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Use of signal sequences as an in situ removable sequence element to stimulate protein synthesis in cell-free extracts
This study developed a method to boost the expression of recombinant proteins in a cell-free protein synthesis system without leaving additional amino acid residues. It was found that the nucleotide sequences of the signal peptides serve as an efficient downstream box to stimulate protein synthesis...
Autores principales: | Ahn, Jin-Ho, Hwang, Mi-Yeon, Lee, Kyung-Ho, Choi, Cha-Yong, Kim, Dong-Myung |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1849898/ https://www.ncbi.nlm.nih.gov/pubmed/17185295 http://dx.doi.org/10.1093/nar/gkl917 |
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