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Differential response of lymphatic, venous and arterial endothelial cells to angiopoietin-1 and angiopoietin-2

BACKGROUND: The lymphatic system complements the blood circulatory system in absorption and transport of nutrients, and in the maintenance of homeostasis. Angiopoietins 1 and 2 (Ang1 and Ang2) are regulators of both angiogenesis and lymphangiogenesis through the Tek/Tie-2 receptor tyrosine kinase. T...

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Autores principales: Nguyen, Vicky PKH, Chen, Stephen H, Trinh, Jason, Kim, Harold, Coomber, Brenda L, Dumont, Daniel J
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828055/
https://www.ncbi.nlm.nih.gov/pubmed/17341311
http://dx.doi.org/10.1186/1471-2121-8-10
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author Nguyen, Vicky PKH
Chen, Stephen H
Trinh, Jason
Kim, Harold
Coomber, Brenda L
Dumont, Daniel J
author_facet Nguyen, Vicky PKH
Chen, Stephen H
Trinh, Jason
Kim, Harold
Coomber, Brenda L
Dumont, Daniel J
author_sort Nguyen, Vicky PKH
collection PubMed
description BACKGROUND: The lymphatic system complements the blood circulatory system in absorption and transport of nutrients, and in the maintenance of homeostasis. Angiopoietins 1 and 2 (Ang1 and Ang2) are regulators of both angiogenesis and lymphangiogenesis through the Tek/Tie-2 receptor tyrosine kinase. The response of endothelial cells to stimulation with either Ang1 or Ang2 is thought to be dependent upon the origin of the endothelial cells. In this study, we examined the effects of the angiopoietins on lymphatic, venous and arterial primary endothelial cells (bmLEC, bmVEC and bmAEC, respectively), which were isolated and cultured from bovine mesenteric vessels. RESULTS: BmLEC, bmVEC and bmAEC cell populations all express Tie-2 and were shown to express the appropriate cellular markers Prox-1, VEGFR3, and Neuropilin-1 that define the particular origin of each preparation. We showed that while bmLECs responded slightly more readily to angiopoietin-2 (Ang2) stimulation, bmVECs and bmAECs were more sensitive to Ang1 stimulation. Furthermore, exposure of bmLECs to Ang2 induced marginally higher levels of proliferation and survival than did exposure to Ang1. However, exposure to Ang1 resulted in higher levels of migration in bmLECs than did to Ang2. CONCLUSION: Our results suggest that although both Ang1 and Ang2 can activate the Tie-2 receptor in bmLECs, Ang1 and Ang2 may have distinct roles in mesenteric lymphatic endothelial cells.
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spelling pubmed-18280552007-03-16 Differential response of lymphatic, venous and arterial endothelial cells to angiopoietin-1 and angiopoietin-2 Nguyen, Vicky PKH Chen, Stephen H Trinh, Jason Kim, Harold Coomber, Brenda L Dumont, Daniel J BMC Cell Biol Research Article BACKGROUND: The lymphatic system complements the blood circulatory system in absorption and transport of nutrients, and in the maintenance of homeostasis. Angiopoietins 1 and 2 (Ang1 and Ang2) are regulators of both angiogenesis and lymphangiogenesis through the Tek/Tie-2 receptor tyrosine kinase. The response of endothelial cells to stimulation with either Ang1 or Ang2 is thought to be dependent upon the origin of the endothelial cells. In this study, we examined the effects of the angiopoietins on lymphatic, venous and arterial primary endothelial cells (bmLEC, bmVEC and bmAEC, respectively), which were isolated and cultured from bovine mesenteric vessels. RESULTS: BmLEC, bmVEC and bmAEC cell populations all express Tie-2 and were shown to express the appropriate cellular markers Prox-1, VEGFR3, and Neuropilin-1 that define the particular origin of each preparation. We showed that while bmLECs responded slightly more readily to angiopoietin-2 (Ang2) stimulation, bmVECs and bmAECs were more sensitive to Ang1 stimulation. Furthermore, exposure of bmLECs to Ang2 induced marginally higher levels of proliferation and survival than did exposure to Ang1. However, exposure to Ang1 resulted in higher levels of migration in bmLECs than did to Ang2. CONCLUSION: Our results suggest that although both Ang1 and Ang2 can activate the Tie-2 receptor in bmLECs, Ang1 and Ang2 may have distinct roles in mesenteric lymphatic endothelial cells. BioMed Central 2007-03-06 /pmc/articles/PMC1828055/ /pubmed/17341311 http://dx.doi.org/10.1186/1471-2121-8-10 Text en Copyright © 2007 Nguyen et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Nguyen, Vicky PKH
Chen, Stephen H
Trinh, Jason
Kim, Harold
Coomber, Brenda L
Dumont, Daniel J
Differential response of lymphatic, venous and arterial endothelial cells to angiopoietin-1 and angiopoietin-2
title Differential response of lymphatic, venous and arterial endothelial cells to angiopoietin-1 and angiopoietin-2
title_full Differential response of lymphatic, venous and arterial endothelial cells to angiopoietin-1 and angiopoietin-2
title_fullStr Differential response of lymphatic, venous and arterial endothelial cells to angiopoietin-1 and angiopoietin-2
title_full_unstemmed Differential response of lymphatic, venous and arterial endothelial cells to angiopoietin-1 and angiopoietin-2
title_short Differential response of lymphatic, venous and arterial endothelial cells to angiopoietin-1 and angiopoietin-2
title_sort differential response of lymphatic, venous and arterial endothelial cells to angiopoietin-1 and angiopoietin-2
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828055/
https://www.ncbi.nlm.nih.gov/pubmed/17341311
http://dx.doi.org/10.1186/1471-2121-8-10
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