An annotated cDNA library and microarray for large-scale gene-expression studies in the ant Solenopsis invicta

Ants display a range of fascinating behaviors, a remarkable level of intra-species phenotypic plasticity and many other interesting characteristics. Here we present a new tool to study the molecular mechanisms underlying these traits: a tentatively annotated expressed sequence tag (EST) resource for...

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Autores principales: Wang, John, Jemielity, Stephanie, Uva, Paolo, Wurm, Yannick, Gräff, Johannes, Keller, Laurent
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1839134/
https://www.ncbi.nlm.nih.gov/pubmed/17224046
http://dx.doi.org/10.1186/gb-2007-8-1-r9
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author Wang, John
Jemielity, Stephanie
Uva, Paolo
Wurm, Yannick
Gräff, Johannes
Keller, Laurent
author_facet Wang, John
Jemielity, Stephanie
Uva, Paolo
Wurm, Yannick
Gräff, Johannes
Keller, Laurent
author_sort Wang, John
collection PubMed
description Ants display a range of fascinating behaviors, a remarkable level of intra-species phenotypic plasticity and many other interesting characteristics. Here we present a new tool to study the molecular mechanisms underlying these traits: a tentatively annotated expressed sequence tag (EST) resource for the fire ant Solenopsis invicta. From a normalized cDNA library we obtained 21,715 ESTs, which represent 11,864 putatively different transcripts with very diverse molecular functions. All ESTs were used to construct a cDNA microarray.
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spelling pubmed-18391342007-03-30 An annotated cDNA library and microarray for large-scale gene-expression studies in the ant Solenopsis invicta Wang, John Jemielity, Stephanie Uva, Paolo Wurm, Yannick Gräff, Johannes Keller, Laurent Genome Biol Method Ants display a range of fascinating behaviors, a remarkable level of intra-species phenotypic plasticity and many other interesting characteristics. Here we present a new tool to study the molecular mechanisms underlying these traits: a tentatively annotated expressed sequence tag (EST) resource for the fire ant Solenopsis invicta. From a normalized cDNA library we obtained 21,715 ESTs, which represent 11,864 putatively different transcripts with very diverse molecular functions. All ESTs were used to construct a cDNA microarray. BioMed Central 2007 2007-01-15 /pmc/articles/PMC1839134/ /pubmed/17224046 http://dx.doi.org/10.1186/gb-2007-8-1-r9 Text en Copyright © 2007 Wang et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Method
Wang, John
Jemielity, Stephanie
Uva, Paolo
Wurm, Yannick
Gräff, Johannes
Keller, Laurent
An annotated cDNA library and microarray for large-scale gene-expression studies in the ant Solenopsis invicta
title An annotated cDNA library and microarray for large-scale gene-expression studies in the ant Solenopsis invicta
title_full An annotated cDNA library and microarray for large-scale gene-expression studies in the ant Solenopsis invicta
title_fullStr An annotated cDNA library and microarray for large-scale gene-expression studies in the ant Solenopsis invicta
title_full_unstemmed An annotated cDNA library and microarray for large-scale gene-expression studies in the ant Solenopsis invicta
title_short An annotated cDNA library and microarray for large-scale gene-expression studies in the ant Solenopsis invicta
title_sort annotated cdna library and microarray for large-scale gene-expression studies in the ant solenopsis invicta
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1839134/
https://www.ncbi.nlm.nih.gov/pubmed/17224046
http://dx.doi.org/10.1186/gb-2007-8-1-r9
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