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Gene expression profiling of human mesenchymal stem cells derived from bone marrow during expansion and osteoblast differentiation

BACKGROUND: Human mesenchymal stem cells (MSC) with the capacity to differentiate into osteoblasts provide potential for the development of novel treatment strategies, such as improved healing of large bone defects. However, their low frequency in bone marrow necessitate ex vivo expansion for furthe...

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Autores principales: Kulterer, Birgit, Friedl, Gerald, Jandrositz, Anita, Sanchez-Cabo, Fatima, Prokesch, Andreas, Paar, Christine, Scheideler, Marcel, Windhager, Reinhard, Preisegger, Karl-Heinz, Trajanoski, Zlatko
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1829400/
https://www.ncbi.nlm.nih.gov/pubmed/17352823
http://dx.doi.org/10.1186/1471-2164-8-70
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author Kulterer, Birgit
Friedl, Gerald
Jandrositz, Anita
Sanchez-Cabo, Fatima
Prokesch, Andreas
Paar, Christine
Scheideler, Marcel
Windhager, Reinhard
Preisegger, Karl-Heinz
Trajanoski, Zlatko
author_facet Kulterer, Birgit
Friedl, Gerald
Jandrositz, Anita
Sanchez-Cabo, Fatima
Prokesch, Andreas
Paar, Christine
Scheideler, Marcel
Windhager, Reinhard
Preisegger, Karl-Heinz
Trajanoski, Zlatko
author_sort Kulterer, Birgit
collection PubMed
description BACKGROUND: Human mesenchymal stem cells (MSC) with the capacity to differentiate into osteoblasts provide potential for the development of novel treatment strategies, such as improved healing of large bone defects. However, their low frequency in bone marrow necessitate ex vivo expansion for further clinical application. In this study we asked if MSC are developing in an aberrant or unwanted way during ex vivo long-term cultivation and if artificial cultivation conditions exert any influence on their stem cell maintenance. To address this question we first developed human oligonucleotide microarrays with 30.000 elements and then performed large-scale expression profiling of long-term expanded MSC and MSC during differentiation into osteoblasts. RESULTS: The results showed that MSC did not alter their osteogenic differentiation capacity, surface marker profile, and the expression profiles of MSC during expansion. Microarray analysis of MSC during osteogenic differentiation identified three candidate genes for further examination and functional analysis: ID4, CRYAB, and SORT1. Additionally, we were able to reconstruct the three developmental phases during osteoblast differentiation: proliferation, matrix maturation, and mineralization, and illustrate the activation of the SMAD signaling pathways by TGF-β2 and BMPs. CONCLUSION: With a variety of assays we could show that MSC represent a cell population which can be expanded for therapeutic applications.
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spelling pubmed-18294002007-03-22 Gene expression profiling of human mesenchymal stem cells derived from bone marrow during expansion and osteoblast differentiation Kulterer, Birgit Friedl, Gerald Jandrositz, Anita Sanchez-Cabo, Fatima Prokesch, Andreas Paar, Christine Scheideler, Marcel Windhager, Reinhard Preisegger, Karl-Heinz Trajanoski, Zlatko BMC Genomics Research Article BACKGROUND: Human mesenchymal stem cells (MSC) with the capacity to differentiate into osteoblasts provide potential for the development of novel treatment strategies, such as improved healing of large bone defects. However, their low frequency in bone marrow necessitate ex vivo expansion for further clinical application. In this study we asked if MSC are developing in an aberrant or unwanted way during ex vivo long-term cultivation and if artificial cultivation conditions exert any influence on their stem cell maintenance. To address this question we first developed human oligonucleotide microarrays with 30.000 elements and then performed large-scale expression profiling of long-term expanded MSC and MSC during differentiation into osteoblasts. RESULTS: The results showed that MSC did not alter their osteogenic differentiation capacity, surface marker profile, and the expression profiles of MSC during expansion. Microarray analysis of MSC during osteogenic differentiation identified three candidate genes for further examination and functional analysis: ID4, CRYAB, and SORT1. Additionally, we were able to reconstruct the three developmental phases during osteoblast differentiation: proliferation, matrix maturation, and mineralization, and illustrate the activation of the SMAD signaling pathways by TGF-β2 and BMPs. CONCLUSION: With a variety of assays we could show that MSC represent a cell population which can be expanded for therapeutic applications. BioMed Central 2007-03-12 /pmc/articles/PMC1829400/ /pubmed/17352823 http://dx.doi.org/10.1186/1471-2164-8-70 Text en Copyright © 2007 Kulterer et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kulterer, Birgit
Friedl, Gerald
Jandrositz, Anita
Sanchez-Cabo, Fatima
Prokesch, Andreas
Paar, Christine
Scheideler, Marcel
Windhager, Reinhard
Preisegger, Karl-Heinz
Trajanoski, Zlatko
Gene expression profiling of human mesenchymal stem cells derived from bone marrow during expansion and osteoblast differentiation
title Gene expression profiling of human mesenchymal stem cells derived from bone marrow during expansion and osteoblast differentiation
title_full Gene expression profiling of human mesenchymal stem cells derived from bone marrow during expansion and osteoblast differentiation
title_fullStr Gene expression profiling of human mesenchymal stem cells derived from bone marrow during expansion and osteoblast differentiation
title_full_unstemmed Gene expression profiling of human mesenchymal stem cells derived from bone marrow during expansion and osteoblast differentiation
title_short Gene expression profiling of human mesenchymal stem cells derived from bone marrow during expansion and osteoblast differentiation
title_sort gene expression profiling of human mesenchymal stem cells derived from bone marrow during expansion and osteoblast differentiation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1829400/
https://www.ncbi.nlm.nih.gov/pubmed/17352823
http://dx.doi.org/10.1186/1471-2164-8-70
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