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Activity-dependent Regulation of Dendritic BC1 RNA in Hippocampal Neurons in Culture

Several neuronal RNAs have been identified in dendrites, and it has been suggested that the dendritic location of these RNAs may be relevant to the spatiotemporal regulation of mosaic postsynaptic protein repertoires through transsynaptic activity. Such regulation would require that dendritic RNAs t...

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Detalles Bibliográficos
Autores principales: Muslimov, Ilham A., Banker, Gary, Brosius, Jürgen, Tiedge, Henri
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1998
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828539/
https://www.ncbi.nlm.nih.gov/pubmed/9647652
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author Muslimov, Ilham A.
Banker, Gary
Brosius, Jürgen
Tiedge, Henri
author_facet Muslimov, Ilham A.
Banker, Gary
Brosius, Jürgen
Tiedge, Henri
author_sort Muslimov, Ilham A.
collection PubMed
description Several neuronal RNAs have been identified in dendrites, and it has been suggested that the dendritic location of these RNAs may be relevant to the spatiotemporal regulation of mosaic postsynaptic protein repertoires through transsynaptic activity. Such regulation would require that dendritic RNAs themselves, or at least some of them, be subject to physiological control. We have therefore examined the functional regulation of somatodendritic expression levels of dendritic BC1 RNA in hippocampal neurons in culture. BC1 RNA, an RNA polymerase III transcript that is a component of a ribonucleoprotein particle, became first detectable in somatodendritic domains of developing hippocampal neurons at times of initial synapse formation. BC1 RNA was identified only in such neurons that had established synapses on cell bodies and/or developing dendritic arbors. When synaptic contact formation was initiated later in low-density cultures, BC1 expression was coordinately delayed. Inhibition of neuronal activity in hippocampal neurons resulted in a substantial but reversible reduction of somatodendritic BC1 expression. We conclude that expression of BC1 RNA in somatic and dendritic domains of hippocampal neurons is regulated in development, and is dependent upon neuronal activity. These results establish (for the first time to our knowledge) that an RNA polymerase III transcript can be subject to control through physiological activity in nerve cells.
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spelling pubmed-18285392008-05-01 Activity-dependent Regulation of Dendritic BC1 RNA in Hippocampal Neurons in Culture Muslimov, Ilham A. Banker, Gary Brosius, Jürgen Tiedge, Henri J Cell Biol Articles Several neuronal RNAs have been identified in dendrites, and it has been suggested that the dendritic location of these RNAs may be relevant to the spatiotemporal regulation of mosaic postsynaptic protein repertoires through transsynaptic activity. Such regulation would require that dendritic RNAs themselves, or at least some of them, be subject to physiological control. We have therefore examined the functional regulation of somatodendritic expression levels of dendritic BC1 RNA in hippocampal neurons in culture. BC1 RNA, an RNA polymerase III transcript that is a component of a ribonucleoprotein particle, became first detectable in somatodendritic domains of developing hippocampal neurons at times of initial synapse formation. BC1 RNA was identified only in such neurons that had established synapses on cell bodies and/or developing dendritic arbors. When synaptic contact formation was initiated later in low-density cultures, BC1 expression was coordinately delayed. Inhibition of neuronal activity in hippocampal neurons resulted in a substantial but reversible reduction of somatodendritic BC1 expression. We conclude that expression of BC1 RNA in somatic and dendritic domains of hippocampal neurons is regulated in development, and is dependent upon neuronal activity. These results establish (for the first time to our knowledge) that an RNA polymerase III transcript can be subject to control through physiological activity in nerve cells. The Rockefeller University Press 1998-06-29 /pmc/articles/PMC1828539/ /pubmed/9647652 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Muslimov, Ilham A.
Banker, Gary
Brosius, Jürgen
Tiedge, Henri
Activity-dependent Regulation of Dendritic BC1 RNA in Hippocampal Neurons in Culture
title Activity-dependent Regulation of Dendritic BC1 RNA in Hippocampal Neurons in Culture
title_full Activity-dependent Regulation of Dendritic BC1 RNA in Hippocampal Neurons in Culture
title_fullStr Activity-dependent Regulation of Dendritic BC1 RNA in Hippocampal Neurons in Culture
title_full_unstemmed Activity-dependent Regulation of Dendritic BC1 RNA in Hippocampal Neurons in Culture
title_short Activity-dependent Regulation of Dendritic BC1 RNA in Hippocampal Neurons in Culture
title_sort activity-dependent regulation of dendritic bc1 rna in hippocampal neurons in culture
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828539/
https://www.ncbi.nlm.nih.gov/pubmed/9647652
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