Cargando…

Cardiovascular Effects of Pulmonary Exposure to Single-Wall Carbon Nanotubes

BACKGROUND: Engineered nanosized materials, such as single-wall carbon nanotubes (SWCNT), are emerging as technologically important in different industries. OBJECTIVE: The unique physical characteristics and the pulmonary toxicity of SWCNTs raised concerns that respiratory exposure to these material...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Zheng, Hulderman, Tracy, Salmen, Rebecca, Chapman, Rebecca, Leonard, Stephen S., Young, Shih-Houng, Shvedova, Anna, Luster, Michael I., Simeonova, Petia P.
Formato: Texto
Lenguaje:English
Publicado: National Institute of Environmental Health Sciences 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1849906/
https://www.ncbi.nlm.nih.gov/pubmed/17431486
http://dx.doi.org/10.1289/ehp.9688
_version_ 1782132939830591488
author Li, Zheng
Hulderman, Tracy
Salmen, Rebecca
Chapman, Rebecca
Leonard, Stephen S.
Young, Shih-Houng
Shvedova, Anna
Luster, Michael I.
Simeonova, Petia P.
author_facet Li, Zheng
Hulderman, Tracy
Salmen, Rebecca
Chapman, Rebecca
Leonard, Stephen S.
Young, Shih-Houng
Shvedova, Anna
Luster, Michael I.
Simeonova, Petia P.
author_sort Li, Zheng
collection PubMed
description BACKGROUND: Engineered nanosized materials, such as single-wall carbon nanotubes (SWCNT), are emerging as technologically important in different industries. OBJECTIVE: The unique physical characteristics and the pulmonary toxicity of SWCNTs raised concerns that respiratory exposure to these materials may be associated with cardiovascular adverse effects. METHODS: In these studies we evaluated aortic mitochondrial alterations by oxidative stress assays, including quantitative polymerase chain reaction of mitochondrial (mt) DNA and plaque formation by morphometric analysis in mice exposed to SWCNTs. RESULTS: A single intrapharyngeal instillation of SWCNTs induced activation of heme oxygenase-1 (HO-1), a marker of oxidative insults, in lung, aorta, and heart tissue in HO-1 reporter transgenic mice. Furthermore, we found that C57BL/6 mice, exposed to SWCNT (10 and 40 μg/mouse), developed aortic mtDNA damage at 7, 28, and 60 days after exposure. mtDNA damage was accompanied by changes in aortic mitochondrial glutathione and protein carbonyl levels. Because these modifications have been related to cardiovascular diseases, we evaluated whether repeated exposure to SWCNTs (20 μg/mouse once every other week for 8 weeks) stimulates the progression of atherosclerosis in ApoE(−/−) transgenic mice. Although SWCNT exposure did not modify the lipid profiles of these mice, it resulted in accelerated plaque formation in ApoE(−/−) mice fed an atherogenic diet. Plaque areas in the aortas, measured by the en face method, and in the brachiocephalic arteries, measured histopathologically, were significantly increased in the SWCNT-treated mice. This response was accompanied by increased mtDNA damage but not inflammation. CONCLUSIONS: Taken together, the findings are of sufficient significance to warrant further studies to evaluate the systemic effects of SWCNT under workplace or environmental exposure paradigms.
