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Sera from patients with sepsis induce nitric oxide production in vascular smooth muscle cells.

BACKGROUND: Nitric oxide (NO) is an important physiological mediator of vascular tone and is involved in pathophysiology of septic shock. Although plasma nitrite is a stable end product of NO oxidation derived from endogenous NO, the plasma nitrite level is also easily affected by the intake of vari...

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Detalles Bibliográficos
Autores principales: Fujita, H, Tomiyama, J, Kudo, H, Morita, I, Murota, S
Formato: Texto
Lenguaje:English
Publicado: 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1781771/
https://www.ncbi.nlm.nih.gov/pubmed/11213912
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author Fujita, H
Tomiyama, J
Kudo, H
Morita, I
Murota, S
author_facet Fujita, H
Tomiyama, J
Kudo, H
Morita, I
Murota, S
author_sort Fujita, H
collection PubMed
description BACKGROUND: Nitric oxide (NO) is an important physiological mediator of vascular tone and is involved in pathophysiology of septic shock. Although plasma nitrite is a stable end product of NO oxidation derived from endogenous NO, the plasma nitrite level is also easily affected by the intake of various foods, bacterial products and renal functional status. AIMS: We propose an excellent alternative assay technique for measuring endogenous NO production. METHODS: We measured the nitrite level in cultured vascular smooth muscle cells (SMC) treated with serum obtained from patients with sepsis (4 patients), by means of a chemiluminescence detector. RESULTS: The nitrite concentrations in such cells were significantly higher as compared to those in the cells treated with normal serum. Moreover, the increased nitrite levels in the SMC treated with the sera obtained from patients with sepsis were completely inhibited by L-nitroarginine (1 mmol/L), a nitric oxide synthase inhibitor. CONCLUSION: These data suggest that this assay method enable us to know the ability of endogenous NO production in each patient.
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spelling pubmed-17817712007-01-25 Sera from patients with sepsis induce nitric oxide production in vascular smooth muscle cells. Fujita, H Tomiyama, J Kudo, H Morita, I Murota, S Mediators Inflamm Research Article BACKGROUND: Nitric oxide (NO) is an important physiological mediator of vascular tone and is involved in pathophysiology of septic shock. Although plasma nitrite is a stable end product of NO oxidation derived from endogenous NO, the plasma nitrite level is also easily affected by the intake of various foods, bacterial products and renal functional status. AIMS: We propose an excellent alternative assay technique for measuring endogenous NO production. METHODS: We measured the nitrite level in cultured vascular smooth muscle cells (SMC) treated with serum obtained from patients with sepsis (4 patients), by means of a chemiluminescence detector. RESULTS: The nitrite concentrations in such cells were significantly higher as compared to those in the cells treated with normal serum. Moreover, the increased nitrite levels in the SMC treated with the sera obtained from patients with sepsis were completely inhibited by L-nitroarginine (1 mmol/L), a nitric oxide synthase inhibitor. CONCLUSION: These data suggest that this assay method enable us to know the ability of endogenous NO production in each patient. 2000 /pmc/articles/PMC1781771/ /pubmed/11213912 Text en
spellingShingle Research Article
Fujita, H
Tomiyama, J
Kudo, H
Morita, I
Murota, S
Sera from patients with sepsis induce nitric oxide production in vascular smooth muscle cells.
title Sera from patients with sepsis induce nitric oxide production in vascular smooth muscle cells.
title_full Sera from patients with sepsis induce nitric oxide production in vascular smooth muscle cells.
title_fullStr Sera from patients with sepsis induce nitric oxide production in vascular smooth muscle cells.
title_full_unstemmed Sera from patients with sepsis induce nitric oxide production in vascular smooth muscle cells.
title_short Sera from patients with sepsis induce nitric oxide production in vascular smooth muscle cells.
title_sort sera from patients with sepsis induce nitric oxide production in vascular smooth muscle cells.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1781771/
https://www.ncbi.nlm.nih.gov/pubmed/11213912
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