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Protein Buffering in Model Systems and in Whole Human Saliva

The aim of this study was to quantify the buffer attributes (value, power, range and optimum) of two model systems for whole human resting saliva, the purified proteins from whole human resting saliva and single proteins. Two model systems, the first containing amyloglucosidase and lysozyme, and the...

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Detalles Bibliográficos
Autores principales: Lamanda, Andreas, Cheaib, Zeinab, Turgut, Melek Dilek, Lussi, Adrian
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1803027/
https://www.ncbi.nlm.nih.gov/pubmed/17327922
http://dx.doi.org/10.1371/journal.pone.0000263
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author Lamanda, Andreas
Cheaib, Zeinab
Turgut, Melek Dilek
Lussi, Adrian
author_facet Lamanda, Andreas
Cheaib, Zeinab
Turgut, Melek Dilek
Lussi, Adrian
author_sort Lamanda, Andreas
collection PubMed
description The aim of this study was to quantify the buffer attributes (value, power, range and optimum) of two model systems for whole human resting saliva, the purified proteins from whole human resting saliva and single proteins. Two model systems, the first containing amyloglucosidase and lysozyme, and the second containing amyloglucosidase and α-amylase, were shown to provide, in combination with hydrogencarbonate and di-hydrogenphosphate, almost identical buffer attributes as whole human resting saliva. It was further demonstrated that changes in the protein concentration as small as 0.1% may change the buffer value of a buffer solution up to 15 times. Additionally, it was shown that there was a protein concentration change in the same range (0.16%) between saliva samples collected at the time periods of 13:00 and others collected at 9:00 am and 17:00. The mode of the protein expression changed between these samples corresponded to the change in basic buffer power and the change of the buffer value at pH 6.7. Finally, SDS Page and Ruthenium II tris (bathophenantroline disulfonate) staining unveiled a constant protein expression in all samples except for one 50 kDa protein band. As the change in the expression pattern of that 50 kDa protein band corresponded to the change in basic buffer power and the buffer value at pH 6.7, it was reasonable to conclude that this 50 kDa protein band may contain the protein(s) belonging to the protein buffer system of human saliva.
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spelling pubmed-18030272007-02-28 Protein Buffering in Model Systems and in Whole Human Saliva Lamanda, Andreas Cheaib, Zeinab Turgut, Melek Dilek Lussi, Adrian PLoS One Research Article The aim of this study was to quantify the buffer attributes (value, power, range and optimum) of two model systems for whole human resting saliva, the purified proteins from whole human resting saliva and single proteins. Two model systems, the first containing amyloglucosidase and lysozyme, and the second containing amyloglucosidase and α-amylase, were shown to provide, in combination with hydrogencarbonate and di-hydrogenphosphate, almost identical buffer attributes as whole human resting saliva. It was further demonstrated that changes in the protein concentration as small as 0.1% may change the buffer value of a buffer solution up to 15 times. Additionally, it was shown that there was a protein concentration change in the same range (0.16%) between saliva samples collected at the time periods of 13:00 and others collected at 9:00 am and 17:00. The mode of the protein expression changed between these samples corresponded to the change in basic buffer power and the change of the buffer value at pH 6.7. Finally, SDS Page and Ruthenium II tris (bathophenantroline disulfonate) staining unveiled a constant protein expression in all samples except for one 50 kDa protein band. As the change in the expression pattern of that 50 kDa protein band corresponded to the change in basic buffer power and the buffer value at pH 6.7, it was reasonable to conclude that this 50 kDa protein band may contain the protein(s) belonging to the protein buffer system of human saliva. Public Library of Science 2007-02-28 /pmc/articles/PMC1803027/ /pubmed/17327922 http://dx.doi.org/10.1371/journal.pone.0000263 Text en Lamanda et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Lamanda, Andreas
Cheaib, Zeinab
Turgut, Melek Dilek
Lussi, Adrian
Protein Buffering in Model Systems and in Whole Human Saliva
title Protein Buffering in Model Systems and in Whole Human Saliva
title_full Protein Buffering in Model Systems and in Whole Human Saliva
title_fullStr Protein Buffering in Model Systems and in Whole Human Saliva
title_full_unstemmed Protein Buffering in Model Systems and in Whole Human Saliva
title_short Protein Buffering in Model Systems and in Whole Human Saliva
title_sort protein buffering in model systems and in whole human saliva
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1803027/
https://www.ncbi.nlm.nih.gov/pubmed/17327922
http://dx.doi.org/10.1371/journal.pone.0000263
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