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H2A.Z-Mediated Localization of Genes at the Nuclear Periphery Confers Epigenetic Memory of Previous Transcriptional State
Many genes are recruited to the nuclear periphery upon transcriptional activation. The mechanism and functional significance of this recruitment is unclear. We find that recruitment of the yeast INO1 and GAL1 genes to the nuclear periphery is rapid and independent of transcription. Surprisingly, the...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828143/ https://www.ncbi.nlm.nih.gov/pubmed/17373856 http://dx.doi.org/10.1371/journal.pbio.0050081 |
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author | Brickner, Donna Garvey Cajigas, Ivelisse Fondufe-Mittendorf, Yvonne Ahmed, Sara Lee, Pei-Chih Widom, Jonathan Brickner, Jason H |
author_facet | Brickner, Donna Garvey Cajigas, Ivelisse Fondufe-Mittendorf, Yvonne Ahmed, Sara Lee, Pei-Chih Widom, Jonathan Brickner, Jason H |
author_sort | Brickner, Donna Garvey |
collection | PubMed |
description | Many genes are recruited to the nuclear periphery upon transcriptional activation. The mechanism and functional significance of this recruitment is unclear. We find that recruitment of the yeast INO1 and GAL1 genes to the nuclear periphery is rapid and independent of transcription. Surprisingly, these genes remain at the periphery for generations after they are repressed. Localization at the nuclear periphery serves as a form of memory of recent transcriptional activation, promoting reactivation. Previously expressed GAL1 at the nuclear periphery is activated much more rapidly than long-term repressed GAL1 in the nucleoplasm, even after six generations of repression. Localization of INO1 at the nuclear periphery is necessary and sufficient to promote more rapid activation. This form of transcriptional memory is chromatin based; the histone variant H2A.Z is incorporated into nucleosomes within the recently repressed INO1 promoter and is specifically required for rapid reactivation of both INO1 and GAL1. Furthermore, H2A.Z is required to retain INO1 at the nuclear periphery after repression. Therefore, H2A.Z-mediated localization of recently repressed genes at the nuclear periphery represents an epigenetic state that confers memory of transcriptional activation and promotes reactivation. |
format | Text |
id | pubmed-1828143 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-18281432007-05-01 H2A.Z-Mediated Localization of Genes at the Nuclear Periphery Confers Epigenetic Memory of Previous Transcriptional State Brickner, Donna Garvey Cajigas, Ivelisse Fondufe-Mittendorf, Yvonne Ahmed, Sara Lee, Pei-Chih Widom, Jonathan Brickner, Jason H PLoS Biol Research Article Many genes are recruited to the nuclear periphery upon transcriptional activation. The mechanism and functional significance of this recruitment is unclear. We find that recruitment of the yeast INO1 and GAL1 genes to the nuclear periphery is rapid and independent of transcription. Surprisingly, these genes remain at the periphery for generations after they are repressed. Localization at the nuclear periphery serves as a form of memory of recent transcriptional activation, promoting reactivation. Previously expressed GAL1 at the nuclear periphery is activated much more rapidly than long-term repressed GAL1 in the nucleoplasm, even after six generations of repression. Localization of INO1 at the nuclear periphery is necessary and sufficient to promote more rapid activation. This form of transcriptional memory is chromatin based; the histone variant H2A.Z is incorporated into nucleosomes within the recently repressed INO1 promoter and is specifically required for rapid reactivation of both INO1 and GAL1. Furthermore, H2A.Z is required to retain INO1 at the nuclear periphery after repression. Therefore, H2A.Z-mediated localization of recently repressed genes at the nuclear periphery represents an epigenetic state that confers memory of transcriptional activation and promotes reactivation. Public Library of Science 2007-04 2007-03-20 /pmc/articles/PMC1828143/ /pubmed/17373856 http://dx.doi.org/10.1371/journal.pbio.0050081 Text en © 2007 Brickner et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Brickner, Donna Garvey Cajigas, Ivelisse Fondufe-Mittendorf, Yvonne Ahmed, Sara Lee, Pei-Chih Widom, Jonathan Brickner, Jason H H2A.Z-Mediated Localization of Genes at the Nuclear Periphery Confers Epigenetic Memory of Previous Transcriptional State |
title | H2A.Z-Mediated Localization of Genes at the Nuclear Periphery Confers Epigenetic Memory of Previous Transcriptional State |
title_full | H2A.Z-Mediated Localization of Genes at the Nuclear Periphery Confers Epigenetic Memory of Previous Transcriptional State |
title_fullStr | H2A.Z-Mediated Localization of Genes at the Nuclear Periphery Confers Epigenetic Memory of Previous Transcriptional State |
title_full_unstemmed | H2A.Z-Mediated Localization of Genes at the Nuclear Periphery Confers Epigenetic Memory of Previous Transcriptional State |
title_short | H2A.Z-Mediated Localization of Genes at the Nuclear Periphery Confers Epigenetic Memory of Previous Transcriptional State |
title_sort | h2a.z-mediated localization of genes at the nuclear periphery confers epigenetic memory of previous transcriptional state |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828143/ https://www.ncbi.nlm.nih.gov/pubmed/17373856 http://dx.doi.org/10.1371/journal.pbio.0050081 |
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