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Interleukin-5 modulates interleukin-8 secretion in eosinophilic inflammation.

Serum and BALF (bronchoalveolar lavage fluid) IL-8 levels and serum levels were investigated in Toxocara canis infected guinea-pigs and the role of IL-5 as a modulator of cytokine secretion was studied. Serum levels increased early in infected animals, exceeding control levels 4 h after infection, p...

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Autores principales: Faccioli, L H, Medeiros, A I, Malheiro, A, Pietro, R C, Januário, A, Vargaftig, B B
Formato: Texto
Lenguaje:English
Publicado: 1998
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1781822/
https://www.ncbi.nlm.nih.gov/pubmed/9839698
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author Faccioli, L H
Medeiros, A I
Malheiro, A
Pietro, R C
Januário, A
Vargaftig, B B
author_facet Faccioli, L H
Medeiros, A I
Malheiro, A
Pietro, R C
Januário, A
Vargaftig, B B
author_sort Faccioli, L H
collection PubMed
description Serum and BALF (bronchoalveolar lavage fluid) IL-8 levels and serum levels were investigated in Toxocara canis infected guinea-pigs and the role of IL-5 as a modulator of cytokine secretion was studied. Serum levels increased early in infected animals, exceeding control levels 4 h after infection, peaked between days 6 and 18, and continued to exceed control levels after 48 days of infection. Serum and BALF IL-8 levels showed the same profile as blood eosinophilia, increasing 6 days post-infection and peaking between days 18 and 24. Treatment of infected animals with anti-IL-5 Ab suppressed eosinophilia with a parallel increase in blood IL-8 levels, whereas no change was found in levels. To support our in vivo observation we carried out experiments in vitro using guinea-pig LPS-stimulated adherent peritoneal cells which release large amounts of IL-8 into the supernatants. When rIL-5 was added to LPS-stimulated cells, 65% inhibition of IL-8 release into the supernatants was observed. Pre-incubation of cells with anti-IL-5 Ab prevented the inhibition of IL-8 release into the supernatants induced by rIL-5. Our results demonstrate for the first time that TNF-alpha and IL-8 are released concomitant with or after IL-5 in the eosinophilic inflammation induced by T. canis. Moreover, in addition to showing that IL-5 is fundamental for the induction of blood eosinophilia, the present results suggest that this cytokine may play a new biological role by acting as modulator of IL-8 secretion.
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spelling pubmed-17818222007-01-25 Interleukin-5 modulates interleukin-8 secretion in eosinophilic inflammation. Faccioli, L H Medeiros, A I Malheiro, A Pietro, R C Januário, A Vargaftig, B B Mediators Inflamm Research Article Serum and BALF (bronchoalveolar lavage fluid) IL-8 levels and serum levels were investigated in Toxocara canis infected guinea-pigs and the role of IL-5 as a modulator of cytokine secretion was studied. Serum levels increased early in infected animals, exceeding control levels 4 h after infection, peaked between days 6 and 18, and continued to exceed control levels after 48 days of infection. Serum and BALF IL-8 levels showed the same profile as blood eosinophilia, increasing 6 days post-infection and peaking between days 18 and 24. Treatment of infected animals with anti-IL-5 Ab suppressed eosinophilia with a parallel increase in blood IL-8 levels, whereas no change was found in levels. To support our in vivo observation we carried out experiments in vitro using guinea-pig LPS-stimulated adherent peritoneal cells which release large amounts of IL-8 into the supernatants. When rIL-5 was added to LPS-stimulated cells, 65% inhibition of IL-8 release into the supernatants was observed. Pre-incubation of cells with anti-IL-5 Ab prevented the inhibition of IL-8 release into the supernatants induced by rIL-5. Our results demonstrate for the first time that TNF-alpha and IL-8 are released concomitant with or after IL-5 in the eosinophilic inflammation induced by T. canis. Moreover, in addition to showing that IL-5 is fundamental for the induction of blood eosinophilia, the present results suggest that this cytokine may play a new biological role by acting as modulator of IL-8 secretion. 1998 /pmc/articles/PMC1781822/ /pubmed/9839698 Text en
spellingShingle Research Article
Faccioli, L H
Medeiros, A I
Malheiro, A
Pietro, R C
Januário, A
Vargaftig, B B
Interleukin-5 modulates interleukin-8 secretion in eosinophilic inflammation.
title Interleukin-5 modulates interleukin-8 secretion in eosinophilic inflammation.
title_full Interleukin-5 modulates interleukin-8 secretion in eosinophilic inflammation.
title_fullStr Interleukin-5 modulates interleukin-8 secretion in eosinophilic inflammation.
title_full_unstemmed Interleukin-5 modulates interleukin-8 secretion in eosinophilic inflammation.
title_short Interleukin-5 modulates interleukin-8 secretion in eosinophilic inflammation.
title_sort interleukin-5 modulates interleukin-8 secretion in eosinophilic inflammation.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1781822/
https://www.ncbi.nlm.nih.gov/pubmed/9839698
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