The effect of interleukin-10 and transforming growth factor beta-1 on HLA-DR expression in colonic epithelial cells.

The aim of this study was to assess whether interleukin-10 (IL-10) and/or transforming growth factor beta-1 (TGFbeta1) downregulate HLA-DR expression using the HT29 cell line as a model of colonic epithelial cells. HLA-DR expression was induced in HT29 cells with gamma-interferon. The effects of IL-...

Descripción completa

Detalles Bibliográficos
Autores principales: Zimmerman, M J, Radford-Smith, G R, Jewell, D P
Formato: Texto
Lenguaje:English
Publicado: 1998
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1781823/
https://www.ncbi.nlm.nih.gov/pubmed/9839692
_version_ 1782131982372700160
author Zimmerman, M J
Radford-Smith, G R
Jewell, D P
author_facet Zimmerman, M J
Radford-Smith, G R
Jewell, D P
author_sort Zimmerman, M J
collection PubMed
description The aim of this study was to assess whether interleukin-10 (IL-10) and/or transforming growth factor beta-1 (TGFbeta1) downregulate HLA-DR expression using the HT29 cell line as a model of colonic epithelial cells. HLA-DR expression was induced in HT29 cells with gamma-interferon. The effects of IL-10 alone, TGFbeta1 alone, and IL-10 and TGFbeta1 in combination were studied. HLA-DR expression was assessed using flow cytometric analysis. Gamma-interferon induced HLA-DR expression in a dose-dependent fashion. In the absence of gamma-interferon, neither IL-10 nor TGFbeta1 induced HLA-DR expression. In isolation, neither IL-10 nor TGFbeta1 downregulated HLA-DR expression. When IL-10 and TGFbeta1 were added in combination, small (6-30%) statistically significant reductions in HLA-DR expression were seen. The biological significance is unclear.
format Text
id pubmed-1781823
institution National Center for Biotechnology Information
language English
publishDate 1998
record_format MEDLINE/PubMed
spelling pubmed-17818232007-01-25 The effect of interleukin-10 and transforming growth factor beta-1 on HLA-DR expression in colonic epithelial cells. Zimmerman, M J Radford-Smith, G R Jewell, D P Mediators Inflamm Research Article The aim of this study was to assess whether interleukin-10 (IL-10) and/or transforming growth factor beta-1 (TGFbeta1) downregulate HLA-DR expression using the HT29 cell line as a model of colonic epithelial cells. HLA-DR expression was induced in HT29 cells with gamma-interferon. The effects of IL-10 alone, TGFbeta1 alone, and IL-10 and TGFbeta1 in combination were studied. HLA-DR expression was assessed using flow cytometric analysis. Gamma-interferon induced HLA-DR expression in a dose-dependent fashion. In the absence of gamma-interferon, neither IL-10 nor TGFbeta1 induced HLA-DR expression. In isolation, neither IL-10 nor TGFbeta1 downregulated HLA-DR expression. When IL-10 and TGFbeta1 were added in combination, small (6-30%) statistically significant reductions in HLA-DR expression were seen. The biological significance is unclear. 1998 /pmc/articles/PMC1781823/ /pubmed/9839692 Text en
spellingShingle Research Article
Zimmerman, M J
Radford-Smith, G R
Jewell, D P
The effect of interleukin-10 and transforming growth factor beta-1 on HLA-DR expression in colonic epithelial cells.
title The effect of interleukin-10 and transforming growth factor beta-1 on HLA-DR expression in colonic epithelial cells.
title_full The effect of interleukin-10 and transforming growth factor beta-1 on HLA-DR expression in colonic epithelial cells.
title_fullStr The effect of interleukin-10 and transforming growth factor beta-1 on HLA-DR expression in colonic epithelial cells.
title_full_unstemmed The effect of interleukin-10 and transforming growth factor beta-1 on HLA-DR expression in colonic epithelial cells.
title_short The effect of interleukin-10 and transforming growth factor beta-1 on HLA-DR expression in colonic epithelial cells.
title_sort effect of interleukin-10 and transforming growth factor beta-1 on hla-dr expression in colonic epithelial cells.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1781823/
https://www.ncbi.nlm.nih.gov/pubmed/9839692
work_keys_str_mv AT zimmermanmj theeffectofinterleukin10andtransforminggrowthfactorbeta1onhladrexpressionincolonicepithelialcells
AT radfordsmithgr theeffectofinterleukin10andtransforminggrowthfactorbeta1onhladrexpressionincolonicepithelialcells
AT jewelldp theeffectofinterleukin10andtransforminggrowthfactorbeta1onhladrexpressionincolonicepithelialcells
AT zimmermanmj effectofinterleukin10andtransforminggrowthfactorbeta1onhladrexpressionincolonicepithelialcells
AT radfordsmithgr effectofinterleukin10andtransforminggrowthfactorbeta1onhladrexpressionincolonicepithelialcells
AT jewelldp effectofinterleukin10andtransforminggrowthfactorbeta1onhladrexpressionincolonicepithelialcells

Ejemplares similares