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σ(E)-dependent small RNAs of Salmonella respond to membrane stress by accelerating global omp mRNA decay
The bacterial envelope stress response (ESR) is triggered by the accumulation of misfolded outer membrane proteins (OMPs) upon envelope damage or excessive OMP synthesis, and is mediated by the alternative sigma factor, σ(E). Activation of the σ(E) pathway causes a rapid downregulation of major omp...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1804206/ https://www.ncbi.nlm.nih.gov/pubmed/17427289 http://dx.doi.org/10.1111/j.1365-2958.2006.05524.x |
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author | Papenfort, Kai Pfeiffer, Verena Mika, Franziska Lucchini, Sacha Hinton, Jay C D Vogel, Jörg |
author_facet | Papenfort, Kai Pfeiffer, Verena Mika, Franziska Lucchini, Sacha Hinton, Jay C D Vogel, Jörg |
author_sort | Papenfort, Kai |
collection | PubMed |
description | The bacterial envelope stress response (ESR) is triggered by the accumulation of misfolded outer membrane proteins (OMPs) upon envelope damage or excessive OMP synthesis, and is mediated by the alternative sigma factor, σ(E). Activation of the σ(E) pathway causes a rapid downregulation of major omp mRNAs, which prevents further build-up of unassembled OMPs and liberates the translocation and folding apparatus under conditions that require envelope remodelling. The factors that facilitate the rapid removal of the unusually stable omp mRNAs in the ESR were previously unknown. We report that in Salmonella the ESR relies upon two highly conserved, σ(E)-controlled small non-coding RNAs, RybB and MicA. By using a transcriptomic approach and kinetic analyses of target mRNA decay in vivo, RybB was identified as the factor that selectively accelerates the decay of multiple major omp mRNAs upon induction of the ESR, while MicA is proposed to facilitate rapid decay of the single ompA mRNA. In unstressed bacterial cells, the two σ(E)-dependent small RNAs function within a surveillance loop to maintain envelope homeostasis and to achieve autoregulation of σ(E). |
format | Text |
id | pubmed-1804206 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-18042062007-03-06 σ(E)-dependent small RNAs of Salmonella respond to membrane stress by accelerating global omp mRNA decay Papenfort, Kai Pfeiffer, Verena Mika, Franziska Lucchini, Sacha Hinton, Jay C D Vogel, Jörg Mol Microbiol Research Articles The bacterial envelope stress response (ESR) is triggered by the accumulation of misfolded outer membrane proteins (OMPs) upon envelope damage or excessive OMP synthesis, and is mediated by the alternative sigma factor, σ(E). Activation of the σ(E) pathway causes a rapid downregulation of major omp mRNAs, which prevents further build-up of unassembled OMPs and liberates the translocation and folding apparatus under conditions that require envelope remodelling. The factors that facilitate the rapid removal of the unusually stable omp mRNAs in the ESR were previously unknown. We report that in Salmonella the ESR relies upon two highly conserved, σ(E)-controlled small non-coding RNAs, RybB and MicA. By using a transcriptomic approach and kinetic analyses of target mRNA decay in vivo, RybB was identified as the factor that selectively accelerates the decay of multiple major omp mRNAs upon induction of the ESR, while MicA is proposed to facilitate rapid decay of the single ompA mRNA. In unstressed bacterial cells, the two σ(E)-dependent small RNAs function within a surveillance loop to maintain envelope homeostasis and to achieve autoregulation of σ(E). Blackwell Publishing Ltd 2006-12 /pmc/articles/PMC1804206/ /pubmed/17427289 http://dx.doi.org/10.1111/j.1365-2958.2006.05524.x Text en © 2006 The Authors; Journal compilation © 2006 Blackwell Publishing Ltd https://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation. |
spellingShingle | Research Articles Papenfort, Kai Pfeiffer, Verena Mika, Franziska Lucchini, Sacha Hinton, Jay C D Vogel, Jörg σ(E)-dependent small RNAs of Salmonella respond to membrane stress by accelerating global omp mRNA decay |
title | σ(E)-dependent small RNAs of Salmonella respond to membrane stress by accelerating global omp mRNA decay |
title_full | σ(E)-dependent small RNAs of Salmonella respond to membrane stress by accelerating global omp mRNA decay |
title_fullStr | σ(E)-dependent small RNAs of Salmonella respond to membrane stress by accelerating global omp mRNA decay |
title_full_unstemmed | σ(E)-dependent small RNAs of Salmonella respond to membrane stress by accelerating global omp mRNA decay |
title_short | σ(E)-dependent small RNAs of Salmonella respond to membrane stress by accelerating global omp mRNA decay |
title_sort | σ(e)-dependent small rnas of salmonella respond to membrane stress by accelerating global omp mrna decay |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1804206/ https://www.ncbi.nlm.nih.gov/pubmed/17427289 http://dx.doi.org/10.1111/j.1365-2958.2006.05524.x |
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