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No detectable effect of RNA-binding protein Hfq absence in Staphylococcus aureus

BACKGROUND: The RNA-binding protein Hfq is involved in stress and virulence of several pathogens, probably due to its role as mediator in small RNA (sRNA)-mRNA interactions. In this study, we investigate the function of Hfq in the Gram-positive pathogen Staphylococcus aureus, by constructing hfq nul...

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Autores principales: Bohn, Chantal, Rigoulay, Candice, Bouloc, Philippe
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1800855/
https://www.ncbi.nlm.nih.gov/pubmed/17291347
http://dx.doi.org/10.1186/1471-2180-7-10
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author Bohn, Chantal
Rigoulay, Candice
Bouloc, Philippe
author_facet Bohn, Chantal
Rigoulay, Candice
Bouloc, Philippe
author_sort Bohn, Chantal
collection PubMed
description BACKGROUND: The RNA-binding protein Hfq is involved in stress and virulence of several pathogens, probably due to its role as mediator in small RNA (sRNA)-mRNA interactions. In this study, we investigate the function of Hfq in the Gram-positive pathogen Staphylococcus aureus, by constructing hfq null mutant derivatives. RESULTS: We report that unexpectedly, in S. aureus, Hfq does not seem to play a crucial role in stress response, RNAIII or spa mRNA quantity and exoprotein expression, as tested in three virulent genetic backgrounds. Moreover, a global analysis of the RN6390 hfq mutant, which tests ~ 2000 phenotypes, supports our results concerning the non-implication of Hfq in stress response, and shows that Hfq is also not involved in resistance to several chemical agents and antibiotics and does not seem to be implicated in metabolic pathways. CONCLUSION: Our data suggest that although sRNA-mRNA interactions in S. aureus are decisive for gene expression regulation, they do not require the RNA-chaperone protein Hfq. These interactions possibly require an RNA-chaperone protein other than Hfq, which remains to be found.
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spelling pubmed-18008552007-02-17 No detectable effect of RNA-binding protein Hfq absence in Staphylococcus aureus Bohn, Chantal Rigoulay, Candice Bouloc, Philippe BMC Microbiol Research Article BACKGROUND: The RNA-binding protein Hfq is involved in stress and virulence of several pathogens, probably due to its role as mediator in small RNA (sRNA)-mRNA interactions. In this study, we investigate the function of Hfq in the Gram-positive pathogen Staphylococcus aureus, by constructing hfq null mutant derivatives. RESULTS: We report that unexpectedly, in S. aureus, Hfq does not seem to play a crucial role in stress response, RNAIII or spa mRNA quantity and exoprotein expression, as tested in three virulent genetic backgrounds. Moreover, a global analysis of the RN6390 hfq mutant, which tests ~ 2000 phenotypes, supports our results concerning the non-implication of Hfq in stress response, and shows that Hfq is also not involved in resistance to several chemical agents and antibiotics and does not seem to be implicated in metabolic pathways. CONCLUSION: Our data suggest that although sRNA-mRNA interactions in S. aureus are decisive for gene expression regulation, they do not require the RNA-chaperone protein Hfq. These interactions possibly require an RNA-chaperone protein other than Hfq, which remains to be found. BioMed Central 2007-02-09 /pmc/articles/PMC1800855/ /pubmed/17291347 http://dx.doi.org/10.1186/1471-2180-7-10 Text en Copyright © 2007 Bohn et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bohn, Chantal
Rigoulay, Candice
Bouloc, Philippe
No detectable effect of RNA-binding protein Hfq absence in Staphylococcus aureus
title No detectable effect of RNA-binding protein Hfq absence in Staphylococcus aureus
title_full No detectable effect of RNA-binding protein Hfq absence in Staphylococcus aureus
title_fullStr No detectable effect of RNA-binding protein Hfq absence in Staphylococcus aureus
title_full_unstemmed No detectable effect of RNA-binding protein Hfq absence in Staphylococcus aureus
title_short No detectable effect of RNA-binding protein Hfq absence in Staphylococcus aureus
title_sort no detectable effect of rna-binding protein hfq absence in staphylococcus aureus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1800855/
https://www.ncbi.nlm.nih.gov/pubmed/17291347
http://dx.doi.org/10.1186/1471-2180-7-10
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