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Generation of medaka gene knockout models by target-selected mutagenesis
We have established a reverse genetics approach for the routine generation of medaka (Oryzias latipes) gene knockouts. A cryopreserved library of N-ethyl-N-nitrosourea (ENU) mutagenized fish was screened by high-throughput resequencing for induced point mutations. Nonsense and splice site mutations...
| Autores principales: | , , , , , , , , , , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2006
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1794429/ https://www.ncbi.nlm.nih.gov/pubmed/17156454 http://dx.doi.org/10.1186/gb-2006-7-12-r116 |
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| author | Taniguchi, Yoshihito Takeda, Shunichi Furutani-Seiki, Makoto Kamei, Yasuhiro Todo, Takeshi Sasado, Takao Deguchi, Tomonori Kondoh, Hisato Mudde, Josine Yamazoe, Mitsuyoshi Hidaka, Masayuki Mitani, Hiroshi Toyoda, Atsushi Sakaki, Yoshiyuki Plasterk, Ronald HA Cuppen, Edwin |
| author_facet | Taniguchi, Yoshihito Takeda, Shunichi Furutani-Seiki, Makoto Kamei, Yasuhiro Todo, Takeshi Sasado, Takao Deguchi, Tomonori Kondoh, Hisato Mudde, Josine Yamazoe, Mitsuyoshi Hidaka, Masayuki Mitani, Hiroshi Toyoda, Atsushi Sakaki, Yoshiyuki Plasterk, Ronald HA Cuppen, Edwin |
| author_sort | Taniguchi, Yoshihito |
| collection | PubMed |
| description | We have established a reverse genetics approach for the routine generation of medaka (Oryzias latipes) gene knockouts. A cryopreserved library of N-ethyl-N-nitrosourea (ENU) mutagenized fish was screened by high-throughput resequencing for induced point mutations. Nonsense and splice site mutations were retrieved for the Blm, Sirt1, Parkin and p53 genes and functional characterization of p53 mutants indicated a complete knockout of p53 function. The current cryopreserved resource is expected to contain knockouts for most medaka genes. |
| format | Text |
| id | pubmed-1794429 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2006 |
| publisher | BioMed Central |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-17944292007-02-08 Generation of medaka gene knockout models by target-selected mutagenesis Taniguchi, Yoshihito Takeda, Shunichi Furutani-Seiki, Makoto Kamei, Yasuhiro Todo, Takeshi Sasado, Takao Deguchi, Tomonori Kondoh, Hisato Mudde, Josine Yamazoe, Mitsuyoshi Hidaka, Masayuki Mitani, Hiroshi Toyoda, Atsushi Sakaki, Yoshiyuki Plasterk, Ronald HA Cuppen, Edwin Genome Biol Method We have established a reverse genetics approach for the routine generation of medaka (Oryzias latipes) gene knockouts. A cryopreserved library of N-ethyl-N-nitrosourea (ENU) mutagenized fish was screened by high-throughput resequencing for induced point mutations. Nonsense and splice site mutations were retrieved for the Blm, Sirt1, Parkin and p53 genes and functional characterization of p53 mutants indicated a complete knockout of p53 function. The current cryopreserved resource is expected to contain knockouts for most medaka genes. BioMed Central 2006 2006-12-08 /pmc/articles/PMC1794429/ /pubmed/17156454 http://dx.doi.org/10.1186/gb-2006-7-12-r116 Text en Copyright © 2006 Taniguchi et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| spellingShingle | Method Taniguchi, Yoshihito Takeda, Shunichi Furutani-Seiki, Makoto Kamei, Yasuhiro Todo, Takeshi Sasado, Takao Deguchi, Tomonori Kondoh, Hisato Mudde, Josine Yamazoe, Mitsuyoshi Hidaka, Masayuki Mitani, Hiroshi Toyoda, Atsushi Sakaki, Yoshiyuki Plasterk, Ronald HA Cuppen, Edwin Generation of medaka gene knockout models by target-selected mutagenesis |
| title | Generation of medaka gene knockout models by target-selected mutagenesis |
| title_full | Generation of medaka gene knockout models by target-selected mutagenesis |
| title_fullStr | Generation of medaka gene knockout models by target-selected mutagenesis |
| title_full_unstemmed | Generation of medaka gene knockout models by target-selected mutagenesis |
| title_short | Generation of medaka gene knockout models by target-selected mutagenesis |
| title_sort | generation of medaka gene knockout models by target-selected mutagenesis |
| topic | Method |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1794429/ https://www.ncbi.nlm.nih.gov/pubmed/17156454 http://dx.doi.org/10.1186/gb-2006-7-12-r116 |
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