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The testis and epididymis are productively infected by SIV and SHIV in juvenile macaques during the post-acute stage of infection

BACKGROUND: Little is known about the progression and pathogenesis of HIV-1 infection within the male genital tract (MGT), particularly during the early stages of infection. RESULTS: To study HIV pathogenesis in the testis and epididymis, 12 juvenile monkeys (Macacca nemestrina, 4–4.5 years old) wer...

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Autores principales: Shehu-Xhilaga, Miranda, Kent, Stephen, Batten, Jane, Ellis, Sarah, Van der Meulen, Joel, O'Bryan, Moira, Cameron, Paul U, Lewin, Sharon R, Hedger, Mark P
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1805449/
https://www.ncbi.nlm.nih.gov/pubmed/17266752
http://dx.doi.org/10.1186/1742-4690-4-7
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author Shehu-Xhilaga, Miranda
Kent, Stephen
Batten, Jane
Ellis, Sarah
Van der Meulen, Joel
O'Bryan, Moira
Cameron, Paul U
Lewin, Sharon R
Hedger, Mark P
author_facet Shehu-Xhilaga, Miranda
Kent, Stephen
Batten, Jane
Ellis, Sarah
Van der Meulen, Joel
O'Bryan, Moira
Cameron, Paul U
Lewin, Sharon R
Hedger, Mark P
author_sort Shehu-Xhilaga, Miranda
collection PubMed
description BACKGROUND: Little is known about the progression and pathogenesis of HIV-1 infection within the male genital tract (MGT), particularly during the early stages of infection. RESULTS: To study HIV pathogenesis in the testis and epididymis, 12 juvenile monkeys (Macacca nemestrina, 4–4.5 years old) were infected with Simian Immunodeficiency Virus mac 251 (SIV(mac251)) (n = 6) or Simian/Human Immunodeficiency Virus (SHIV(mn229)) (n = 6). Testes and epididymides were collected and examined by light microscopy and electron microscopy, at weeks 11–13 (SHIV) and 23 (SIV) following infection. Differences were found in the maturation status of the MGT of the monkeys, ranging from prepubertal (lacking post-meiotic germ cells) to post-pubertal (having mature sperm in the epididymal duct). Variable levels of viral RNA were identified in the lymph node, epididymis and testis following infection with both SHIV(mn229 )and SIV(mac251). Viral protein was detected via immunofluorescence histochemistry using specific antibodies to SIV (anti-gp41) and HIV-1 (capsid/p24) protein. SIV and SHIV infected macrophages, potentially dendritic cells and T cells in the testicular interstitial tissue were identified by co-localisation studies using antibodies to CD68, DC-SIGN, αβTCR. Infection of spermatogonia, but not more mature spermatogenic cells, was also observed. Leukocytic infiltrates were observed within the epididymal stroma of the infected animals. CONCLUSION: These data show that the testis and epididymis of juvenile macaques are a target for SIV and SHIV during the post-acute stage of infection and represent a potential model for studying HIV-1 pathogenesis and its effect on spermatogenesis and the MGT in general.
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spelling pubmed-18054492007-02-27 The testis and epididymis are productively infected by SIV and SHIV in juvenile macaques during the post-acute stage of infection Shehu-Xhilaga, Miranda Kent, Stephen Batten, Jane Ellis, Sarah Van der Meulen, Joel O'Bryan, Moira Cameron, Paul U Lewin, Sharon R Hedger, Mark P Retrovirology Research BACKGROUND: Little is known about the progression and pathogenesis of HIV-1 infection within the male genital tract (MGT), particularly during the early stages of infection. RESULTS: To study HIV pathogenesis in the testis and epididymis, 12 juvenile monkeys (Macacca nemestrina, 4–4.5 years old) were infected with Simian Immunodeficiency Virus mac 251 (SIV(mac251)) (n = 6) or Simian/Human Immunodeficiency Virus (SHIV(mn229)) (n = 6). Testes and epididymides were collected and examined by light microscopy and electron microscopy, at weeks 11–13 (SHIV) and 23 (SIV) following infection. Differences were found in the maturation status of the MGT of the monkeys, ranging from prepubertal (lacking post-meiotic germ cells) to post-pubertal (having mature sperm in the epididymal duct). Variable levels of viral RNA were identified in the lymph node, epididymis and testis following infection with both SHIV(mn229 )and SIV(mac251). Viral protein was detected via immunofluorescence histochemistry using specific antibodies to SIV (anti-gp41) and HIV-1 (capsid/p24) protein. SIV and SHIV infected macrophages, potentially dendritic cells and T cells in the testicular interstitial tissue were identified by co-localisation studies using antibodies to CD68, DC-SIGN, αβTCR. Infection of spermatogonia, but not more mature spermatogenic cells, was also observed. Leukocytic infiltrates were observed within the epididymal stroma of the infected animals. CONCLUSION: These data show that the testis and epididymis of juvenile macaques are a target for SIV and SHIV during the post-acute stage of infection and represent a potential model for studying HIV-1 pathogenesis and its effect on spermatogenesis and the MGT in general. BioMed Central 2007-01-31 /pmc/articles/PMC1805449/ /pubmed/17266752 http://dx.doi.org/10.1186/1742-4690-4-7 Text en Copyright © 2007 Shehu-Xhilaga et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Shehu-Xhilaga, Miranda
Kent, Stephen
Batten, Jane
Ellis, Sarah
Van der Meulen, Joel
O'Bryan, Moira
Cameron, Paul U
Lewin, Sharon R
Hedger, Mark P
The testis and epididymis are productively infected by SIV and SHIV in juvenile macaques during the post-acute stage of infection
title The testis and epididymis are productively infected by SIV and SHIV in juvenile macaques during the post-acute stage of infection
title_full The testis and epididymis are productively infected by SIV and SHIV in juvenile macaques during the post-acute stage of infection
title_fullStr The testis and epididymis are productively infected by SIV and SHIV in juvenile macaques during the post-acute stage of infection
title_full_unstemmed The testis and epididymis are productively infected by SIV and SHIV in juvenile macaques during the post-acute stage of infection
title_short The testis and epididymis are productively infected by SIV and SHIV in juvenile macaques during the post-acute stage of infection
title_sort testis and epididymis are productively infected by siv and shiv in juvenile macaques during the post-acute stage of infection
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1805449/
https://www.ncbi.nlm.nih.gov/pubmed/17266752
http://dx.doi.org/10.1186/1742-4690-4-7
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