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Functional interaction between the homeoprotein CDX1 and the transcriptional machinery containing the TATA-binding protein

We have previously reported that the CDX1 homeoprotein interacts with the TATA-box binding protein (TBP) on the promoter of the glucose-6-phosphatase (G6Pase) gene. We show here that CDX1 interacts with TBP via the homeodomain and that the transcriptional activity additionally requires the N-termina...

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Autores principales: Calon, Alexandre, Gross, Isabelle, Davidson, Irwin, Kedinger, Michèle, Duluc, Isabelle, Domon-Dell, Claire, Freund, Jean-Noël
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1802564/
https://www.ncbi.nlm.nih.gov/pubmed/17158164
http://dx.doi.org/10.1093/nar/gkl1034
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author Calon, Alexandre
Gross, Isabelle
Davidson, Irwin
Kedinger, Michèle
Duluc, Isabelle
Domon-Dell, Claire
Freund, Jean-Noël
author_facet Calon, Alexandre
Gross, Isabelle
Davidson, Irwin
Kedinger, Michèle
Duluc, Isabelle
Domon-Dell, Claire
Freund, Jean-Noël
author_sort Calon, Alexandre
collection PubMed
description We have previously reported that the CDX1 homeoprotein interacts with the TATA-box binding protein (TBP) on the promoter of the glucose-6-phosphatase (G6Pase) gene. We show here that CDX1 interacts with TBP via the homeodomain and that the transcriptional activity additionally requires the N-terminal domain upstream of the homeodomain. CDX1 interacting with TBP is connected to members of the TFIID and Mediator complexes, two major elements of the general transcriptional machinery. Transcription luciferase assays performed using an altered-specificity mutant of TBP provide evidence for the functionality of the interaction between CDX1 and TBP. Unlike CDX1, CDX2 does not interact with TBP nor does it transactivate the G6Pase promoter. Swapping experiments between the domains of CDX1 and CDX2 indicate that, despite opposite functional effects of the homeoproteins on the G6Pase promoter, the N-terminal domains and homeodomains of both CDX1 and CDX2 have the intrinsic ability to activate transcription and to interact with TBP. However, the carboxy domains define the specificity of CDX1 and CDX2. Thus, intra-molecular interactions control the activity and partner recruitment of CDX1 and CDX2, leading to different molecular functions.
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spelling pubmed-18025642007-03-01 Functional interaction between the homeoprotein CDX1 and the transcriptional machinery containing the TATA-binding protein Calon, Alexandre Gross, Isabelle Davidson, Irwin Kedinger, Michèle Duluc, Isabelle Domon-Dell, Claire Freund, Jean-Noël Nucleic Acids Res Molecular Biology We have previously reported that the CDX1 homeoprotein interacts with the TATA-box binding protein (TBP) on the promoter of the glucose-6-phosphatase (G6Pase) gene. We show here that CDX1 interacts with TBP via the homeodomain and that the transcriptional activity additionally requires the N-terminal domain upstream of the homeodomain. CDX1 interacting with TBP is connected to members of the TFIID and Mediator complexes, two major elements of the general transcriptional machinery. Transcription luciferase assays performed using an altered-specificity mutant of TBP provide evidence for the functionality of the interaction between CDX1 and TBP. Unlike CDX1, CDX2 does not interact with TBP nor does it transactivate the G6Pase promoter. Swapping experiments between the domains of CDX1 and CDX2 indicate that, despite opposite functional effects of the homeoproteins on the G6Pase promoter, the N-terminal domains and homeodomains of both CDX1 and CDX2 have the intrinsic ability to activate transcription and to interact with TBP. However, the carboxy domains define the specificity of CDX1 and CDX2. Thus, intra-molecular interactions control the activity and partner recruitment of CDX1 and CDX2, leading to different molecular functions. Oxford University Press 2007-01 2006-12-07 /pmc/articles/PMC1802564/ /pubmed/17158164 http://dx.doi.org/10.1093/nar/gkl1034 Text en © 2006 The Author(s) This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Molecular Biology
Calon, Alexandre
Gross, Isabelle
Davidson, Irwin
Kedinger, Michèle
Duluc, Isabelle
Domon-Dell, Claire
Freund, Jean-Noël
Functional interaction between the homeoprotein CDX1 and the transcriptional machinery containing the TATA-binding protein
title Functional interaction between the homeoprotein CDX1 and the transcriptional machinery containing the TATA-binding protein
title_full Functional interaction between the homeoprotein CDX1 and the transcriptional machinery containing the TATA-binding protein
title_fullStr Functional interaction between the homeoprotein CDX1 and the transcriptional machinery containing the TATA-binding protein
title_full_unstemmed Functional interaction between the homeoprotein CDX1 and the transcriptional machinery containing the TATA-binding protein
title_short Functional interaction between the homeoprotein CDX1 and the transcriptional machinery containing the TATA-binding protein
title_sort functional interaction between the homeoprotein cdx1 and the transcriptional machinery containing the tata-binding protein
topic Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1802564/
https://www.ncbi.nlm.nih.gov/pubmed/17158164
http://dx.doi.org/10.1093/nar/gkl1034
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