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Apoptosis in the Medaka Embryo in the Early Developmental Stage

Apoptosis is an important event of the development of various organs. In this study, we used in situ terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) to visualize the temporal and spatial distribution of apoptosis in the developing medaka embryo, which is a useful model...

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Detalles Bibliográficos
Autores principales: Iijima, Norio, Yokoyama, Takahiko
Formato: Texto
Lenguaje:English
Publicado: Japan Society of Histochemistry and Cytochemistry 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828077/
https://www.ncbi.nlm.nih.gov/pubmed/17375203
http://dx.doi.org/10.1267/ahc.06013
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author Iijima, Norio
Yokoyama, Takahiko
author_facet Iijima, Norio
Yokoyama, Takahiko
author_sort Iijima, Norio
collection PubMed
description Apoptosis is an important event of the development of various organs. In this study, we used in situ terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) to visualize the temporal and spatial distribution of apoptosis in the developing medaka embryo, which is a useful model for developmental biology and genetics. Most of the apoptotic cells were distributed in the central nervous system and tailbud. In the brain and retina, most of the apoptosis occurred in the restricted period. In situ hybridization against caspase 3A and caspase 3B showed that these were distributed in the tailbud and the head, respectively. These results suggested that two types of caspase 3 were involved in apoptosis in different areas.
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spelling pubmed-18280772007-03-20 Apoptosis in the Medaka Embryo in the Early Developmental Stage Iijima, Norio Yokoyama, Takahiko Acta Histochem Cytochem Regular Article Apoptosis is an important event of the development of various organs. In this study, we used in situ terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) to visualize the temporal and spatial distribution of apoptosis in the developing medaka embryo, which is a useful model for developmental biology and genetics. Most of the apoptotic cells were distributed in the central nervous system and tailbud. In the brain and retina, most of the apoptosis occurred in the restricted period. In situ hybridization against caspase 3A and caspase 3B showed that these were distributed in the tailbud and the head, respectively. These results suggested that two types of caspase 3 were involved in apoptosis in different areas. Japan Society of Histochemistry and Cytochemistry 2007-02-27 2007-01-19 /pmc/articles/PMC1828077/ /pubmed/17375203 http://dx.doi.org/10.1267/ahc.06013 Text en Copyright © 2007 AHC
spellingShingle Regular Article
Iijima, Norio
Yokoyama, Takahiko
Apoptosis in the Medaka Embryo in the Early Developmental Stage
title Apoptosis in the Medaka Embryo in the Early Developmental Stage
title_full Apoptosis in the Medaka Embryo in the Early Developmental Stage
title_fullStr Apoptosis in the Medaka Embryo in the Early Developmental Stage
title_full_unstemmed Apoptosis in the Medaka Embryo in the Early Developmental Stage
title_short Apoptosis in the Medaka Embryo in the Early Developmental Stage
title_sort apoptosis in the medaka embryo in the early developmental stage
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828077/
https://www.ncbi.nlm.nih.gov/pubmed/17375203
http://dx.doi.org/10.1267/ahc.06013
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