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Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reaction

The molecular basis to mammalian osteoarthritis (OA) is unknown. We hypothesised that the expression of selected proteases, matrix molecules, and collagens believed to have a role in the pathogenesis of OA would be changed in naturally occurring canine OA cartilage when compared to normal articular...

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Autores principales: Clements, Dylan N, Carter, Stuart D, Innes, John F, Ollier, William ER, Day, Philip JR
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1794499/
https://www.ncbi.nlm.nih.gov/pubmed/17032449
http://dx.doi.org/10.1186/ar2053
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author Clements, Dylan N
Carter, Stuart D
Innes, John F
Ollier, William ER
Day, Philip JR
author_facet Clements, Dylan N
Carter, Stuart D
Innes, John F
Ollier, William ER
Day, Philip JR
author_sort Clements, Dylan N
collection PubMed
description The molecular basis to mammalian osteoarthritis (OA) is unknown. We hypothesised that the expression of selected proteases, matrix molecules, and collagens believed to have a role in the pathogenesis of OA would be changed in naturally occurring canine OA cartilage when compared to normal articular cartilage. Quantitative (real-time) reverse transcriptase-polymerase chain reaction assays were designed measuring the expression of selected matrix molecules (collagens and small leucine-rich proteoglycans), key mediators of the proteolytic degradation of articular cartilage (metalloproteinases, cathepsins), and their inhibitors (tissue inhibitors of matrix metalloproteinases). All data were normalised using a geometric mean of three housekeeping genes, and the results subjected to power calculations and corrections for multiple hypothesis testing. We detected increases in the expression of BGN, COL1A2, COL2A1, COL3A1, COL5A1, CSPG2, CTSB, CTSD, LUM, MMP13, TIMP1, and TNC in naturally occurring canine OA. The expression of TIMP2 and TIMP4 was significantly reduced in canine OA cartilage. The patterns of gene expression change observed in naturally occurring canine OA were similar to those reported in naturally occurring human OA and experimental canine OA. We conclude that the expression profiles of matrix-associated molecules in end-stage mammalian OA may be comparable but that the precise aetiologies of OA affecting specific joints in different species are presently unknown.
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spelling pubmed-17944992007-02-08 Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reaction Clements, Dylan N Carter, Stuart D Innes, John F Ollier, William ER Day, Philip JR Arthritis Res Ther Research Article The molecular basis to mammalian osteoarthritis (OA) is unknown. We hypothesised that the expression of selected proteases, matrix molecules, and collagens believed to have a role in the pathogenesis of OA would be changed in naturally occurring canine OA cartilage when compared to normal articular cartilage. Quantitative (real-time) reverse transcriptase-polymerase chain reaction assays were designed measuring the expression of selected matrix molecules (collagens and small leucine-rich proteoglycans), key mediators of the proteolytic degradation of articular cartilage (metalloproteinases, cathepsins), and their inhibitors (tissue inhibitors of matrix metalloproteinases). All data were normalised using a geometric mean of three housekeeping genes, and the results subjected to power calculations and corrections for multiple hypothesis testing. We detected increases in the expression of BGN, COL1A2, COL2A1, COL3A1, COL5A1, CSPG2, CTSB, CTSD, LUM, MMP13, TIMP1, and TNC in naturally occurring canine OA. The expression of TIMP2 and TIMP4 was significantly reduced in canine OA cartilage. The patterns of gene expression change observed in naturally occurring canine OA were similar to those reported in naturally occurring human OA and experimental canine OA. We conclude that the expression profiles of matrix-associated molecules in end-stage mammalian OA may be comparable but that the precise aetiologies of OA affecting specific joints in different species are presently unknown. BioMed Central 2006 2006-10-10 /pmc/articles/PMC1794499/ /pubmed/17032449 http://dx.doi.org/10.1186/ar2053 Text en Copyright © 2006 Clements et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Clements, Dylan N
Carter, Stuart D
Innes, John F
Ollier, William ER
Day, Philip JR
Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reaction
title Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reaction
title_full Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reaction
title_fullStr Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reaction
title_full_unstemmed Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reaction
title_short Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reaction
title_sort analysis of normal and osteoarthritic canine cartilage mrna expression by quantitative polymerase chain reaction
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1794499/
https://www.ncbi.nlm.nih.gov/pubmed/17032449
http://dx.doi.org/10.1186/ar2053
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