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H2A.Z-Mediated Localization of Genes at the Nuclear Periphery Confers Epigenetic Memory of Previous Transcriptional State

Many genes are recruited to the nuclear periphery upon transcriptional activation. The mechanism and functional significance of this recruitment is unclear. We find that recruitment of the yeast INO1 and GAL1 genes to the nuclear periphery is rapid and independent of transcription. Surprisingly, the...

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Autores principales: Brickner, Donna Garvey, Cajigas, Ivelisse, Fondufe-Mittendorf, Yvonne, Ahmed, Sara, Lee, Pei-Chih, Widom, Jonathan, Brickner, Jason H
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828143/
https://www.ncbi.nlm.nih.gov/pubmed/17373856
http://dx.doi.org/10.1371/journal.pbio.0050081
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author Brickner, Donna Garvey
Cajigas, Ivelisse
Fondufe-Mittendorf, Yvonne
Ahmed, Sara
Lee, Pei-Chih
Widom, Jonathan
Brickner, Jason H
author_facet Brickner, Donna Garvey
Cajigas, Ivelisse
Fondufe-Mittendorf, Yvonne
Ahmed, Sara
Lee, Pei-Chih
Widom, Jonathan
Brickner, Jason H
author_sort Brickner, Donna Garvey
collection PubMed
description Many genes are recruited to the nuclear periphery upon transcriptional activation. The mechanism and functional significance of this recruitment is unclear. We find that recruitment of the yeast INO1 and GAL1 genes to the nuclear periphery is rapid and independent of transcription. Surprisingly, these genes remain at the periphery for generations after they are repressed. Localization at the nuclear periphery serves as a form of memory of recent transcriptional activation, promoting reactivation. Previously expressed GAL1 at the nuclear periphery is activated much more rapidly than long-term repressed GAL1 in the nucleoplasm, even after six generations of repression. Localization of INO1 at the nuclear periphery is necessary and sufficient to promote more rapid activation. This form of transcriptional memory is chromatin based; the histone variant H2A.Z is incorporated into nucleosomes within the recently repressed INO1 promoter and is specifically required for rapid reactivation of both INO1 and GAL1. Furthermore, H2A.Z is required to retain INO1 at the nuclear periphery after repression. Therefore, H2A.Z-mediated localization of recently repressed genes at the nuclear periphery represents an epigenetic state that confers memory of transcriptional activation and promotes reactivation.
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spelling pubmed-18281432007-05-01 H2A.Z-Mediated Localization of Genes at the Nuclear Periphery Confers Epigenetic Memory of Previous Transcriptional State Brickner, Donna Garvey Cajigas, Ivelisse Fondufe-Mittendorf, Yvonne Ahmed, Sara Lee, Pei-Chih Widom, Jonathan Brickner, Jason H PLoS Biol Research Article Many genes are recruited to the nuclear periphery upon transcriptional activation. The mechanism and functional significance of this recruitment is unclear. We find that recruitment of the yeast INO1 and GAL1 genes to the nuclear periphery is rapid and independent of transcription. Surprisingly, these genes remain at the periphery for generations after they are repressed. Localization at the nuclear periphery serves as a form of memory of recent transcriptional activation, promoting reactivation. Previously expressed GAL1 at the nuclear periphery is activated much more rapidly than long-term repressed GAL1 in the nucleoplasm, even after six generations of repression. Localization of INO1 at the nuclear periphery is necessary and sufficient to promote more rapid activation. This form of transcriptional memory is chromatin based; the histone variant H2A.Z is incorporated into nucleosomes within the recently repressed INO1 promoter and is specifically required for rapid reactivation of both INO1 and GAL1. Furthermore, H2A.Z is required to retain INO1 at the nuclear periphery after repression. Therefore, H2A.Z-mediated localization of recently repressed genes at the nuclear periphery represents an epigenetic state that confers memory of transcriptional activation and promotes reactivation. Public Library of Science 2007-04 2007-03-20 /pmc/articles/PMC1828143/ /pubmed/17373856 http://dx.doi.org/10.1371/journal.pbio.0050081 Text en © 2007 Brickner et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Brickner, Donna Garvey
Cajigas, Ivelisse
Fondufe-Mittendorf, Yvonne
Ahmed, Sara
Lee, Pei-Chih
Widom, Jonathan
Brickner, Jason H
H2A.Z-Mediated Localization of Genes at the Nuclear Periphery Confers Epigenetic Memory of Previous Transcriptional State
title H2A.Z-Mediated Localization of Genes at the Nuclear Periphery Confers Epigenetic Memory of Previous Transcriptional State
title_full H2A.Z-Mediated Localization of Genes at the Nuclear Periphery Confers Epigenetic Memory of Previous Transcriptional State
title_fullStr H2A.Z-Mediated Localization of Genes at the Nuclear Periphery Confers Epigenetic Memory of Previous Transcriptional State
title_full_unstemmed H2A.Z-Mediated Localization of Genes at the Nuclear Periphery Confers Epigenetic Memory of Previous Transcriptional State
title_short H2A.Z-Mediated Localization of Genes at the Nuclear Periphery Confers Epigenetic Memory of Previous Transcriptional State
title_sort h2a.z-mediated localization of genes at the nuclear periphery confers epigenetic memory of previous transcriptional state
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828143/
https://www.ncbi.nlm.nih.gov/pubmed/17373856
http://dx.doi.org/10.1371/journal.pbio.0050081
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