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Reversible Unfolding of the Severe Acute Respiratory Syndrome Coronavirus Main Protease in Guanidinium Chloride

Chemical denaturant sensitivity of the dimeric main protease from severe acute respiratory syndrome (SARS) coronavirus to guanidinium chloride was examined in terms of fluorescence spectroscopy, circular dichroism, analytical ultracentrifuge, and enzyme activity change. The dimeric enzyme dissociate...

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Detalles Bibliográficos
Autores principales:	Chang, Hui-Ping, Chou, Chi-Yuan, Chang, Gu-Gang
Formato:	Texto
Lenguaje:	English
Publicado:	Biophysical Society 2007
Materias:	Proteins
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1783898/ https://www.ncbi.nlm.nih.gov/pubmed/17142288 http://dx.doi.org/10.1529/biophysj.106.091736

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