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The Temperature-Sensitive Role of Cryptococcus neoformans ROM2 in Cell Morphogenesis

ROM2 is associated with Cryptococcus neoformans virulence. We examined additional roles of ROM2 in C. neoformans and found that ROM2 plays a role in several cell functions specifically at high temperature conditions. Morphologically rom2 mutant cells demonstrated a “tear”-like shape and clustered to...

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Detalles Bibliográficos
Autores principales: Fuchs, Beth Burgwyn, Tang, Robin J., Mylonakis, Eleftherios
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1838519/
https://www.ncbi.nlm.nih.gov/pubmed/17426816
http://dx.doi.org/10.1371/journal.pone.0000368
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author Fuchs, Beth Burgwyn
Tang, Robin J.
Mylonakis, Eleftherios
author_facet Fuchs, Beth Burgwyn
Tang, Robin J.
Mylonakis, Eleftherios
author_sort Fuchs, Beth Burgwyn
collection PubMed
description ROM2 is associated with Cryptococcus neoformans virulence. We examined additional roles of ROM2 in C. neoformans and found that ROM2 plays a role in several cell functions specifically at high temperature conditions. Morphologically rom2 mutant cells demonstrated a “tear”-like shape and clustered together. A sub-population of cells had a hyperelongated phenotype at restrictive growth conditions. Altered morphology was associated with defects in actin that was concentrated at the cell periphery and with abnormalities in microtubule organization. Interestingly, the ROM2 associated defects in cell morphology, location of nuclei, and actin and microtubule organization were not observed in cells grown at temperatures below 37°C. These results indicate that in C. neoformans, ROM2 is important at restrictive temperature conditions and is involved in several cell maintenance functions.
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spelling pubmed-18385192007-04-11 The Temperature-Sensitive Role of Cryptococcus neoformans ROM2 in Cell Morphogenesis Fuchs, Beth Burgwyn Tang, Robin J. Mylonakis, Eleftherios PLoS One Research Article ROM2 is associated with Cryptococcus neoformans virulence. We examined additional roles of ROM2 in C. neoformans and found that ROM2 plays a role in several cell functions specifically at high temperature conditions. Morphologically rom2 mutant cells demonstrated a “tear”-like shape and clustered together. A sub-population of cells had a hyperelongated phenotype at restrictive growth conditions. Altered morphology was associated with defects in actin that was concentrated at the cell periphery and with abnormalities in microtubule organization. Interestingly, the ROM2 associated defects in cell morphology, location of nuclei, and actin and microtubule organization were not observed in cells grown at temperatures below 37°C. These results indicate that in C. neoformans, ROM2 is important at restrictive temperature conditions and is involved in several cell maintenance functions. Public Library of Science 2007-04-11 /pmc/articles/PMC1838519/ /pubmed/17426816 http://dx.doi.org/10.1371/journal.pone.0000368 Text en Fuchs et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Fuchs, Beth Burgwyn
Tang, Robin J.
Mylonakis, Eleftherios
The Temperature-Sensitive Role of Cryptococcus neoformans ROM2 in Cell Morphogenesis
title The Temperature-Sensitive Role of Cryptococcus neoformans ROM2 in Cell Morphogenesis
title_full The Temperature-Sensitive Role of Cryptococcus neoformans ROM2 in Cell Morphogenesis
title_fullStr The Temperature-Sensitive Role of Cryptococcus neoformans ROM2 in Cell Morphogenesis
title_full_unstemmed The Temperature-Sensitive Role of Cryptococcus neoformans ROM2 in Cell Morphogenesis
title_short The Temperature-Sensitive Role of Cryptococcus neoformans ROM2 in Cell Morphogenesis
title_sort temperature-sensitive role of cryptococcus neoformans rom2 in cell morphogenesis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1838519/
https://www.ncbi.nlm.nih.gov/pubmed/17426816
http://dx.doi.org/10.1371/journal.pone.0000368
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