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Distinction between Pore Assembly by Staphylococcal α-Toxin versus Leukotoxins

The staphylococcal bipartite leukotoxins and the homoheptameric α-toxin belong to the same family of β-barrel pore-forming toxins despite slight differences. In the α-toxin pore, the N-terminal extremity of each protomer interacts as a deployed latch with two consecutive protomers in the vicinity of...

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Autores principales: Joubert, Olivier, Voegelin, Joëlle, Guillet, Valérie, Tranier, Samuel, Werner, Sandra, Colin, Didier A., Serra, Mauro Dalla, Keller, Daniel, Monteil, Henri, Mourey, Lionel, Prévost, Gilles
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1847480/
https://www.ncbi.nlm.nih.gov/pubmed/17497023
http://dx.doi.org/10.1155/2007/25935
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author Joubert, Olivier
Voegelin, Joëlle
Guillet, Valérie
Tranier, Samuel
Werner, Sandra
Colin, Didier A.
Serra, Mauro Dalla
Keller, Daniel
Monteil, Henri
Mourey, Lionel
Prévost, Gilles
author_facet Joubert, Olivier
Voegelin, Joëlle
Guillet, Valérie
Tranier, Samuel
Werner, Sandra
Colin, Didier A.
Serra, Mauro Dalla
Keller, Daniel
Monteil, Henri
Mourey, Lionel
Prévost, Gilles
author_sort Joubert, Olivier
collection PubMed
description The staphylococcal bipartite leukotoxins and the homoheptameric α-toxin belong to the same family of β-barrel pore-forming toxins despite slight differences. In the α-toxin pore, the N-terminal extremity of each protomer interacts as a deployed latch with two consecutive protomers in the vicinity of the pore lumen. N-terminal extremities of leukotoxins as seen in their three-dimensional structures are heterogeneous in length and take part in the β-sandwich core of soluble monomers. Hence, the interaction of these N-terminal extremities within structures of adjacent monomers is questionable. We show here that modifications of their N-termini by two different processes, using fusion with glutathione S-transferase (GST) and bridging of the N-terminal extremity to the adjacent β-sheet via disulphide bridges, are not deleterious for biological activity. Therefore, bipartite leukotoxins do not need a large extension of their N-terminal extremities to form functional pores, thus illustrating a microheterogeneity of the structural organizations between bipartite leukotoxins and α-toxin.
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spelling pubmed-18474802007-04-30 Distinction between Pore Assembly by Staphylococcal α-Toxin versus Leukotoxins Joubert, Olivier Voegelin, Joëlle Guillet, Valérie Tranier, Samuel Werner, Sandra Colin, Didier A. Serra, Mauro Dalla Keller, Daniel Monteil, Henri Mourey, Lionel Prévost, Gilles J Biomed Biotechnol Research Article The staphylococcal bipartite leukotoxins and the homoheptameric α-toxin belong to the same family of β-barrel pore-forming toxins despite slight differences. In the α-toxin pore, the N-terminal extremity of each protomer interacts as a deployed latch with two consecutive protomers in the vicinity of the pore lumen. N-terminal extremities of leukotoxins as seen in their three-dimensional structures are heterogeneous in length and take part in the β-sandwich core of soluble monomers. Hence, the interaction of these N-terminal extremities within structures of adjacent monomers is questionable. We show here that modifications of their N-termini by two different processes, using fusion with glutathione S-transferase (GST) and bridging of the N-terminal extremity to the adjacent β-sheet via disulphide bridges, are not deleterious for biological activity. Therefore, bipartite leukotoxins do not need a large extension of their N-terminal extremities to form functional pores, thus illustrating a microheterogeneity of the structural organizations between bipartite leukotoxins and α-toxin. Hindawi Publishing Corporation 2007 2007-02-28 /pmc/articles/PMC1847480/ /pubmed/17497023 http://dx.doi.org/10.1155/2007/25935 Text en Copyright © 2007 Olivier Joubert et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Joubert, Olivier
Voegelin, Joëlle
Guillet, Valérie
Tranier, Samuel
Werner, Sandra
Colin, Didier A.
Serra, Mauro Dalla
Keller, Daniel
Monteil, Henri
Mourey, Lionel
Prévost, Gilles
Distinction between Pore Assembly by Staphylococcal α-Toxin versus Leukotoxins
title Distinction between Pore Assembly by Staphylococcal α-Toxin versus Leukotoxins
title_full Distinction between Pore Assembly by Staphylococcal α-Toxin versus Leukotoxins
title_fullStr Distinction between Pore Assembly by Staphylococcal α-Toxin versus Leukotoxins
title_full_unstemmed Distinction between Pore Assembly by Staphylococcal α-Toxin versus Leukotoxins
title_short Distinction between Pore Assembly by Staphylococcal α-Toxin versus Leukotoxins
title_sort distinction between pore assembly by staphylococcal α-toxin versus leukotoxins
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1847480/
https://www.ncbi.nlm.nih.gov/pubmed/17497023
http://dx.doi.org/10.1155/2007/25935
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