Cargando…

σ(E)-dependent small RNAs of Salmonella respond to membrane stress by accelerating global omp mRNA decay

The bacterial envelope stress response (ESR) is triggered by the accumulation of misfolded outer membrane proteins (OMPs) upon envelope damage or excessive OMP synthesis, and is mediated by the alternative sigma factor, σ(E). Activation of the σ(E) pathway causes a rapid downregulation of major omp...

Descripción completa

Detalles Bibliográficos
Autores principales: Papenfort, Kai, Pfeiffer, Verena, Mika, Franziska, Lucchini, Sacha, Hinton, Jay C D, Vogel, Jörg
Formato: Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1804206/
https://www.ncbi.nlm.nih.gov/pubmed/17427289
http://dx.doi.org/10.1111/j.1365-2958.2006.05524.x
_version_ 1782132440826904576
author Papenfort, Kai
Pfeiffer, Verena
Mika, Franziska
Lucchini, Sacha
Hinton, Jay C D
Vogel, Jörg
author_facet Papenfort, Kai
Pfeiffer, Verena
Mika, Franziska
Lucchini, Sacha
Hinton, Jay C D
Vogel, Jörg
author_sort Papenfort, Kai
collection PubMed
description The bacterial envelope stress response (ESR) is triggered by the accumulation of misfolded outer membrane proteins (OMPs) upon envelope damage or excessive OMP synthesis, and is mediated by the alternative sigma factor, σ(E). Activation of the σ(E) pathway causes a rapid downregulation of major omp mRNAs, which prevents further build-up of unassembled OMPs and liberates the translocation and folding apparatus under conditions that require envelope remodelling. The factors that facilitate the rapid removal of the unusually stable omp mRNAs in the ESR were previously unknown. We report that in Salmonella the ESR relies upon two highly conserved, σ(E)-controlled small non-coding RNAs, RybB and MicA. By using a transcriptomic approach and kinetic analyses of target mRNA decay in vivo, RybB was identified as the factor that selectively accelerates the decay of multiple major omp mRNAs upon induction of the ESR, while MicA is proposed to facilitate rapid decay of the single ompA mRNA. In unstressed bacterial cells, the two σ(E)-dependent small RNAs function within a surveillance loop to maintain envelope homeostasis and to achieve autoregulation of σ(E).
format Text
id pubmed-1804206
institution National Center for Biotechnology Information
language English
publishDate 2006
publisher Blackwell Publishing Ltd
record_format MEDLINE/PubMed
spelling pubmed-18042062007-03-06 σ(E)-dependent small RNAs of Salmonella respond to membrane stress by accelerating global omp mRNA decay Papenfort, Kai Pfeiffer, Verena Mika, Franziska Lucchini, Sacha Hinton, Jay C D Vogel, Jörg Mol Microbiol Research Articles The bacterial envelope stress response (ESR) is triggered by the accumulation of misfolded outer membrane proteins (OMPs) upon envelope damage or excessive OMP synthesis, and is mediated by the alternative sigma factor, σ(E). Activation of the σ(E) pathway causes a rapid downregulation of major omp mRNAs, which prevents further build-up of unassembled OMPs and liberates the translocation and folding apparatus under conditions that require envelope remodelling. The factors that facilitate the rapid removal of the unusually stable omp mRNAs in the ESR were previously unknown. We report that in Salmonella the ESR relies upon two highly conserved, σ(E)-controlled small non-coding RNAs, RybB and MicA. By using a transcriptomic approach and kinetic analyses of target mRNA decay in vivo, RybB was identified as the factor that selectively accelerates the decay of multiple major omp mRNAs upon induction of the ESR, while MicA is proposed to facilitate rapid decay of the single ompA mRNA. In unstressed bacterial cells, the two σ(E)-dependent small RNAs function within a surveillance loop to maintain envelope homeostasis and to achieve autoregulation of σ(E). Blackwell Publishing Ltd 2006-12 /pmc/articles/PMC1804206/ /pubmed/17427289 http://dx.doi.org/10.1111/j.1365-2958.2006.05524.x Text en © 2006 The Authors; Journal compilation © 2006 Blackwell Publishing Ltd https://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
spellingShingle Research Articles
Papenfort, Kai
Pfeiffer, Verena
Mika, Franziska
Lucchini, Sacha
Hinton, Jay C D
Vogel, Jörg
σ(E)-dependent small RNAs of Salmonella respond to membrane stress by accelerating global omp mRNA decay
title σ(E)-dependent small RNAs of Salmonella respond to membrane stress by accelerating global omp mRNA decay
title_full σ(E)-dependent small RNAs of Salmonella respond to membrane stress by accelerating global omp mRNA decay
title_fullStr σ(E)-dependent small RNAs of Salmonella respond to membrane stress by accelerating global omp mRNA decay
title_full_unstemmed σ(E)-dependent small RNAs of Salmonella respond to membrane stress by accelerating global omp mRNA decay
title_short σ(E)-dependent small RNAs of Salmonella respond to membrane stress by accelerating global omp mRNA decay
title_sort σ(e)-dependent small rnas of salmonella respond to membrane stress by accelerating global omp mrna decay
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1804206/
https://www.ncbi.nlm.nih.gov/pubmed/17427289
http://dx.doi.org/10.1111/j.1365-2958.2006.05524.x
work_keys_str_mv AT papenfortkai sedependentsmallrnasofsalmonellarespondtomembranestressbyacceleratingglobalompmrnadecay
AT pfeifferverena sedependentsmallrnasofsalmonellarespondtomembranestressbyacceleratingglobalompmrnadecay
AT mikafranziska sedependentsmallrnasofsalmonellarespondtomembranestressbyacceleratingglobalompmrnadecay
AT lucchinisacha sedependentsmallrnasofsalmonellarespondtomembranestressbyacceleratingglobalompmrnadecay
AT hintonjaycd sedependentsmallrnasofsalmonellarespondtomembranestressbyacceleratingglobalompmrnadecay
AT vogeljorg sedependentsmallrnasofsalmonellarespondtomembranestressbyacceleratingglobalompmrnadecay