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Identification of rat lung-specific microRNAs by micoRNA microarray: valuable discoveries for the facilitation of lung research
BACKGROUND: An important mechanism for gene regulation utilizes small non-coding RNAs called microRNAs (miRNAs). These small RNAs play important roles in tissue development, cell differentiation and proliferation, lipid and fat metabolism, stem cells, exocytosis, diseases and cancers. To date, relat...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1790902/ https://www.ncbi.nlm.nih.gov/pubmed/17250765 http://dx.doi.org/10.1186/1471-2164-8-29 |
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author | Wang, Yang Weng, Tingting Gou, Deming Chen, Zhongming Chintagari, Narendranath Reddy Liu, Lin |
author_facet | Wang, Yang Weng, Tingting Gou, Deming Chen, Zhongming Chintagari, Narendranath Reddy Liu, Lin |
author_sort | Wang, Yang |
collection | PubMed |
description | BACKGROUND: An important mechanism for gene regulation utilizes small non-coding RNAs called microRNAs (miRNAs). These small RNAs play important roles in tissue development, cell differentiation and proliferation, lipid and fat metabolism, stem cells, exocytosis, diseases and cancers. To date, relatively little is known about functions of miRNAs in the lung except lung cancer. RESULTS: In this study, we utilized a rat miRNA microarray containing 216 miRNA probes, printed in-house, to detect the expression of miRNAs in the rat lung compared to the rat heart, brain, liver, kidney and spleen. Statistical analysis using Significant Analysis of Microarray (SAM) and Tukey Honestly Significant Difference (HSD) revealed 2 miRNAs (miR-195 and miR-200c) expressed specifically in the lung and 9 miRNAs co-expressed in the lung and another organ. 12 selected miRNAs were verified by Northern blot analysis. CONCLUSION: The identified lung-specific miRNAs from this work will facilitate functional studies of miRNAs during normal physiological and pathophysiological processes of the lung. |
format | Text |
id | pubmed-1790902 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-17909022007-02-03 Identification of rat lung-specific microRNAs by micoRNA microarray: valuable discoveries for the facilitation of lung research Wang, Yang Weng, Tingting Gou, Deming Chen, Zhongming Chintagari, Narendranath Reddy Liu, Lin BMC Genomics Research Article BACKGROUND: An important mechanism for gene regulation utilizes small non-coding RNAs called microRNAs (miRNAs). These small RNAs play important roles in tissue development, cell differentiation and proliferation, lipid and fat metabolism, stem cells, exocytosis, diseases and cancers. To date, relatively little is known about functions of miRNAs in the lung except lung cancer. RESULTS: In this study, we utilized a rat miRNA microarray containing 216 miRNA probes, printed in-house, to detect the expression of miRNAs in the rat lung compared to the rat heart, brain, liver, kidney and spleen. Statistical analysis using Significant Analysis of Microarray (SAM) and Tukey Honestly Significant Difference (HSD) revealed 2 miRNAs (miR-195 and miR-200c) expressed specifically in the lung and 9 miRNAs co-expressed in the lung and another organ. 12 selected miRNAs were verified by Northern blot analysis. CONCLUSION: The identified lung-specific miRNAs from this work will facilitate functional studies of miRNAs during normal physiological and pathophysiological processes of the lung. BioMed Central 2007-01-24 /pmc/articles/PMC1790902/ /pubmed/17250765 http://dx.doi.org/10.1186/1471-2164-8-29 Text en Copyright © 2007 Wang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wang, Yang Weng, Tingting Gou, Deming Chen, Zhongming Chintagari, Narendranath Reddy Liu, Lin Identification of rat lung-specific microRNAs by micoRNA microarray: valuable discoveries for the facilitation of lung research |
title | Identification of rat lung-specific microRNAs by micoRNA microarray: valuable discoveries for the facilitation of lung research |
title_full | Identification of rat lung-specific microRNAs by micoRNA microarray: valuable discoveries for the facilitation of lung research |
title_fullStr | Identification of rat lung-specific microRNAs by micoRNA microarray: valuable discoveries for the facilitation of lung research |
title_full_unstemmed | Identification of rat lung-specific microRNAs by micoRNA microarray: valuable discoveries for the facilitation of lung research |
title_short | Identification of rat lung-specific microRNAs by micoRNA microarray: valuable discoveries for the facilitation of lung research |
title_sort | identification of rat lung-specific micrornas by micorna microarray: valuable discoveries for the facilitation of lung research |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1790902/ https://www.ncbi.nlm.nih.gov/pubmed/17250765 http://dx.doi.org/10.1186/1471-2164-8-29 |
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