of human endothelial monolayers seeded on different extracellular matrix proteins.
title Permeability characteristics of human endothelial monolayers seeded on different extracellular matrix proteins.
title_full Permeability characteristics of human endothelial monolayers seeded on different extracellular matrix proteins.
title_fullStr Permeability characteristics of human endothelial monolayers seeded on different extracellular matrix proteins.
title_full_unstemmed Permeability characteristics of human endothelial monolayers seeded on different extracellular matrix proteins.
title_short Permeability characteristics of human endothelial monolayers seeded on different extracellular matrix proteins.
title_sort permeability characteristics of human endothelial monolayers seeded on different extracellular matrix proteins.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1781769/
https://www.ncbi.nlm.nih.gov/pubmed/11200364
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AT otteholleri permeabilitycharacteristicsofhumanendothelialmonolayersseededondifferentextracellularmatrixproteins
AT vanderlindencj permeabilitycharacteristicsofhumanendothelialmonolayersseededondifferentextracellularmatrixproteins