Release of prostaglandin D2 by murine mast cells: importance of metabolite formation for antiproliferative activity.

Prostaglandin (PG) D2, PGJ2 and delta12-PGJ2 are antiproliferative eicosanoids. We investigated the production of PGD2 by murine bone marrow-derived mast cells (BMMC) taking into consideration metabolism of PGD2 to PGD2 and delta12-PGJ2. PG-metabolites were quantified by high performance liquid chro...

Descripción completa

Detalles Bibliográficos
Autores principales: Haberl, C, Hültner, L, Flügel, A, Falk, M, Geuenich, S, Wilmanns, W, Denzlinger, C
Formato: Texto
Lenguaje:English
Publicado: 1998
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1781833/
https://www.ncbi.nlm.nih.gov/pubmed/9836493
_version_ 1782131985264672768
author Haberl, C
Hültner, L
Flügel, A
Falk, M
Geuenich, S
Wilmanns, W
Denzlinger, C
author_facet Haberl, C
Hültner, L
Flügel, A
Falk, M
Geuenich, S
Wilmanns, W
Denzlinger, C
author_sort Haberl, C
collection PubMed
description Prostaglandin (PG) D2, PGJ2 and delta12-PGJ2 are antiproliferative eicosanoids. We investigated the production of PGD2 by murine bone marrow-derived mast cells (BMMC) taking into consideration metabolism of PGD2 to PGD2 and delta12-PGJ2. PG-metabolites were quantified by high performance liquid chromatography (HPLC) combined with radioimmunoassay (RIA). Stimulated with calcium ionophore A23187 BMMC released eight-fold more PGJ2 and delta12-PGJ2 than PGD2. Conversion of endogenously produced PGD2 to PGJ2 and delta12-PGJ2 proceeded rapidly in contrast to metabolism of exogenously added PGD2. The antiproliferative potency of these prostaglandins is demonstrated in vitro. We conclude that determination of PGD2 production by mast cells must take into consideration rapid conversion to active derivatives, which may play a significant role in growth regulation.
format Text
id pubmed-1781833
institution National Center for Biotechnology Information
language English
publishDate 1998
record_format MEDLINE/PubMed
spelling pubmed-17818332007-01-25 Release of prostaglandin D2 by murine mast cells: importance of metabolite formation for antiproliferative activity. Haberl, C Hültner, L Flügel, A Falk, M Geuenich, S Wilmanns, W Denzlinger, C Mediators Inflamm Research Article Prostaglandin (PG) D2, PGJ2 and delta12-PGJ2 are antiproliferative eicosanoids. We investigated the production of PGD2 by murine bone marrow-derived mast cells (BMMC) taking into consideration metabolism of PGD2 to PGD2 and delta12-PGJ2. PG-metabolites were quantified by high performance liquid chromatography (HPLC) combined with radioimmunoassay (RIA). Stimulated with calcium ionophore A23187 BMMC released eight-fold more PGJ2 and delta12-PGJ2 than PGD2. Conversion of endogenously produced PGD2 to PGJ2 and delta12-PGJ2 proceeded rapidly in contrast to metabolism of exogenously added PGD2. The antiproliferative potency of these prostaglandins is demonstrated in vitro. We conclude that determination of PGD2 production by mast cells must take into consideration rapid conversion to active derivatives, which may play a significant role in growth regulation. 1998 /pmc/articles/PMC1781833/ /pubmed/9836493 Text en
spellingShingle Research Article
Haberl, C
Hültner, L
Flügel, A
Falk, M
Geuenich, S
Wilmanns, W
Denzlinger, C
Release of prostaglandin D2 by murine mast cells: importance of metabolite formation for antiproliferative activity.
title Release of prostaglandin D2 by murine mast cells: importance of metabolite formation for antiproliferative activity.
title_full Release of prostaglandin D2 by murine mast cells: importance of metabolite formation for antiproliferative activity.
title_fullStr Release of prostaglandin D2 by murine mast cells: importance of metabolite formation for antiproliferative activity.
title_full_unstemmed Release of prostaglandin D2 by murine mast cells: importance of metabolite formation for antiproliferative activity.
title_short Release of prostaglandin D2 by murine mast cells: importance of metabolite formation for antiproliferative activity.
title_sort release of prostaglandin d2 by murine mast cells: importance of metabolite formation for antiproliferative activity.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1781833/
https://www.ncbi.nlm.nih.gov/pubmed/9836493
work_keys_str_mv AT haberlc releaseofprostaglandind2bymurinemastcellsimportanceofmetaboliteformationforantiproliferativeactivity
AT hultnerl releaseofprostaglandind2bymurinemastcellsimportanceofmetaboliteformationforantiproliferativeactivity
AT flugela releaseofprostaglandind2bymurinemastcellsimportanceofmetaboliteformationforantiproliferativeactivity
AT falkm releaseofprostaglandind2bymurinemastcellsimportanceofmetaboliteformationforantiproliferativeactivity
AT geuenichs releaseofprostaglandind2bymurinemastcellsimportanceofmetaboliteformationforantiproliferativeactivity
AT wilmannsw releaseofprostaglandind2bymurinemastcellsimportanceofmetaboliteformationforantiproliferativeactivity
AT denzlingerc releaseofprostaglandind2bymurinemastcellsimportanceofmetaboliteformationforantiproliferativeactivity

Ejemplares similares