Relating a calcium indicator signal to the unperturbed calcium concentration time-course

BACKGROUND: Optical indicators of cytosolic calcium levels have become important experimental tools in systems and cellular neuroscience. Indicators are known to interfere with intracellular calcium levels by acting as additional buffers, and this may strongly alter the time-course of various dynami...

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Detalles Bibliográficos
Autores principales: Borst, Alexander, Abarbanel, Henry DI
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1800305/
https://www.ncbi.nlm.nih.gov/pubmed/17284310
http://dx.doi.org/10.1186/1742-4682-4-7
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author Borst, Alexander
Abarbanel, Henry DI
author_facet Borst, Alexander
Abarbanel, Henry DI
author_sort Borst, Alexander
collection PubMed
description BACKGROUND: Optical indicators of cytosolic calcium levels have become important experimental tools in systems and cellular neuroscience. Indicators are known to interfere with intracellular calcium levels by acting as additional buffers, and this may strongly alter the time-course of various dynamical variables to be measured. RESULTS: By investigating the underlying reaction kinetics, we show that in some ranges of kinetic parameters one can explicitly link the time dependent indicator signal to the time-course of the calcium influx, and thus, to the unperturbed calcium level had there been no indicator in the cell.
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spelling pubmed-18003052007-02-16 Relating a calcium indicator signal to the unperturbed calcium concentration time-course Borst, Alexander Abarbanel, Henry DI Theor Biol Med Model Research BACKGROUND: Optical indicators of cytosolic calcium levels have become important experimental tools in systems and cellular neuroscience. Indicators are known to interfere with intracellular calcium levels by acting as additional buffers, and this may strongly alter the time-course of various dynamical variables to be measured. RESULTS: By investigating the underlying reaction kinetics, we show that in some ranges of kinetic parameters one can explicitly link the time dependent indicator signal to the time-course of the calcium influx, and thus, to the unperturbed calcium level had there been no indicator in the cell. BioMed Central 2007-02-06 /pmc/articles/PMC1800305/ /pubmed/17284310 http://dx.doi.org/10.1186/1742-4682-4-7 Text en Copyright © 2007 Borst and Abarbanel; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Borst, Alexander
Abarbanel, Henry DI
Relating a calcium indicator signal to the unperturbed calcium concentration time-course
title Relating a calcium indicator signal to the unperturbed calcium concentration time-course
title_full Relating a calcium indicator signal to the unperturbed calcium concentration time-course
title_fullStr Relating a calcium indicator signal to the unperturbed calcium concentration time-course
title_full_unstemmed Relating a calcium indicator signal to the unperturbed calcium concentration time-course
title_short Relating a calcium indicator signal to the unperturbed calcium concentration time-course
title_sort relating a calcium indicator signal to the unperturbed calcium concentration time-course
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1800305/
https://www.ncbi.nlm.nih.gov/pubmed/17284310
http://dx.doi.org/10.1186/1742-4682-4-7
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