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Immune response after experimental allergic encephalomyelitis in rats subjected to calorie restriction

Male Lewis rats (6 weeks-old) were submitted to a calorie restriction equivalent to 33% or 66% of food restriction. Fifteen days later, groups of 7 animals were injected with complete Freund's adjuvant plus spinal cord homogenate (SCH) to induce experimental allergic encephalomyelitis (EAE) or...

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Autores principales: Esquifino, Ana I, Cano, Pilar, Jimenez-Ortega, Vanessa, Fernández-Mateos, María P, Cardinali, Daniel P
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1793957/
https://www.ncbi.nlm.nih.gov/pubmed/17254325
http://dx.doi.org/10.1186/1742-2094-4-6
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author Esquifino, Ana I
Cano, Pilar
Jimenez-Ortega, Vanessa
Fernández-Mateos, María P
Cardinali, Daniel P
author_facet Esquifino, Ana I
Cano, Pilar
Jimenez-Ortega, Vanessa
Fernández-Mateos, María P
Cardinali, Daniel P
author_sort Esquifino, Ana I
collection PubMed
description Male Lewis rats (6 weeks-old) were submitted to a calorie restriction equivalent to 33% or 66% of food restriction. Fifteen days later, groups of 7 animals were injected with complete Freund's adjuvant plus spinal cord homogenate (SCH) to induce experimental allergic encephalomyelitis (EAE) or with complete Freund's adjuvant alone. EAE was defined solely on clinical grounds. Rats were assessed daily for clinical signs of EAE and were killed on day 15 after immunization. Both diet and SCH injection diminished body weight significantly. In contrast to rats receiving a normal diet or a 33% calorie-restricted diet, rats subjected to severe calorie restriction did not exhibit clinical signs of EAE. Concomitantly with the lack of disease manifestation, 66% of calorie-restricted rats injected with SCH showed significantly less splenic and lymph node mitogenic response to concanavalin A (Con A) and a higher splenic response to lipopolysaccharide. Fewer splenic, lymph node and thymic CD4(+ )cells, greater numbers of splenic and lymph node CD8(+ )and CD4(+)- CD8(+ )cells, and fewer splenic T, B and T-B cells, and lymph node and thymic B and T-B cells were observed. There was impaired interferon (IFN)-γ production occurred in the three examined tissues. The results are compatible with the view that the acute phase of EAE can be curtailed by severe calorie restriction, presumably through impaired IFN-γ production.
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spelling pubmed-17939572007-02-06 Immune response after experimental allergic encephalomyelitis in rats subjected to calorie restriction Esquifino, Ana I Cano, Pilar Jimenez-Ortega, Vanessa Fernández-Mateos, María P Cardinali, Daniel P J Neuroinflammation Research Male Lewis rats (6 weeks-old) were submitted to a calorie restriction equivalent to 33% or 66% of food restriction. Fifteen days later, groups of 7 animals were injected with complete Freund's adjuvant plus spinal cord homogenate (SCH) to induce experimental allergic encephalomyelitis (EAE) or with complete Freund's adjuvant alone. EAE was defined solely on clinical grounds. Rats were assessed daily for clinical signs of EAE and were killed on day 15 after immunization. Both diet and SCH injection diminished body weight significantly. In contrast to rats receiving a normal diet or a 33% calorie-restricted diet, rats subjected to severe calorie restriction did not exhibit clinical signs of EAE. Concomitantly with the lack of disease manifestation, 66% of calorie-restricted rats injected with SCH showed significantly less splenic and lymph node mitogenic response to concanavalin A (Con A) and a higher splenic response to lipopolysaccharide. Fewer splenic, lymph node and thymic CD4(+ )cells, greater numbers of splenic and lymph node CD8(+ )and CD4(+)- CD8(+ )cells, and fewer splenic T, B and T-B cells, and lymph node and thymic B and T-B cells were observed. There was impaired interferon (IFN)-γ production occurred in the three examined tissues. The results are compatible with the view that the acute phase of EAE can be curtailed by severe calorie restriction, presumably through impaired IFN-γ production. BioMed Central 2007-01-25 /pmc/articles/PMC1793957/ /pubmed/17254325 http://dx.doi.org/10.1186/1742-2094-4-6 Text en Copyright © 2007 Esquifino et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Esquifino, Ana I
Cano, Pilar
Jimenez-Ortega, Vanessa
Fernández-Mateos, María P
Cardinali, Daniel P
Immune response after experimental allergic encephalomyelitis in rats subjected to calorie restriction
title Immune response after experimental allergic encephalomyelitis in rats subjected to calorie restriction
title_full Immune response after experimental allergic encephalomyelitis in rats subjected to calorie restriction
title_fullStr Immune response after experimental allergic encephalomyelitis in rats subjected to calorie restriction
title_full_unstemmed Immune response after experimental allergic encephalomyelitis in rats subjected to calorie restriction
title_short Immune response after experimental allergic encephalomyelitis in rats subjected to calorie restriction
title_sort immune response after experimental allergic encephalomyelitis in rats subjected to calorie restriction
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1793957/
https://www.ncbi.nlm.nih.gov/pubmed/17254325
http://dx.doi.org/10.1186/1742-2094-4-6
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