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Induction of Inflammation in Vascular Endothelial Cells by Metal Oxide Nanoparticles: Effect of Particle Composition

BACKGROUND: The mechanisms governing the correlation between exposure to ultrafine particles and the increased incidence of cardiovascular disease remain unknown. Ultrafine particles appear to cross the pulmonary epithelial barrier into the bloodstream, raising the possibility of direct contact with...

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Autores principales: Gojova, Andrea, Guo, Bing, Kota, Rama S., Rutledge, John C., Kennedy, Ian M., Barakat, Abdul I.
Formato: Texto
Lenguaje:English
Publicado: National Institute of Environmental Health Sciences 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1849911/
https://www.ncbi.nlm.nih.gov/pubmed/17431490
http://dx.doi.org/10.1289/ehp.8497
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author Gojova, Andrea
Guo, Bing
Kota, Rama S.
Rutledge, John C.
Kennedy, Ian M.
Barakat, Abdul I.
author_facet Gojova, Andrea
Guo, Bing
Kota, Rama S.
Rutledge, John C.
Kennedy, Ian M.
Barakat, Abdul I.
author_sort Gojova, Andrea
collection PubMed
description BACKGROUND: The mechanisms governing the correlation between exposure to ultrafine particles and the increased incidence of cardiovascular disease remain unknown. Ultrafine particles appear to cross the pulmonary epithelial barrier into the bloodstream, raising the possibility of direct contact with the vascular endothelium. OBJECTIVES: Because endothelial inflammation is critical for the development of cardiovascular pathology, we hypothesized that direct exposure of human aortic endothelial cells (HAECs) to ultrafine particles induces an inflammatory response and that this response depends on particle composition. METHODS: To test the hypothesis, we incubated HAECs for 1–8 hr with different concentrations (0.001–50 μg/mL) of iron oxide (Fe(2)O(3)), yttrium oxide (Y(2)O(3)), and zinc oxide (ZnO) nanoparticles and subsequently measured mRNA and protein levels of the three inflammatory markers intra-cellular cell adhesion molecule-1, interleukin-8, and monocyte chemotactic protein-1. We also determined nanoparticle interactions with HAECs using inductively coupled plasma mass spectrometry and transmission electron microscopy. RESULTS: Our data indicate that nanoparticle delivery to the HAEC surface and uptake within the cells correlate directly with particle concentration in the cell culture medium. All three types of nanoparticles are internalized into HAECs and are often found within intracellular vesicles. Fe(2)O(3) nanoparticles fail to provoke an inflammatory response in HAECs at any of the concentrations tested; however, Y(2)O(3) and ZnO nanoparticles elicit a pronounced inflammatory response above a threshold concentration of 10 μg/mL. At the highest concentration, ZnO nanoparticles are cytotoxic and lead to considerable cell death. CONCLUSIONS: These results demonstrate that inflammation in HAECs following acute exposure to metal oxide nanoparticles depends on particle composition.
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spelling pubmed-18499112007-04-12 Induction of Inflammation in Vascular Endothelial Cells by Metal Oxide Nanoparticles: Effect of Particle Composition Gojova, Andrea Guo, Bing Kota, Rama S. Rutledge, John C. Kennedy, Ian M. Barakat, Abdul I. Environ Health Perspect Research BACKGROUND: The mechanisms governing the correlation between exposure to ultrafine particles and the increased incidence of cardiovascular disease remain unknown. Ultrafine particles appear to cross the pulmonary epithelial barrier into the bloodstream, raising the possibility of direct contact with the vascular endothelium. OBJECTIVES: Because endothelial inflammation is critical for the development of cardiovascular pathology, we hypothesized that direct exposure of human aortic endothelial cells (HAECs) to ultrafine particles induces an inflammatory response and that this response depends on particle composition. METHODS: To test the hypothesis, we incubated HAECs for 1–8 hr with different concentrations (0.001–50 μg/mL) of iron oxide (Fe(2)O(3)), yttrium oxide (Y(2)O(3)), and zinc oxide (ZnO) nanoparticles and subsequently measured mRNA and protein levels of the three inflammatory markers intra-cellular cell adhesion molecule-1, interleukin-8, and monocyte chemotactic protein-1. We also determined nanoparticle interactions with HAECs using inductively coupled plasma mass spectrometry and transmission electron microscopy. RESULTS: Our data indicate that nanoparticle delivery to the HAEC surface and uptake within the cells correlate directly with particle concentration in the cell culture medium. All three types of nanoparticles are internalized into HAECs and are often found within intracellular vesicles. Fe(2)O(3) nanoparticles fail to provoke an inflammatory response in HAECs at any of the concentrations tested; however, Y(2)O(3) and ZnO nanoparticles elicit a pronounced inflammatory response above a threshold concentration of 10 μg/mL. At the highest concentration, ZnO nanoparticles are cytotoxic and lead to considerable cell death. CONCLUSIONS: These results demonstrate that inflammation in HAECs following acute exposure to metal oxide nanoparticles depends on particle composition. National Institute of Environmental Health Sciences 2007-03 2006-12-11 /pmc/articles/PMC1849911/ /pubmed/17431490 http://dx.doi.org/10.1289/ehp.8497 Text en http://creativecommons.org/publicdomain/mark/1.0/ Publication of EHP lies in the public domain and is therefore without copyright. All text from EHP may be reprinted freely. Use of materials published in EHP should be acknowledged (for example, ?Reproduced with permission from Environmental Health Perspectives?); pertinent reference information should be provided for the article from which the material was reproduced. Articles from EHP, especially the News section, may contain photographs or illustrations copyrighted by other commercial organizations or individuals that may not be used without obtaining prior approval from the holder of the copyright.
spellingShingle Research
Gojova, Andrea
Guo, Bing
Kota, Rama S.
Rutledge, John C.
Kennedy, Ian M.
Barakat, Abdul I.
Induction of Inflammation in Vascular Endothelial Cells by Metal Oxide Nanoparticles: Effect of Particle Composition
title Induction of Inflammation in Vascular Endothelial Cells by Metal Oxide Nanoparticles: Effect of Particle Composition
title_full Induction of Inflammation in Vascular Endothelial Cells by Metal Oxide Nanoparticles: Effect of Particle Composition
title_fullStr Induction of Inflammation in Vascular Endothelial Cells by Metal Oxide Nanoparticles: Effect of Particle Composition
title_full_unstemmed Induction of Inflammation in Vascular Endothelial Cells by Metal Oxide Nanoparticles: Effect of Particle Composition
title_short Induction of Inflammation in Vascular Endothelial Cells by Metal Oxide Nanoparticles: Effect of Particle Composition
title_sort induction of inflammation in vascular endothelial cells by metal oxide nanoparticles: effect of particle composition
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1849911/
https://www.ncbi.nlm.nih.gov/pubmed/17431490
http://dx.doi.org/10.1289/ehp.8497
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