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Evaluation of the Strep B OIA test compared to standard culture methods for detection of group B streptococci.

OBJECTIVE: This study evaluated the accuracy of the commercial product Strep B OIA (optical immunoassay) compared to the standard agar and broth culture methods for detecting vaginal colonization with group B streptococcus (GBS). METHODS: Preoperative vaginal cultures were obtained from 141 nonpregn...

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Detalles Bibliográficos
Autores principales: Song, J Y, Lin, L L, Shott, S, Kimber, N, Tangora, J, Cohen, A, Wells, A, Maezes, M, Aroutcheva, A, Faro, S
Formato: Texto
Lenguaje:English
Publicado: 1999
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1784740/
https://www.ncbi.nlm.nih.gov/pubmed/10449270
http://dx.doi.org/10.1002/(SICI)1098-0997(1999)7:4<202::AID-IDOG8>3.0.CO;2-D
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author Song, J Y
Lin, L L
Shott, S
Kimber, N
Tangora, J
Cohen, A
Wells, A
Maezes, M
Aroutcheva, A
Faro, S
author_facet Song, J Y
Lin, L L
Shott, S
Kimber, N
Tangora, J
Cohen, A
Wells, A
Maezes, M
Aroutcheva, A
Faro, S
author_sort Song, J Y
collection PubMed
description OBJECTIVE: This study evaluated the accuracy of the commercial product Strep B OIA (optical immunoassay) compared to the standard agar and broth culture methods for detecting vaginal colonization with group B streptococcus (GBS). METHODS: Preoperative vaginal cultures were obtained from 141 nonpregnant gynecological patients undergoing major gynecologic surgery. Major gynecologic surgery was defined as benign gynecologic, gyne-oncology, and urogynecologic procedures. The results of the Strep B OIA test were compared to the results obtained from SXT agar (selective for GBS), colistin-nalidixic acid ((CNA) agar, and Todd-Hewitt broth cultures. RESULTS: The prevalence of vaginal GBS colonization in this population was 20.6%. The sample sensitivity and specificity of the OIA method were 58.6% and 85.7%, respectively. These values are lower than the sensitivity and specificity of 85.4% and 91.5%, respectively, given in the OIA package insert. Although the sample negative predictive value was fairly high (88.9%), the positive predictive value was low (51.5%). CONCLUSION: Although a previous study stated that the product Strep B OIA reduces the time required to obtain results (30 minutes versus days) and can, therefore, function as a useful diagnostic tool in the management of early-onset GBS disease, the present study's finding of low sensitivity and low positive predictive value indicates that this test may have very limited clinical value.
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spelling pubmed-17847402007-02-05 Evaluation of the Strep B OIA test compared to standard culture methods for detection of group B streptococci. Song, J Y Lin, L L Shott, S Kimber, N Tangora, J Cohen, A Wells, A Maezes, M Aroutcheva, A Faro, S Infect Dis Obstet Gynecol Research Article OBJECTIVE: This study evaluated the accuracy of the commercial product Strep B OIA (optical immunoassay) compared to the standard agar and broth culture methods for detecting vaginal colonization with group B streptococcus (GBS). METHODS: Preoperative vaginal cultures were obtained from 141 nonpregnant gynecological patients undergoing major gynecologic surgery. Major gynecologic surgery was defined as benign gynecologic, gyne-oncology, and urogynecologic procedures. The results of the Strep B OIA test were compared to the results obtained from SXT agar (selective for GBS), colistin-nalidixic acid ((CNA) agar, and Todd-Hewitt broth cultures. RESULTS: The prevalence of vaginal GBS colonization in this population was 20.6%. The sample sensitivity and specificity of the OIA method were 58.6% and 85.7%, respectively. These values are lower than the sensitivity and specificity of 85.4% and 91.5%, respectively, given in the OIA package insert. Although the sample negative predictive value was fairly high (88.9%), the positive predictive value was low (51.5%). CONCLUSION: Although a previous study stated that the product Strep B OIA reduces the time required to obtain results (30 minutes versus days) and can, therefore, function as a useful diagnostic tool in the management of early-onset GBS disease, the present study's finding of low sensitivity and low positive predictive value indicates that this test may have very limited clinical value. 1999 /pmc/articles/PMC1784740/ /pubmed/10449270 http://dx.doi.org/10.1002/(SICI)1098-0997(1999)7:4<202::AID-IDOG8>3.0.CO;2-D Text en
spellingShingle Research Article
Song, J Y
Lin, L L
Shott, S
Kimber, N
Tangora, J
Cohen, A
Wells, A
Maezes, M
Aroutcheva, A
Faro, S
Evaluation of the Strep B OIA test compared to standard culture methods for detection of group B streptococci.
title Evaluation of the Strep B OIA test compared to standard culture methods for detection of group B streptococci.
title_full Evaluation of the Strep B OIA test compared to standard culture methods for detection of group B streptococci.
title_fullStr Evaluation of the Strep B OIA test compared to standard culture methods for detection of group B streptococci.
title_full_unstemmed Evaluation of the Strep B OIA test compared to standard culture methods for detection of group B streptococci.
title_short Evaluation of the Strep B OIA test compared to standard culture methods for detection of group B streptococci.
title_sort evaluation of the strep b oia test compared to standard culture methods for detection of group b streptococci.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1784740/
https://www.ncbi.nlm.nih.gov/pubmed/10449270
http://dx.doi.org/10.1002/(SICI)1098-0997(1999)7:4<202::AID-IDOG8>3.0.CO;2-D
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