format Text
id pubmed-1849906
institution National Center for Biotechnology Information
language English
publishDate 2007
publisher National Institute of Environmental Health Sciences
record_format MEDLINE/PubMed
spelling pubmed-18499062007-04-12 Cardiovascular Effects of Pulmonary Exposure to Single-Wall Carbon Nanotubes Li, Zheng Hulderman, Tracy Salmen, Rebecca Chapman, Rebecca Leonard, Stephen S. Young, Shih-Houng Shvedova, Anna Luster, Michael I. Simeonova, Petia P. Environ Health Perspect Research BACKGROUND: Engineered nanosized materials, such as single-wall carbon nanotubes (SWCNT), are emerging as technologically important in different industries. OBJECTIVE: The unique physical characteristics and the pulmonary toxicity of SWCNTs raised concerns that respiratory exposure to these materials may be associated with cardiovascular adverse effects. METHODS: In these studies we evaluated aortic mitochondrial alterations by oxidative stress assays, including quantitative polymerase chain reaction of mitochondrial (mt) DNA and plaque formation by morphometric analysis in mice exposed to SWCNTs. RESULTS: A single intrapharyngeal instillation of SWCNTs induced activation of heme oxygenase-1 (HO-1), a marker of oxidative insults, in lung, aorta, and heart tissue in HO-1 reporter transgenic mice. Furthermore, we found that C57BL/6 mice, exposed to SWCNT (10 and 40 μg/mouse), developed aortic mtDNA damage at 7, 28, and 60 days after exposure. mtDNA damage was accompanied by changes in aortic mitochondrial glutathione and protein carbonyl levels. Because these modifications have been related to cardiovascular diseases, we evaluated whether repeated exposure to SWCNTs (20 μg/mouse once every other week for 8 weeks) stimulates the progression of atherosclerosis in ApoE(−/−) transgenic mice. Although SWCNT exposure did not modify the lipid profiles of these mice, it resulted in accelerated plaque formation in ApoE(−/−) mice fed an atherogenic diet. Plaque areas in the aortas, measured by the en face method, and in the brachiocephalic arteries, measured histopathologically, were significantly increased in the SWCNT-treated mice. This response was accompanied by increased mtDNA damage but not inflammation. CONCLUSIONS: Taken together, the findings are of sufficient significance to warrant further studies to evaluate the systemic effects of SWCNT under workplace or environmental exposure paradigms. National Institute of Environmental Health Sciences 2007-03 2006-12-04 /pmc/articles/PMC1849906/ /pubmed/17431486 http://dx.doi.org/10.1289/ehp.9688 Text en http://creativecommons.org/publicdomain/mark/1.0/ Publication of EHP lies in the public domain and is therefore without copyright. All text from EHP may be reprinted freely. Use of materials published in EHP should be acknowledged (for example, ?Reproduced with permission from Environmental Health Perspectives?); pertinent reference information should be provided for the article from which the material was reproduced. Articles from EHP, especially the News section, may contain photographs or illustrations copyrighted by other commercial organizations or individuals that may not be used without obtaining prior approval from the holder of the copyright.
spellingShingle Research
Li, Zheng
Hulderman, Tracy
Salmen, Rebecca
Chapman, Rebecca
Leonard, Stephen S.
Young, Shih-Houng
Shvedova, Anna
Luster, Michael I.
Simeonova, Petia P.
Cardiovascular Effects of Pulmonary Exposure to Single-Wall Carbon Nanotubes
title Cardiovascular Effects of Pulmonary Exposure to Single-Wall Carbon Nanotubes
title_full Cardiovascular Effects of Pulmonary Exposure to Single-Wall Carbon Nanotubes
title_fullStr Cardiovascular Effects of Pulmonary Exposure to Single-Wall Carbon Nanotubes
title_full_unstemmed Cardiovascular Effects of Pulmonary Exposure to Single-Wall Carbon Nanotubes
title_short Cardiovascular Effects of Pulmonary Exposure to Single-Wall Carbon Nanotubes
title_sort cardiovascular effects of pulmonary exposure to single-wall carbon nanotubes
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1849906/
https://www.ncbi.nlm.nih.gov/pubmed/17431486
http://dx.doi.org/10.1289/ehp.9688
work_keys_str_mv AT lizheng cardiovasculareffectsofpulmonaryexposuretosinglewallcarbonnanotubes
AT huldermantracy cardiovasculareffectsofpulmonaryexposuretosinglewallcarbonnanotubes
AT salmenrebecca cardiovasculareffectsofpulmonaryexposuretosinglewallcarbonnanotubes
AT chapmanrebecca cardiovasculareffectsofpulmonaryexposuretosinglewallcarbonnanotubes
AT leonardstephens cardiovasculareffectsofpulmonaryexposuretosinglewallcarbonnanotubes
AT youngshihhoung cardiovasculareffectsofpulmonaryexposuretosinglewallcarbonnanotubes
AT shvedovaanna cardiovasculareffectsofpulmonaryexposuretosinglewallcarbonnanotubes
AT lustermichaeli cardiovasculareffectsofpulmonaryexposuretosinglewallcarbonnanotubes
AT simeonovapetiap cardiovasculareffectsofpulmonaryexposuretosinglewallcarbonnanotubes