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EGF increases expression and activity of PAs in preimplantation rat embryos and their implantation rate

BACKGROUND: Embryo implantation plays a major role in embryogenesis and the outcome of pregnancy. Plasminogen activators (PAs) have been implicated in mammalian fertilization, early stages of development and embryo implantation. As in-vitro developing embryos resulted in lower implantation rate than...

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Autores principales: Aflalo, Eliahu D, Sod-Moriah, Uriel A, Potashnik, Gad, Har-Vardi, Iris
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1794411/
https://www.ncbi.nlm.nih.gov/pubmed/17261179
http://dx.doi.org/10.1186/1477-7827-5-4
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author Aflalo, Eliahu D
Sod-Moriah, Uriel A
Potashnik, Gad
Har-Vardi, Iris
author_facet Aflalo, Eliahu D
Sod-Moriah, Uriel A
Potashnik, Gad
Har-Vardi, Iris
author_sort Aflalo, Eliahu D
collection PubMed
description BACKGROUND: Embryo implantation plays a major role in embryogenesis and the outcome of pregnancy. Plasminogen activators (PAs) have been implicated in mammalian fertilization, early stages of development and embryo implantation. As in-vitro developing embryos resulted in lower implantation rate than those developed in-vivo we assume that a reduced PAs activity may be involved. In the present work we studied the effect of EGF on PAs activity, quantity and embryo implantation. METHODS: Zygotes were flushed from rat oviducts on day one of pregnancy and grown in-vitro in R1ECM supplemented with EGF (10 ng/ml) and were grown up to the blastocyst stage. The control groups were grown in the same medium without EGF. The distribution and quantity of the PAs were examined using fluorescence immunohistochemistry followed by measurement of PAs activity using the chromogenic assay. Implantation rate was studied using the embryo donation model. RESULTS: PAs distribution in the embryos was the same in EGF treated and untreated embryos. Both PAs were localized in the blastocysts' trophectoderm, supporting the assumption that PAs play a role in the implantation process in rats. EGF increased the quantity of uPA at all stages studied but the 8-cell stage as compared with controls. The tissue type PA (tPA) content was unaffected except the 8-cell stage, which was increased. The activity of uPA increased gradually towards the blastocyst stage and more so due to the presence of EGF. The activity of tPA did not vary with the advancing developmental stages although it was also increased by EGF. The presence of EGF during the preimplantation development doubled the rate of implantation of the treated group as compared with controls.
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spelling pubmed-17944112007-02-08 EGF increases expression and activity of PAs in preimplantation rat embryos and their implantation rate Aflalo, Eliahu D Sod-Moriah, Uriel A Potashnik, Gad Har-Vardi, Iris Reprod Biol Endocrinol Research BACKGROUND: Embryo implantation plays a major role in embryogenesis and the outcome of pregnancy. Plasminogen activators (PAs) have been implicated in mammalian fertilization, early stages of development and embryo implantation. As in-vitro developing embryos resulted in lower implantation rate than those developed in-vivo we assume that a reduced PAs activity may be involved. In the present work we studied the effect of EGF on PAs activity, quantity and embryo implantation. METHODS: Zygotes were flushed from rat oviducts on day one of pregnancy and grown in-vitro in R1ECM supplemented with EGF (10 ng/ml) and were grown up to the blastocyst stage. The control groups were grown in the same medium without EGF. The distribution and quantity of the PAs were examined using fluorescence immunohistochemistry followed by measurement of PAs activity using the chromogenic assay. Implantation rate was studied using the embryo donation model. RESULTS: PAs distribution in the embryos was the same in EGF treated and untreated embryos. Both PAs were localized in the blastocysts' trophectoderm, supporting the assumption that PAs play a role in the implantation process in rats. EGF increased the quantity of uPA at all stages studied but the 8-cell stage as compared with controls. The tissue type PA (tPA) content was unaffected except the 8-cell stage, which was increased. The activity of uPA increased gradually towards the blastocyst stage and more so due to the presence of EGF. The activity of tPA did not vary with the advancing developmental stages although it was also increased by EGF. The presence of EGF during the preimplantation development doubled the rate of implantation of the treated group as compared with controls. BioMed Central 2007-01-29 /pmc/articles/PMC1794411/ /pubmed/17261179 http://dx.doi.org/10.1186/1477-7827-5-4 Text en Copyright © 2007 Aflalo et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Aflalo, Eliahu D
Sod-Moriah, Uriel A
Potashnik, Gad
Har-Vardi, Iris
EGF increases expression and activity of PAs in preimplantation rat embryos and their implantation rate
title EGF increases expression and activity of PAs in preimplantation rat embryos and their implantation rate
title_full EGF increases expression and activity of PAs in preimplantation rat embryos and their implantation rate
title_fullStr EGF increases expression and activity of PAs in preimplantation rat embryos and their implantation rate
title_full_unstemmed EGF increases expression and activity of PAs in preimplantation rat embryos and their implantation rate
title_short EGF increases expression and activity of PAs in preimplantation rat embryos and their implantation rate
title_sort egf increases expression and activity of pas in preimplantation rat embryos and their implantation rate
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1794411/
https://www.ncbi.nlm.nih.gov/pubmed/17261179
http://dx.doi.org/10.1186/1477-7827-5-4
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